Expression Analysis of the Insulin-Like Growth Factors Ⅰ and Ⅱ During Embryonic and Early Larval Development of Turbot （Scophthalmus maximus）
The insulin-like growth factors Ⅰ and Ⅱ （IGF-Ⅰ and IGF-Ⅱ） are important proteins involved in fish growth and develop- ment. Here, we report the isolation of IGF-Ⅱ and expression analysis of IGFs in turbot Scophthalmus maximus, aiming to clarify their function in embryonic and larval development of f...
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| creator | WEN Haishen QI Qian HU Jian SI Yufeng HE Feng LI Jifang |
| description | The insulin-like growth factors Ⅰ and Ⅱ （IGF-Ⅰ and IGF-Ⅱ） are important proteins involved in fish growth and develop- ment. Here, we report the isolation of IGF-Ⅱ and expression analysis of IGFs in turbot Scophthalmus maximus, aiming to clarify their function in embryonic and larval development of fish. The deduced IGF-Ⅱ gene is 808 bp in full length, which encodes a protein of 219 amino acids and is 93% similar with that ofParalichthys olicaceus in amino acid sequence. The tissue abundance and the ex- pression pattern of IGFs in a turbot at early development stages were investigated via reverse transcription-polymer chain reaction. Result showed that the IGF-Ⅰ and IGF-Ⅱ genes were widely expressed in tissues of S. maximus. IGF-Ⅰ was detected in all tissues ex- cept intestines with the highest level in liver, while IGF-Ⅱ transcript presented in all tissues except muscle. At the stages of embry- onic and larval development, the mRNA levels of IGFs sharply increased from the stage of unfertilized egg to post larva, followed by a decrease with larval development. However, there was an increase in IGF-Ⅰ at the embryonic stage and IGF-Ⅱ at the gastrula stage, respectively. These results suggested that IGFs play important roles in cell growth and division of the turbot. Our study provides reference data for further investigation of growth regulation in turbot, which can guarantee better understanding of the physiological role that IGFs play in fish. |
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Here, we report the isolation of IGF-Ⅱ and expression analysis of IGFs in turbot Scophthalmus maximus, aiming to clarify their function in embryonic and larval development of fish. The deduced IGF-Ⅱ gene is 808 bp in full length, which encodes a protein of 219 amino acids and is 93% similar with that ofParalichthys olicaceus in amino acid sequence. The tissue abundance and the ex- pression pattern of IGFs in a turbot at early development stages were investigated via reverse transcription-polymer chain reaction. Result showed that the IGF-Ⅰ and IGF-Ⅱ genes were widely expressed in tissues of S. maximus. IGF-Ⅰ was detected in all tissues ex- cept intestines with the highest level in liver, while IGF-Ⅱ transcript presented in all tissues except muscle. At the stages of embry- onic and larval development, the mRNA levels of IGFs sharply increased from the stage of unfertilized egg to post larva, followed by a decrease with larval development. However, there was an increase in IGF-Ⅰ at the embryonic stage and IGF-Ⅱ at the gastrula stage, respectively. These results suggested that IGFs play important roles in cell growth and division of the turbot. 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However, there was an increase in IGF-Ⅰ at the embryonic stage and IGF-Ⅱ at the gastrula stage, respectively. These results suggested that IGFs play important roles in cell growth and division of the turbot. Our study provides reference data for further investigation of growth regulation in turbot, which can guarantee better understanding of the physiological role that IGFs play in fish.</description><subject>大菱鲆</subject><subject>幼体发育</subject><subject>早期发展</subject><subject>氨基酸序列</subject><subject>肌肉组织</subject><subject>胚胎期</subject><subject>胰岛素样生长因子</subject><subject>表达分析</subject><issn>1672-5182</issn><issn>1993-5021</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNqNjUtOw0AQRC0EEuFzhxZ7S_5kHLxExAGk7Mg-6phJZmA-pnsc4gMgwQHYh3vkNFwgV8AgDsDqlVRPVQfRIC3LPBZJlh72uRhlsUgvs-PohPkxSUQuitEg-qg2DUlm7R1cOTQdawa_hKAk3DlujXbxVD9JuCH_EhRMsA6eGL5et4DuoecnjFvSbgWVXVDnna5_iwrJdDBFWqOBsVxL4xsrXfgZn7W08AH2u7f72jcqKDS2ZbC40T33u_ez6GiJhuX5H0-ji0k1u76Na-Xd6rl_mzekLVI3L4phLkRaDvN_Sd8zwFzm</recordid><startdate>2015</startdate><enddate>2015</enddate><creator>WEN Haishen QI Qian HU Jian SI Yufeng HE Feng LI Jifang</creator><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W94</scope><scope>~WA</scope></search><sort><creationdate>2015</creationdate><title>Expression Analysis of the Insulin-Like Growth Factors Ⅰ and Ⅱ During Embryonic and Early Larval Development of Turbot （Scophthalmus maximus）</title><author>WEN Haishen QI Qian HU Jian SI Yufeng HE Feng LI Jifang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-chongqing_primary_6643551943</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>大菱鲆</topic><topic>幼体发育</topic><topic>早期发展</topic><topic>氨基酸序列</topic><topic>肌肉组织</topic><topic>胚胎期</topic><topic>胰岛素样生长因子</topic><topic>表达分析</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>WEN Haishen QI Qian HU Jian SI Yufeng HE Feng LI Jifang</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-自然科学</collection><collection>中文科技期刊数据库- 镜像站点</collection><jtitle>中国海洋大学学报：英文版</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>WEN Haishen QI Qian HU Jian SI Yufeng HE Feng LI Jifang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression Analysis of the Insulin-Like Growth Factors Ⅰ and Ⅱ During Embryonic and Early Larval Development of Turbot （Scophthalmus maximus）</atitle><jtitle>中国海洋大学学报：英文版</jtitle><addtitle>Journal of Ocean University of China</addtitle><date>2015</date><risdate>2015</risdate><issue>2</issue><spage>309</spage><epage>316</epage><pages>309-316</pages><issn>1672-5182</issn><eissn>1993-5021</eissn><abstract>The insulin-like growth factors Ⅰ and Ⅱ （IGF-Ⅰ and IGF-Ⅱ） are important proteins involved in fish growth and develop- ment. 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However, there was an increase in IGF-Ⅰ at the embryonic stage and IGF-Ⅱ at the gastrula stage, respectively. These results suggested that IGFs play important roles in cell growth and division of the turbot. Our study provides reference data for further investigation of growth regulation in turbot, which can guarantee better understanding of the physiological role that IGFs play in fish.</abstract></addata></record> |
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| identifier | ISSN: 1672-5182 |
| ispartof | 中国海洋大学学报：英文版, 2015 (2), p.309-316 |
| issn | 1672-5182 1993-5021 |
| language | eng |
| recordid | cdi_chongqing_primary_664355194 |
| source | SpringerNature Journals; Alma/SFX Local Collection |
| subjects | 大菱鲆 幼体发育 早期发展 氨基酸序列 肌肉组织 胚胎期 胰岛素样生长因子 表达分析 |
| title | Expression Analysis of the Insulin-Like Growth Factors Ⅰ and Ⅱ During Embryonic and Early Larval Development of Turbot （Scophthalmus maximus） |
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