ICESluvan, a 94-Kilobase Mosaic Integrative Conjugative Element Conferring Interspecies Transfer of VanB-Type Glycopeptide Resistance, a Novel Bacitracin Resistance Locus, and a Toxin-Antitoxin Stabilization System
A 94-kb integrative conjugative element (ICESluvan) transferable to Enterococcus faecium and Enterococcus faecalis from an animal isolate of Streptococcus lutetiensis consists of a mosaic of genetic fragments from different Gram-positive bacteria. A variant of ICESluvan was confirmed in S. lutetiens...
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| Veröffentlicht in: | Journal of Bacteriology 2013-12, Vol.195 (23), p.5381-5390 |
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| creator | Bjørkeng, Eva K Hjerde, Erik Pedersen, Torunn Sundsfjord, Arnfinn Hegstad, Kristin |
| description | A 94-kb integrative conjugative element (ICESluvan) transferable to Enterococcus faecium and Enterococcus faecalis from an animal isolate of Streptococcus lutetiensis consists of a mosaic of genetic fragments from different Gram-positive bacteria. A variant of ICESluvan was confirmed in S. lutetiensis from a patient. A complete Tn5382/Tn1549 with a vanB2 operon is integrated into a streptococcal ICESde3396-like region harboring a putative bacteriophage exclusion system, a putative agglutinin receptor precursor, and key components of a type IV secretion system. Moreover, ICESluvan encodes a putative MobC family mobilization protein and a relaxase and, thus, in total has all genetic components essential for conjugative transfer. A 9-kb element within Tn5382/Tn1549 encodes, among others, putative proteins similar to the TnpX site-specific recombinase in Faecalibacterium and VanZ in Paenibacillus, which may contribute to the detected low-level teicoplanin resistance. Furthermore, ICESluvan encodes a novel bacitracin resistance locus that is associated with reduced susceptibility to bacitracin when transferred to E. faecium. The expression of a streptococcal pezAT toxin-antitoxin-encoding operon of ICESluvan in S. lutetiensis, E. faecium, and E. faecalis was confirmed by reverse transcription (RT)-PCR, indicating an active toxin-antitoxin system which may contribute to stabilizing ICESluvan within new hosts. Junction PCR and DNA sequencing confirmed that ICESluvan excised to form a circular intermediate in S. lutetiensis, E. faecalis, and E. faecium. Transfer between E. faecalis cells was observed in the presence of helper plasmid pIP964. Sequence analysis of the original S. lutetiensis donor and enterococcal transconjugants showed that ICESluvan integrates in a site-specific manner into the C-terminal end of the chromosomal tRNA methyltransferase gene rumA. |
| doi_str_mv | 10.1128/JB.02165-12 |
| format | Article |
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A variant of ICESluvan was confirmed in S. lutetiensis from a patient. A complete Tn5382/Tn1549 with a vanB2 operon is integrated into a streptococcal ICESde3396-like region harboring a putative bacteriophage exclusion system, a putative agglutinin receptor precursor, and key components of a type IV secretion system. Moreover, ICESluvan encodes a putative MobC family mobilization protein and a relaxase and, thus, in total has all genetic components essential for conjugative transfer. A 9-kb element within Tn5382/Tn1549 encodes, among others, putative proteins similar to the TnpX site-specific recombinase in Faecalibacterium and VanZ in Paenibacillus, which may contribute to the detected low-level teicoplanin resistance. Furthermore, ICESluvan encodes a novel bacitracin resistance locus that is associated with reduced susceptibility to bacitracin when transferred to E. faecium. The expression of a streptococcal pezAT toxin-antitoxin-encoding operon of ICESluvan in S. lutetiensis, E. faecium, and E. faecalis was confirmed by reverse transcription (RT)-PCR, indicating an active toxin-antitoxin system which may contribute to stabilizing ICESluvan within new hosts. Junction PCR and DNA sequencing confirmed that ICESluvan excised to form a circular intermediate in S. lutetiensis, E. faecalis, and E. faecium. Transfer between E. faecalis cells was observed in the presence of helper plasmid pIP964. Sequence analysis of the original S. lutetiensis donor and enterococcal transconjugants showed that ICESluvan integrates in a site-specific manner into the C-terminal end of the chromosomal tRNA methyltransferase gene rumA.</description><identifier>ISSN: 0021-9193</identifier><identifier>EISSN: 1098-5530</identifier><identifier>EISSN: 1067-8832</identifier><identifier>DOI: 10.1128/JB.02165-12</identifier><identifier>PMID: 24078615</identifier><identifier>CODEN: JOBAAY</identifier><language>eng</language><publisher>United States: American Society for Microbiology</publisher><subject>agglutinins ; animals ; Anti-Bacterial Agents - pharmacology ; Antitoxins - metabolism ; bacitracin ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Bacterial Toxins - metabolism ; Bacteriology ; bacteriophages ; Basale medisinske, odontologiske og veterinærmedisinske fag: 710 ; Base Sequence ; Basic medical, dental and veterinary science disciplines: 710 ; Deoxyribonucleic acid ; DNA ; Enterococcus faecalis ; Enterococcus faecium ; Gene Expression Regulation, Bacterial - physiology ; glycopeptides ; Glycopeptides - pharmacology ; Gram-positive bacteria ; hosts ; loci ; Medical disciplines: 700 ; Medical microbiology: 715 ; Medisinsk mikrobiologi: 715 ; Medisinske Fag: 700 ; Molecular Sequence Data ; Operon ; Paenibacillus ; patients ; Plasmids ; Polymerase chain reaction ; Proteins ; reverse transcriptase polymerase chain reaction ; reverse transcription ; secretion ; sequence analysis ; Streptococcus - genetics ; Streptococcus - metabolism ; Streptococcus lutetiensis ; Toxins ; transcription (genetics) ; Transfer RNA ; Vancomycin - pharmacology ; Vancomycin Resistance - genetics ; VDP</subject><ispartof>Journal of Bacteriology, 2013-12, Vol.195 (23), p.5381-5390</ispartof><rights>Copyright American Society for Microbiology Dec 2013</rights><rights>info:eu-repo/semantics/openAccess</rights><rights>Copyright © 2013, American Society for Microbiology. All Rights Reserved. 2013 American Society for Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3837959/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3837959/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,26567,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/24078615$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bjørkeng, Eva K</creatorcontrib><creatorcontrib>Hjerde, Erik</creatorcontrib><creatorcontrib>Pedersen, Torunn</creatorcontrib><creatorcontrib>Sundsfjord, Arnfinn</creatorcontrib><creatorcontrib>Hegstad, Kristin</creatorcontrib><title>ICESluvan, a 94-Kilobase Mosaic Integrative Conjugative Element Conferring Interspecies Transfer of VanB-Type Glycopeptide Resistance, a Novel Bacitracin Resistance Locus, and a Toxin-Antitoxin Stabilization System</title><title>Journal of Bacteriology</title><addtitle>J Bacteriol</addtitle><description>A 94-kb integrative conjugative element (ICESluvan) transferable to Enterococcus faecium and Enterococcus faecalis from an animal isolate of Streptococcus lutetiensis consists of a mosaic of genetic fragments from different Gram-positive bacteria. A variant of ICESluvan was confirmed in S. lutetiensis from a patient. A complete Tn5382/Tn1549 with a vanB2 operon is integrated into a streptococcal ICESde3396-like region harboring a putative bacteriophage exclusion system, a putative agglutinin receptor precursor, and key components of a type IV secretion system. Moreover, ICESluvan encodes a putative MobC family mobilization protein and a relaxase and, thus, in total has all genetic components essential for conjugative transfer. A 9-kb element within Tn5382/Tn1549 encodes, among others, putative proteins similar to the TnpX site-specific recombinase in Faecalibacterium and VanZ in Paenibacillus, which may contribute to the detected low-level teicoplanin resistance. Furthermore, ICESluvan encodes a novel bacitracin resistance locus that is associated with reduced susceptibility to bacitracin when transferred to E. faecium. The expression of a streptococcal pezAT toxin-antitoxin-encoding operon of ICESluvan in S. lutetiensis, E. faecium, and E. faecalis was confirmed by reverse transcription (RT)-PCR, indicating an active toxin-antitoxin system which may contribute to stabilizing ICESluvan within new hosts. Junction PCR and DNA sequencing confirmed that ICESluvan excised to form a circular intermediate in S. lutetiensis, E. faecalis, and E. faecium. Transfer between E. faecalis cells was observed in the presence of helper plasmid pIP964. Sequence analysis of the original S. lutetiensis donor and enterococcal transconjugants showed that ICESluvan integrates in a site-specific manner into the C-terminal end of the chromosomal tRNA methyltransferase gene rumA.</description><subject>agglutinins</subject><subject>animals</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antitoxins - metabolism</subject><subject>bacitracin</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bacterial Toxins - metabolism</subject><subject>Bacteriology</subject><subject>bacteriophages</subject><subject>Basale medisinske, odontologiske og veterinærmedisinske fag: 710</subject><subject>Base Sequence</subject><subject>Basic medical, dental and veterinary science disciplines: 710</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Enterococcus faecalis</subject><subject>Enterococcus faecium</subject><subject>Gene Expression Regulation, Bacterial - physiology</subject><subject>glycopeptides</subject><subject>Glycopeptides - pharmacology</subject><subject>Gram-positive bacteria</subject><subject>hosts</subject><subject>loci</subject><subject>Medical disciplines: 700</subject><subject>Medical microbiology: 715</subject><subject>Medisinsk mikrobiologi: 715</subject><subject>Medisinske Fag: 700</subject><subject>Molecular Sequence Data</subject><subject>Operon</subject><subject>Paenibacillus</subject><subject>patients</subject><subject>Plasmids</subject><subject>Polymerase chain reaction</subject><subject>Proteins</subject><subject>reverse transcriptase polymerase chain reaction</subject><subject>reverse transcription</subject><subject>secretion</subject><subject>sequence analysis</subject><subject>Streptococcus - genetics</subject><subject>Streptococcus - metabolism</subject><subject>Streptococcus lutetiensis</subject><subject>Toxins</subject><subject>transcription (genetics)</subject><subject>Transfer RNA</subject><subject>Vancomycin - pharmacology</subject><subject>Vancomycin Resistance - genetics</subject><subject>VDP</subject><issn>0021-9193</issn><issn>1098-5530</issn><issn>1067-8832</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>3HK</sourceid><recordid>eNpdkk2P0zAQhiMEYsvCiTtYcOFAFjuuY_uCtK3KUigg0S5Xy3Gc1lViB9splB_K78Ely-fFHs88mnlnPFn2EMELhAr24s3sAhaoJDkqbmUTBDnLCcHwdjaByZ9zxPFZdi-EPYRoOiXF3eysmELKSkQm2fflfLFuh4O0z4EEfJq_Na2rZNDgnQvSKLC0UW-9jOagwdzZ_bAd7UWrO23jyddo743d_kR96LUyOoCNlzakCHAN-CTtLN8cew2u2qNyve6jqTX4qIMJUVqlT7Xfu4NuwUwqE3067F9hsHJqCAmydQI37qux-aWNJp4ssI6yMq35lnS59DqGqLv72Z1GtkE_uLnPs-tXi838db76cLWcX65yVTAcc81KmmZYcFJVjEMkdV3WpWJM0WnR0KqijWwgrxhDUjFcEs1LWmMEkcK1JDU-z16Oefuh6nSt0kS8bEXvTSf9UThpxL8Ra3Zi6w4CM0w54SnBozGB8qlZY4V1XgoEIaaixBgl4NlNBe8-DzpE0ZmgdNtKq90QRPpSTikiDCf06X_o3g3epv4TlQCCKaR_Ko7Cfmv9tRMJeDICO7PdfTFeCxk6sa8E4kQUWBDMTrIej1AjnZDbJF5crwuIyGnJypJg_APi7M76</recordid><startdate>201312</startdate><enddate>201312</enddate><creator>Bjørkeng, Eva K</creator><creator>Hjerde, Erik</creator><creator>Pedersen, Torunn</creator><creator>Sundsfjord, Arnfinn</creator><creator>Hegstad, Kristin</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7QL</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>3HK</scope><scope>5PM</scope></search><sort><creationdate>201312</creationdate><title>ICESluvan, a 94-Kilobase Mosaic Integrative Conjugative Element Conferring Interspecies Transfer of VanB-Type Glycopeptide Resistance, a Novel Bacitracin Resistance Locus, and a Toxin-Antitoxin Stabilization System</title><author>Bjørkeng, Eva K ; Hjerde, Erik ; Pedersen, Torunn ; Sundsfjord, Arnfinn ; Hegstad, Kristin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c283t-e867112295bb8901aed6d6c88c742f7bb7faf09b881ac8365e967d3101c3da5d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>agglutinins</topic><topic>animals</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antitoxins - metabolism</topic><topic>bacitracin</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Bacterial Toxins - metabolism</topic><topic>Bacteriology</topic><topic>bacteriophages</topic><topic>Basale medisinske, odontologiske og veterinærmedisinske fag: 710</topic><topic>Base Sequence</topic><topic>Basic medical, dental and veterinary science disciplines: 710</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Enterococcus faecalis</topic><topic>Enterococcus faecium</topic><topic>Gene Expression Regulation, Bacterial - physiology</topic><topic>glycopeptides</topic><topic>Glycopeptides - pharmacology</topic><topic>Gram-positive bacteria</topic><topic>hosts</topic><topic>loci</topic><topic>Medical disciplines: 700</topic><topic>Medical microbiology: 715</topic><topic>Medisinsk mikrobiologi: 715</topic><topic>Medisinske Fag: 700</topic><topic>Molecular Sequence Data</topic><topic>Operon</topic><topic>Paenibacillus</topic><topic>patients</topic><topic>Plasmids</topic><topic>Polymerase chain reaction</topic><topic>Proteins</topic><topic>reverse transcriptase polymerase chain reaction</topic><topic>reverse transcription</topic><topic>secretion</topic><topic>sequence analysis</topic><topic>Streptococcus - genetics</topic><topic>Streptococcus - metabolism</topic><topic>Streptococcus lutetiensis</topic><topic>Toxins</topic><topic>transcription (genetics)</topic><topic>Transfer RNA</topic><topic>Vancomycin - pharmacology</topic><topic>Vancomycin Resistance - genetics</topic><topic>VDP</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bjørkeng, Eva K</creatorcontrib><creatorcontrib>Hjerde, Erik</creatorcontrib><creatorcontrib>Pedersen, Torunn</creatorcontrib><creatorcontrib>Sundsfjord, Arnfinn</creatorcontrib><creatorcontrib>Hegstad, Kristin</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>NORA - Norwegian Open Research Archives</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Bacteriology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bjørkeng, Eva K</au><au>Hjerde, Erik</au><au>Pedersen, Torunn</au><au>Sundsfjord, Arnfinn</au><au>Hegstad, Kristin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>ICESluvan, a 94-Kilobase Mosaic Integrative Conjugative Element Conferring Interspecies Transfer of VanB-Type Glycopeptide Resistance, a Novel Bacitracin Resistance Locus, and a Toxin-Antitoxin Stabilization System</atitle><jtitle>Journal of Bacteriology</jtitle><addtitle>J Bacteriol</addtitle><date>2013-12</date><risdate>2013</risdate><volume>195</volume><issue>23</issue><spage>5381</spage><epage>5390</epage><pages>5381-5390</pages><issn>0021-9193</issn><eissn>1098-5530</eissn><eissn>1067-8832</eissn><coden>JOBAAY</coden><abstract>A 94-kb integrative conjugative element (ICESluvan) transferable to Enterococcus faecium and Enterococcus faecalis from an animal isolate of Streptococcus lutetiensis consists of a mosaic of genetic fragments from different Gram-positive bacteria. A variant of ICESluvan was confirmed in S. lutetiensis from a patient. A complete Tn5382/Tn1549 with a vanB2 operon is integrated into a streptococcal ICESde3396-like region harboring a putative bacteriophage exclusion system, a putative agglutinin receptor precursor, and key components of a type IV secretion system. Moreover, ICESluvan encodes a putative MobC family mobilization protein and a relaxase and, thus, in total has all genetic components essential for conjugative transfer. A 9-kb element within Tn5382/Tn1549 encodes, among others, putative proteins similar to the TnpX site-specific recombinase in Faecalibacterium and VanZ in Paenibacillus, which may contribute to the detected low-level teicoplanin resistance. Furthermore, ICESluvan encodes a novel bacitracin resistance locus that is associated with reduced susceptibility to bacitracin when transferred to E. faecium. The expression of a streptococcal pezAT toxin-antitoxin-encoding operon of ICESluvan in S. lutetiensis, E. faecium, and E. faecalis was confirmed by reverse transcription (RT)-PCR, indicating an active toxin-antitoxin system which may contribute to stabilizing ICESluvan within new hosts. Junction PCR and DNA sequencing confirmed that ICESluvan excised to form a circular intermediate in S. lutetiensis, E. faecalis, and E. faecium. Transfer between E. faecalis cells was observed in the presence of helper plasmid pIP964. Sequence analysis of the original S. lutetiensis donor and enterococcal transconjugants showed that ICESluvan integrates in a site-specific manner into the C-terminal end of the chromosomal tRNA methyltransferase gene rumA.</abstract><cop>United States</cop><pub>American Society for Microbiology</pub><pmid>24078615</pmid><doi>10.1128/JB.02165-12</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | agglutinins animals Anti-Bacterial Agents - pharmacology Antitoxins - metabolism bacitracin Bacterial Proteins - genetics Bacterial Proteins - metabolism Bacterial Toxins - metabolism Bacteriology bacteriophages Basale medisinske, odontologiske og veterinærmedisinske fag: 710 Base Sequence Basic medical, dental and veterinary science disciplines: 710 Deoxyribonucleic acid DNA Enterococcus faecalis Enterococcus faecium Gene Expression Regulation, Bacterial - physiology glycopeptides Glycopeptides - pharmacology Gram-positive bacteria hosts loci Medical disciplines: 700 Medical microbiology: 715 Medisinsk mikrobiologi: 715 Medisinske Fag: 700 Molecular Sequence Data Operon Paenibacillus patients Plasmids Polymerase chain reaction Proteins reverse transcriptase polymerase chain reaction reverse transcription secretion sequence analysis Streptococcus - genetics Streptococcus - metabolism Streptococcus lutetiensis Toxins transcription (genetics) Transfer RNA Vancomycin - pharmacology Vancomycin Resistance - genetics VDP |
| title | ICESluvan, a 94-Kilobase Mosaic Integrative Conjugative Element Conferring Interspecies Transfer of VanB-Type Glycopeptide Resistance, a Novel Bacitracin Resistance Locus, and a Toxin-Antitoxin Stabilization System |
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