Non‐specific amplification compromises environmental DNA metabarcoding with COI

Metabarcoding extra‐organismal DNA from environmental samples is now a key technique in aquatic biomonitoring and ecosystem health assessment. Of critical consideration when designing experiments, and especially so when developing community standards and legislative frameworks, is the choice of gene...

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Veröffentlicht in:	Methods in ecology and evolution 2019-11, Vol.10 (11), p.1985-2001
Hauptverfasser:	Collins, Rupert A., Bakker, Judith, Wangensteen, Owen S., Soto, Ana Z., Corrigan, Laura, Sims, David W., Genner, Martin J., Mariani, Stefano, Yu, Douglas
Format:	Artikel
Sprache:	eng
Schlagworte:	12S Agriculture and fishery disciplines: 900 Amplification Aquatic ecosystems Biodiversity Biomonitoring COI Cytochrome Cytochrome b Cytochrome-c oxidase Cytochromes Deoxyribonucleic acid DNA DNA barcoding Ecosystem assessment eDNA Environmental DNA Fisheries science: 920 Fiskerifag: 920 Freshwater fish Gene sequencing Genetic markers Genomes In vitro methods and tests Landbruks- og Fiskerifag: 900 Libraries Marine fish Markers metabarcoding Mitochondria Polls & surveys primer design Primers Reproducibility Taxonomy VDP
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