Expression of centromere protein-C （CENP-C） in villus tissue of the first-trimester spontaneous abortion and its correlation with chromosome segregation
Objective To investigate the expression of centromere protein-C （CENP-C） in villus tissue of the first-trimester spontaneous abortion （SA） and the correlation study of CENP-C expression with chromosome segregation. Methods Fluorescence in situ hybridization （FISH） and G-banded karyotype analysis wer...
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| creator | Zhi-ping ZHANG Wei-wei XING Bin-bin ZHANG Li-xia LIANG Lei ZHU Xue-qing WU |
| description | Objective To investigate the expression of centromere protein-C （CENP-C） in villus tissue of the first-trimester spontaneous abortion （SA） and the correlation study of CENP-C expression with chromosome segregation. Methods Fluorescence in situ hybridization （FISH） and G-banded karyotype analysis were used to detect the numerical chromosome abnormality in 94 villus tissues of women with SA. The participants were separated into case group （n=30） and control group （n--30） according to the results with FISH. The qRT-PCR and Western blotting analysis were used to assess the expression level of CENP-C. Results Forty-eight （51.06%） cases had observed the numerical chromosome abnormality, including 30positive cases and the positive rate was 31.91%. The main types of variation included trisomy 16, 21, 22, X monosomy and triploid. The expression levels of CENP-C mRNA and protein in case group were statistically higher than that in control group （P〈0.05）. Conclusion Expression of CENP-C in the villus tissues of women might be related to SA induced by chromosomal aneuploid. |
| format | Article |
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Methods Fluorescence in situ hybridization （FISH） and G-banded karyotype analysis were used to detect the numerical chromosome abnormality in 94 villus tissues of women with SA. The participants were separated into case group （n=30） and control group （n--30） according to the results with FISH. The qRT-PCR and Western blotting analysis were used to assess the expression level of CENP-C. Results Forty-eight （51.06%） cases had observed the numerical chromosome abnormality, including 30positive cases and the positive rate was 31.91%. The main types of variation included trisomy 16, 21, 22, X monosomy and triploid. The expression levels of CENP-C mRNA and protein in case group were statistically higher than that in control group （P〈0.05）. Conclusion Expression of CENP-C in the villus tissues of women might be related to SA induced by chromosomal aneuploid.</description><identifier>ISSN: 1001-7844</identifier><language>eng</language><subject>实时荧光定量PCR ; 早期 ; 染色体分离 ; 着丝粒蛋白 ; 组织 ; 绒毛 ; 自然流产 ; 蛋白表达</subject><ispartof>生殖与避孕：英文版, 2016 (1), p.18-24</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/85655X/85655X.jpg</thumbnail><link.rule.ids>314,776,780,4010</link.rule.ids></links><search><creatorcontrib>Zhi-ping ZHANG Wei-wei XING Bin-bin ZHANG Li-xia LIANG Lei ZHU Xue-qing WU</creatorcontrib><title>Expression of centromere protein-C （CENP-C） in villus tissue of the first-trimester spontaneous abortion and its correlation with chromosome segregation</title><title>生殖与避孕：英文版</title><addtitle>Journal of Reproduction and Contraception</addtitle><description>Objective To investigate the expression of centromere protein-C （CENP-C） in villus tissue of the first-trimester spontaneous abortion （SA） and the correlation study of CENP-C expression with chromosome segregation. Methods Fluorescence in situ hybridization （FISH） and G-banded karyotype analysis were used to detect the numerical chromosome abnormality in 94 villus tissues of women with SA. The participants were separated into case group （n=30） and control group （n--30） according to the results with FISH. The qRT-PCR and Western blotting analysis were used to assess the expression level of CENP-C. Results Forty-eight （51.06%） cases had observed the numerical chromosome abnormality, including 30positive cases and the positive rate was 31.91%. The main types of variation included trisomy 16, 21, 22, X monosomy and triploid. The expression levels of CENP-C mRNA and protein in case group were statistically higher than that in control group （P〈0.05）. Conclusion Expression of CENP-C in the villus tissues of women might be related to SA induced by chromosomal aneuploid.</description><subject>实时荧光定量PCR</subject><subject>早期</subject><subject>染色体分离</subject><subject>着丝粒蛋白</subject><subject>组织</subject><subject>绒毛</subject><subject>自然流产</subject><subject>蛋白表达</subject><issn>1001-7844</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNqNjUtOw0AQRGcBEglwhxb7kcZJNAlry4gVYsE-GkzbbuRMm-4OnxvAHXKU3ClXwIk4AKuSql7pnblJEULhl6vF4sJNVV9DiLNlKCZuV30OgqrEGbiBGrMJb1AQBmFDyr6Ew_67rB4efXnY_wBleKe-3yoYqW7x-LIOoSFR8ya0QTUU0IGzpYw8kumZxY6GlF-ATKFmEezTqfsg66DuRivraAbFVrA9bVfuvEm94vVfXrqbu-qpvPd1x7l9o9yuh1GY5Gsd422Yxzhbzf8F_QItfl00</recordid><startdate>2016</startdate><enddate>2016</enddate><creator>Zhi-ping ZHANG Wei-wei XING Bin-bin ZHANG Li-xia LIANG Lei ZHU Xue-qing WU</creator><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope></search><sort><creationdate>2016</creationdate><title>Expression of centromere protein-C （CENP-C） in villus tissue of the first-trimester spontaneous abortion and its correlation with chromosome segregation</title><author>Zhi-ping ZHANG Wei-wei XING Bin-bin ZHANG Li-xia LIANG Lei ZHU Xue-qing WU</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-chongqing_primary_6690366283</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>实时荧光定量PCR</topic><topic>早期</topic><topic>染色体分离</topic><topic>着丝粒蛋白</topic><topic>组织</topic><topic>绒毛</topic><topic>自然流产</topic><topic>蛋白表达</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhi-ping ZHANG Wei-wei XING Bin-bin ZHANG Li-xia LIANG Lei ZHU Xue-qing WU</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><jtitle>生殖与避孕：英文版</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhi-ping ZHANG Wei-wei XING Bin-bin ZHANG Li-xia LIANG Lei ZHU Xue-qing WU</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of centromere protein-C （CENP-C） in villus tissue of the first-trimester spontaneous abortion and its correlation with chromosome segregation</atitle><jtitle>生殖与避孕：英文版</jtitle><addtitle>Journal of Reproduction and Contraception</addtitle><date>2016</date><risdate>2016</risdate><issue>1</issue><spage>18</spage><epage>24</epage><pages>18-24</pages><issn>1001-7844</issn><abstract>Objective To investigate the expression of centromere protein-C （CENP-C） in villus tissue of the first-trimester spontaneous abortion （SA） and the correlation study of CENP-C expression with chromosome segregation. Methods Fluorescence in situ hybridization （FISH） and G-banded karyotype analysis were used to detect the numerical chromosome abnormality in 94 villus tissues of women with SA. The participants were separated into case group （n=30） and control group （n--30） according to the results with FISH. The qRT-PCR and Western blotting analysis were used to assess the expression level of CENP-C. Results Forty-eight （51.06%） cases had observed the numerical chromosome abnormality, including 30positive cases and the positive rate was 31.91%. The main types of variation included trisomy 16, 21, 22, X monosomy and triploid. The expression levels of CENP-C mRNA and protein in case group were statistically higher than that in control group （P〈0.05）. Conclusion Expression of CENP-C in the villus tissues of women might be related to SA induced by chromosomal aneuploid.</abstract></addata></record> |
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| identifier | ISSN: 1001-7844 |
| ispartof | 生殖与避孕：英文版, 2016 (1), p.18-24 |
| issn | 1001-7844 |
| language | eng |
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| source | Elsevier ScienceDirect Journals Complete |
| subjects | 实时荧光定量PCR 早期 染色体分离 着丝粒蛋白 组织 绒毛 自然流产 蛋白表达 |
| title | Expression of centromere protein-C （CENP-C） in villus tissue of the first-trimester spontaneous abortion and its correlation with chromosome segregation |
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