Transcriptional Factor Snail Mediates EpitheliaI-Mesenchymal Transition in Human Bronchial Epithelial Cells Induced by Silica
Epithelial-mesenchymal transition （EMT） plays an important role in fibrotic diseases. We have previously showed that silica induces EMT in human bronchial epithelial cells （BECs）; however, the underlying mechanism of silica-induced EMT is poorly understood. In the present study, we investigated the...
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| Veröffentlicht in: | 生物医学与环境科学：英文版 2015, Vol.28 (7), p.544-548 |
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| container_title | 生物医学与环境科学：英文版 |
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| creator | HU Yong Bin LI Fei Feng DENG Zheng Hao PAN Pin Hua |
| description | Epithelial-mesenchymal transition （EMT） plays an important role in fibrotic diseases. We have previously showed that silica induces EMT in human bronchial epithelial cells （BECs）; however, the underlying mechanism of silica-induced EMT is poorly understood. In the present study, we investigated the role of Snail in silica-induced EMT in human BECs in vitro. Human BECs were treated with silica at various concentrations and incubation times. Then MTr assay, western blot, electrophoretic mobility shift assay （EMSA）, and small interfering RNA （siRNA） transfection were performed. We found that silica increased the expression and DNA binding activity of Snail in human BECs. SNAI silica-induced expression siRNA upregulated the siRNA inhibited the of Snail. Moreover, SNAI expression of epithelial marker E-cadherin, but attenuated the expression of mesenchymal marker a-smooth muscle actin and vimentin in silica-stimulated cells. These results suggest that Snail mediates the silica-induced EMT in human BECs. |
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We have previously showed that silica induces EMT in human bronchial epithelial cells （BECs）; however, the underlying mechanism of silica-induced EMT is poorly understood. In the present study, we investigated the role of Snail in silica-induced EMT in human BECs in vitro. Human BECs were treated with silica at various concentrations and incubation times. Then MTr assay, western blot, electrophoretic mobility shift assay （EMSA）, and small interfering RNA （siRNA） transfection were performed. We found that silica increased the expression and DNA binding activity of Snail in human BECs. SNAI silica-induced expression siRNA upregulated the siRNA inhibited the of Snail. Moreover, SNAI expression of epithelial marker E-cadherin, but attenuated the expression of mesenchymal marker a-smooth muscle actin and vimentin in silica-stimulated cells. 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We have previously showed that silica induces EMT in human bronchial epithelial cells （BECs）; however, the underlying mechanism of silica-induced EMT is poorly understood. In the present study, we investigated the role of Snail in silica-induced EMT in human BECs in vitro. Human BECs were treated with silica at various concentrations and incubation times. Then MTr assay, western blot, electrophoretic mobility shift assay （EMSA）, and small interfering RNA （siRNA） transfection were performed. We found that silica increased the expression and DNA binding activity of Snail in human BECs. SNAI silica-induced expression siRNA upregulated the siRNA inhibited the of Snail. Moreover, SNAI expression of epithelial marker E-cadherin, but attenuated the expression of mesenchymal marker a-smooth muscle actin and vimentin in silica-stimulated cells. These results suggest that Snail mediates the silica-induced EMT in human BECs.</description><subject>siRNA</subject><subject>α-平滑肌肌动蛋白</subject><subject>介导</subject><subject>支气管上皮细胞</subject><subject>诱导表达</subject><subject>转化</subject><subject>转录因子</subject><subject>间质</subject><issn>0895-3988</issn><issn>2214-0190</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNqNi7FugzAUAK2olULT_sNTdyQ7EAprEAiGTMkevRg3vMo8iA0DQ_-9NIo6d7rh7lYi2G5VHEqVyScRyDTbhVGWpmvx4v2XlLHK4jQQ3yeH7LWjYaSe0UKJeuwdHBnJwsE0hKPxUAw0tsYS1uHBeMO6nbslvs_0ewIxVFOHDHvXL5oW-zdZyI21HmpuJm0auMxwJEsaX8XzJ1pv3h7ciPeyOOVVqNuerzfi63lw1KGbz0my-1BRouLoX9EP4HtQLA</recordid><startdate>2015</startdate><enddate>2015</enddate><creator>HU Yong Bin LI Fei Feng DENG Zheng Hao PAN Pin Hua</creator><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope></search><sort><creationdate>2015</creationdate><title>Transcriptional Factor Snail Mediates EpitheliaI-Mesenchymal Transition in Human Bronchial Epithelial Cells Induced by Silica</title><author>HU Yong Bin LI Fei Feng DENG Zheng Hao PAN Pin Hua</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-chongqing_primary_6657136143</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>siRNA</topic><topic>α-平滑肌肌动蛋白</topic><topic>介导</topic><topic>支气管上皮细胞</topic><topic>诱导表达</topic><topic>转化</topic><topic>转录因子</topic><topic>间质</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>HU Yong Bin LI Fei Feng DENG Zheng Hao PAN Pin Hua</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><jtitle>生物医学与环境科学：英文版</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>HU Yong Bin LI Fei Feng DENG Zheng Hao PAN Pin Hua</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transcriptional Factor Snail Mediates EpitheliaI-Mesenchymal Transition in Human Bronchial Epithelial Cells Induced by Silica</atitle><jtitle>生物医学与环境科学：英文版</jtitle><addtitle>Biomedical and Environmental Sciences</addtitle><date>2015</date><risdate>2015</risdate><volume>28</volume><issue>7</issue><spage>544</spage><epage>548</epage><pages>544-548</pages><issn>0895-3988</issn><eissn>2214-0190</eissn><abstract>Epithelial-mesenchymal transition （EMT） plays an important role in fibrotic diseases. We have previously showed that silica induces EMT in human bronchial epithelial cells （BECs）; however, the underlying mechanism of silica-induced EMT is poorly understood. In the present study, we investigated the role of Snail in silica-induced EMT in human BECs in vitro. Human BECs were treated with silica at various concentrations and incubation times. Then MTr assay, western blot, electrophoretic mobility shift assay （EMSA）, and small interfering RNA （siRNA） transfection were performed. We found that silica increased the expression and DNA binding activity of Snail in human BECs. SNAI silica-induced expression siRNA upregulated the siRNA inhibited the of Snail. Moreover, SNAI expression of epithelial marker E-cadherin, but attenuated the expression of mesenchymal marker a-smooth muscle actin and vimentin in silica-stimulated cells. These results suggest that Snail mediates the silica-induced EMT in human BECs.</abstract></addata></record> |
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| ispartof | 生物医学与环境科学：英文版, 2015, Vol.28 (7), p.544-548 |
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| source | Elsevier ScienceDirect Journals; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | siRNA α-平滑肌肌动蛋白 介导 支气管上皮细胞 诱导表达 转化 转录因子 间质 |
| title | Transcriptional Factor Snail Mediates EpitheliaI-Mesenchymal Transition in Human Bronchial Epithelial Cells Induced by Silica |
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