Velvet antler polypeptide is able to induce differentiation of neural stem cells towards neurons in vitro

Velvet antler polypeptide is able to induce differentiation of neural stem cells towards neurons in vitro

OBJECTIVE： To investigate the neural differentiation capacity of water extraction of velvet antler.METHODS： Velvet antler（Cervus Nippon Temminck） polypeptide（VAP） was used to differentiate neural stem cells（NSCs） towards neurons in the study. Firstly, we obtain the polypeptides of VAP by water extra...

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Veröffentlicht in:中医杂志：英文版 2017-06, Vol.37 (3), p.308-313
1. Verfasser: Zhang Lihong Zhuang Zhihong Sun Yanan Ma Shuhua Yang Weifeng Lei Hongtao Zuo Junling Ouyang Jingfeng Wang Yi
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Sprache:eng
Schlagworte:
免疫荧光染色
分化能力
多肽
神经元样细胞
神经干细胞
胶质细胞源性神经营养因子
诱导分化
鹿茸
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container_title 中医杂志：英文版
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creator Zhang Lihong Zhuang Zhihong Sun Yanan Ma Shuhua Yang Weifeng Lei Hongtao Zuo Junling Ouyang Jingfeng Wang Yi
description OBJECTIVE： To investigate the neural differentiation capacity of water extraction of velvet antler.METHODS： Velvet antler（Cervus Nippon Temminck） polypeptide（VAP） was used to differentiate neural stem cells（NSCs） towards neurons in the study. Firstly, we obtain the polypeptides of VAP by water extraction. Secondly, we observed the morphology, assayed the factors in the media by enzyme-linked immunosorbent assay, and detected the special neural molecules by immuno fl uorescence staining. NSCs were cultured on the cell climbing film. After neuronal differentiation, differentiated NSCs were mounted for immunocytochemistry with immunofluorescence technique.RESULTS： The differentiating cells look like neuron,some special factors, such as Glial cell line-derived neurotrophic factor, nerve growth factor, in the media can be detected while differentiated neuron-like cells can express the special neural molecules.CONCLUSION： Differentiation of NSCs towards neurons can be induced by velvet antler polypeptide.
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After neuronal differentiation, differentiated NSCs were mounted for immunocytochemistry with immunofluorescence technique.RESULTS： The differentiating cells look like neuron,some special factors, such as Glial cell line-derived neurotrophic factor, nerve growth factor, in the media can be detected while differentiated neuron-like cells can express the special neural molecules.CONCLUSION： Differentiation of NSCs towards neurons can be induced by velvet antler polypeptide.</description><identifier>ISSN: 0255-2922</identifier><language>eng</language><subject>免疫荧光染色 ; 分化能力 ; 多肽 ; 神经元样细胞 ; 神经干细胞 ; 胶质细胞源性神经营养因子 ; 诱导分化 ; 鹿茸</subject><ispartof>中医杂志：英文版, 2017-06, Vol.37 (3), p.308-313</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/86801X/86801X.jpg</thumbnail><link.rule.ids>314,776,780</link.rule.ids></links><search><creatorcontrib>Zhang Lihong Zhuang Zhihong Sun Yanan Ma Shuhua Yang Weifeng Lei Hongtao Zuo Junling Ouyang Jingfeng Wang Yi</creatorcontrib><title>Velvet antler polypeptide is able to induce differentiation of neural stem cells towards neurons in vitro</title><title>中医杂志：英文版</title><addtitle>Journal of Traditional Chinese Medicine</addtitle><description>OBJECTIVE： To investigate the neural differentiation capacity of water extraction of velvet antler.METHODS： Velvet antler（Cervus Nippon Temminck） polypeptide（VAP） was used to differentiate neural stem cells（NSCs） towards neurons in the study. 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subjects 免疫荧光染色
分化能力
多肽
神经元样细胞
神经干细胞
胶质细胞源性神经营养因子
诱导分化
鹿茸
title Velvet antler polypeptide is able to induce differentiation of neural stem cells towards neurons in vitro
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