A PRELIMINARY NOTE ON CONSTRUCTING A RICE NUCLEAR GENE LIBRARY

A PRELIMINARY NOTE ON CONSTRUCTING A RICE NUCLEAR GENE LIBRARY

<正> Using the eosmid (pHC 79) as a vector, we have constructed a nuclear gene library of the international rice IR-26 which carries several different disease resistant genes. The rice DNA was extracted and purified from the nuclei, and its molecular weight was determined to be 50-100kb...

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Veröffentlicht in:科学通报：英文版 1982 (3), p.349-350
1. Verfasser: 蔡以欣 谢雍 汪训明 贺沛富 彭泽国 何国顺 张宝珩 阎炳宗 洪仁远 王威
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Sprache:eng
Schlagworte:
library
purified
carries
nuclei
alkali
DNA
totally
ligase
ingle
besides
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Zusammenfassung:<正> Using the eosmid (pHC 79) as a vector, we have constructed a nuclear gene library of the international rice IR-26 which carries several different disease resistant genes. The rice DNA was extracted and purified from the nuclei, and its molecular weight was determined to be 50-100kb. The cosmid DNA was prepared by the method of alkali denaturation. Both rice and
ISSN:2095-9273