Construction and Characterization of Gal-Chitosan Graft Methoxy Poly（Ethylene Glycol）（Gal-CS-mPEG） Nanoparticles as Efficient Gene Carrier
In the present study, galactosylated chitosan（Gal-CS） was conjugated with methoxy poly（ethylene glycol）（m PEG） as a hydrophilic group. The structure of Gal-CS-m PEG polymer was characterized and the nanoparticles（NPs） were prepared using ironic gelation method. The study was designed to investigate...
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| Veröffentlicht in: | 中国海洋大学学报：英文版 2017, Vol.16 (5), p.873-881 |
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| container_title | 中国海洋大学学报：英文版 |
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| creator | JIN Jiting FU Wandong LIAO Miaofei HAN Baoqin CHANG Jing YANG Yan |
| description | In the present study, galactosylated chitosan（Gal-CS） was conjugated with methoxy poly（ethylene glycol）（m PEG） as a hydrophilic group. The structure of Gal-CS-m PEG polymer was characterized and the nanoparticles（NPs） were prepared using ironic gelation method. The study was designed to investigate the characteristics and functions of Gal-CS-m PEG NPs. The morphology of Gal-CS-m PEG NPs was observed by SEM and it was a compact and spherical shape. The size of the NPs was approximately 200 nm in diameter under the ideal process parameters. The interaction between Gal-CS-m PEG NPs and p DNA, and the protection of p DNA against DNase I and serum degradation by Gal-CS-m PEG NPs were evaluated. Agarose gel electrophoresis results showed that Gal-CS-m PEG NPs had strong interaction with p DNA at the weight ratio of 12：1, 4：1 and 2：1 and could protect p DNA from DNase I and serum degradation. Gal-CS-m PEG NPs exhibited high loading efficiency and sustainable in vitro release. The blood compatibility studies demonstrated that Gal-CS-m PEG NPs had superior compatibility with erythrocytes in terms of aggregation degree and hemolysis level. Gal-CS-m PEG NPs showed no cytotoxicity on L929 cells, which is a normal mouse connective tissue fibroblast, but showed inhibitory effects on the proliferation of Bel-7402 cells, which is a liver cancer cell line. In conclusion, Gal-CS-m PEG NP is a bio-safe and efficient gene carrier with potential application in gene delivery. |
| format | Article |
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The structure of Gal-CS-m PEG polymer was characterized and the nanoparticles（NPs） were prepared using ironic gelation method. The study was designed to investigate the characteristics and functions of Gal-CS-m PEG NPs. The morphology of Gal-CS-m PEG NPs was observed by SEM and it was a compact and spherical shape. The size of the NPs was approximately 200 nm in diameter under the ideal process parameters. The interaction between Gal-CS-m PEG NPs and p DNA, and the protection of p DNA against DNase I and serum degradation by Gal-CS-m PEG NPs were evaluated. Agarose gel electrophoresis results showed that Gal-CS-m PEG NPs had strong interaction with p DNA at the weight ratio of 12：1, 4：1 and 2：1 and could protect p DNA from DNase I and serum degradation. Gal-CS-m PEG NPs exhibited high loading efficiency and sustainable in vitro release. The blood compatibility studies demonstrated that Gal-CS-m PEG NPs had superior compatibility with erythrocytes in terms of aggregation degree and hemolysis level. Gal-CS-m PEG NPs showed no cytotoxicity on L929 cells, which is a normal mouse connective tissue fibroblast, but showed inhibitory effects on the proliferation of Bel-7402 cells, which is a liver cancer cell line. In conclusion, Gal-CS-m PEG NP is a bio-safe and efficient gene carrier with potential application in gene delivery.</description><identifier>ISSN: 1672-5182</identifier><identifier>EISSN: 1993-5021</identifier><language>eng</language><subject>DNase ; 半乳糖基 ; 壳聚糖 ; 强相互作用 ; 琼脂糖凝胶电泳 ; 甲氧基聚乙二醇 ; 纳米粒子 ; 表征</subject><ispartof>中国海洋大学学报：英文版, 2017, Vol.16 (5), p.873-881</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/87473A/87473A.jpg</thumbnail><link.rule.ids>314,780,784,4024</link.rule.ids></links><search><creatorcontrib>JIN Jiting FU Wandong LIAO Miaofei HAN Baoqin CHANG Jing YANG Yan</creatorcontrib><title>Construction and Characterization of Gal-Chitosan Graft Methoxy Poly（Ethylene Glycol）（Gal-CS-mPEG） Nanoparticles as Efficient Gene Carrier</title><title>中国海洋大学学报：英文版</title><addtitle>Journal of Ocean University of China</addtitle><description>In the present study, galactosylated chitosan（Gal-CS） was conjugated with methoxy poly（ethylene glycol）（m PEG） as a hydrophilic group. The structure of Gal-CS-m PEG polymer was characterized and the nanoparticles（NPs） were prepared using ironic gelation method. The study was designed to investigate the characteristics and functions of Gal-CS-m PEG NPs. The morphology of Gal-CS-m PEG NPs was observed by SEM and it was a compact and spherical shape. The size of the NPs was approximately 200 nm in diameter under the ideal process parameters. The interaction between Gal-CS-m PEG NPs and p DNA, and the protection of p DNA against DNase I and serum degradation by Gal-CS-m PEG NPs were evaluated. Agarose gel electrophoresis results showed that Gal-CS-m PEG NPs had strong interaction with p DNA at the weight ratio of 12：1, 4：1 and 2：1 and could protect p DNA from DNase I and serum degradation. Gal-CS-m PEG NPs exhibited high loading efficiency and sustainable in vitro release. The blood compatibility studies demonstrated that Gal-CS-m PEG NPs had superior compatibility with erythrocytes in terms of aggregation degree and hemolysis level. Gal-CS-m PEG NPs showed no cytotoxicity on L929 cells, which is a normal mouse connective tissue fibroblast, but showed inhibitory effects on the proliferation of Bel-7402 cells, which is a liver cancer cell line. In conclusion, Gal-CS-m PEG NP is a bio-safe and efficient gene carrier with potential application in gene delivery.</description><subject>DNase</subject><subject>半乳糖基</subject><subject>壳聚糖</subject><subject>强相互作用</subject><subject>琼脂糖凝胶电泳</subject><subject>甲氧基聚乙二醇</subject><subject>纳米粒子</subject><subject>表征</subject><issn>1672-5182</issn><issn>1993-5021</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2017</creationdate><recordtype>article</recordtype><recordid>eNqNi81KAzEURoMoWH_e4eI-kJnpzNh1GONGKdR9ucRME0mTehPB-AS69Xn6Tn0Fx-IDuPo-DuecsFm1WDS8FXV1Ov2ur3lb3dbn7CKlFyHapu36GfuWMaRMbzq7GADDM0iLhDobch94hHEEhZ5L63JMGEARjhkeTLbxvcAy-nLYfw7ZFm-CAeWLjv6w_5rgMVvx7XJQE4BHDHGHlJ32JgEmGMbRaWdCBvWbSiRyhq7Y2Yg-meu_vWQ3d8OTvOfaxrB5dWGz3pHbIpV11zfVfC560fxL-gGpjFvb</recordid><startdate>2017</startdate><enddate>2017</enddate><creator>JIN Jiting FU Wandong LIAO Miaofei HAN Baoqin CHANG Jing YANG Yan</creator><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W94</scope><scope>~WA</scope></search><sort><creationdate>2017</creationdate><title>Construction and Characterization of Gal-Chitosan Graft Methoxy Poly（Ethylene Glycol）（Gal-CS-mPEG） Nanoparticles as Efficient Gene Carrier</title><author>JIN Jiting FU Wandong LIAO Miaofei HAN Baoqin CHANG Jing YANG Yan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-chongqing_primary_6731440703</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2017</creationdate><topic>DNase</topic><topic>半乳糖基</topic><topic>壳聚糖</topic><topic>强相互作用</topic><topic>琼脂糖凝胶电泳</topic><topic>甲氧基聚乙二醇</topic><topic>纳米粒子</topic><topic>表征</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>JIN Jiting FU Wandong LIAO Miaofei HAN Baoqin CHANG Jing YANG Yan</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-自然科学</collection><collection>中文科技期刊数据库- 镜像站点</collection><jtitle>中国海洋大学学报：英文版</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>JIN Jiting FU Wandong LIAO Miaofei HAN Baoqin CHANG Jing YANG Yan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Construction and Characterization of Gal-Chitosan Graft Methoxy Poly（Ethylene Glycol）（Gal-CS-mPEG） Nanoparticles as Efficient Gene Carrier</atitle><jtitle>中国海洋大学学报：英文版</jtitle><addtitle>Journal of Ocean University of China</addtitle><date>2017</date><risdate>2017</risdate><volume>16</volume><issue>5</issue><spage>873</spage><epage>881</epage><pages>873-881</pages><issn>1672-5182</issn><eissn>1993-5021</eissn><abstract>In the present study, galactosylated chitosan（Gal-CS） was conjugated with methoxy poly（ethylene glycol）（m PEG） as a hydrophilic group. The structure of Gal-CS-m PEG polymer was characterized and the nanoparticles（NPs） were prepared using ironic gelation method. The study was designed to investigate the characteristics and functions of Gal-CS-m PEG NPs. The morphology of Gal-CS-m PEG NPs was observed by SEM and it was a compact and spherical shape. The size of the NPs was approximately 200 nm in diameter under the ideal process parameters. The interaction between Gal-CS-m PEG NPs and p DNA, and the protection of p DNA against DNase I and serum degradation by Gal-CS-m PEG NPs were evaluated. Agarose gel electrophoresis results showed that Gal-CS-m PEG NPs had strong interaction with p DNA at the weight ratio of 12：1, 4：1 and 2：1 and could protect p DNA from DNase I and serum degradation. Gal-CS-m PEG NPs exhibited high loading efficiency and sustainable in vitro release. The blood compatibility studies demonstrated that Gal-CS-m PEG NPs had superior compatibility with erythrocytes in terms of aggregation degree and hemolysis level. Gal-CS-m PEG NPs showed no cytotoxicity on L929 cells, which is a normal mouse connective tissue fibroblast, but showed inhibitory effects on the proliferation of Bel-7402 cells, which is a liver cancer cell line. In conclusion, Gal-CS-m PEG NP is a bio-safe and efficient gene carrier with potential application in gene delivery.</abstract></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1672-5182 |
| ispartof | 中国海洋大学学报：英文版, 2017, Vol.16 (5), p.873-881 |
| issn | 1672-5182 1993-5021 |
| language | eng |
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| source | SpringerNature Journals; Alma/SFX Local Collection |
| subjects | DNase 半乳糖基 壳聚糖 强相互作用 琼脂糖凝胶电泳 甲氧基聚乙二醇 纳米粒子 表征 |
| title | Construction and Characterization of Gal-Chitosan Graft Methoxy Poly（Ethylene Glycol）（Gal-CS-mPEG） Nanoparticles as Efficient Gene Carrier |
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