Aqueous extract of Taxus Chinensis (Pilger) Rehd inhibits lung carci- noma A549 cells through the epidermal growth factor receptor/mi- togen-activated protein kinase pathway in vitro and in vivo
OBJECTIVE: To explore the anticancer mechanism of aqueous extract of Taxus Chinensis (Pilger) Rehd (AETC). METHODS: The serum pharmacological method was used to avoid interference from administration of the crude medicinal herbs. Eight purebred NewZealand rabbits were used for preparation of serum c...
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description | OBJECTIVE: To explore the anticancer mechanism of aqueous extract of Taxus Chinensis (Pilger) Rehd (AETC). METHODS: The serum pharmacological method was used to avoid interference from administration of the crude medicinal herbs. Eight purebred NewZealand rabbits were used for preparation of serum containing various concentrations of AETC. For- ty-eight Balb/c-nu mice were used for in vivo experi- ments. The effects of serum containing AETC on the proliferation of A549 cells and expression levels of the epidermal growth factor receptor/mito- gen-activated protein kinase (EGFR/MAPK) path- way-related proteins in vitro were investigated. Ad- ditionally, the effects on the growth of A549 xeno- grafts in nude mice, and expression levels of the EG- FR/MAPK pathway-related proteins in the xeno- grafts, were investigated. RESULTS: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl- tetrazolium bromide (MTT) assay revealed that the serum containing AETC significantly decreased the viability of A549 cells in a dose-dependent manner. Western blot showed that the serum containing various concentrations of AETC strongly reduced the levels of phospho-Jun N-terminal kinase (p-JNK) and phospho-extracellular signal-regulated kinasel/2 (ERK1/2) while it increased the level of p-p38. However, no significant effects on the ex- pression levels of JNK, ERK1/2, and p38 MAPK were found. In addition, an anticancer effect from AETC was observed in vivo in the Balb/c-nu mice bearing A549 xenografts.CONCLUSION: AETC has significant effects on the growth of A549 xenografts and on the activity of the EGFR/MAPK pathway. Therefore, AETC may be beneficial in lung carcinoma treatment. |
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METHODS: The serum pharmacological method was used to avoid interference from administration of the crude medicinal herbs. Eight purebred NewZealand rabbits were used for preparation of serum containing various concentrations of AETC. For- ty-eight Balb/c-nu mice were used for in vivo experi- ments. The effects of serum containing AETC on the proliferation of A549 cells and expression levels of the epidermal growth factor receptor/mito- gen-activated protein kinase (EGFR/MAPK) path- way-related proteins in vitro were investigated. Ad- ditionally, the effects on the growth of A549 xeno- grafts in nude mice, and expression levels of the EG- FR/MAPK pathway-related proteins in the xeno- grafts, were investigated. RESULTS: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl- tetrazolium bromide (MTT) assay revealed that the serum containing AETC significantly decreased the viability of A549 cells in a dose-dependent manner. Western blot showed that the serum containing various concentrations of AETC strongly reduced the levels of phospho-Jun N-terminal kinase (p-JNK) and phospho-extracellular signal-regulated kinasel/2 (ERK1/2) while it increased the level of p-p38. However, no significant effects on the ex- pression levels of JNK, ERK1/2, and p38 MAPK were found. In addition, an anticancer effect from AETC was observed in vivo in the Balb/c-nu mice bearing A549 xenografts.CONCLUSION: AETC has significant effects on the growth of A549 xenografts and on the activity of the EGFR/MAPK pathway. Therefore, AETC may be beneficial in lung carcinoma treatment.</description><identifier>ISSN: 0255-2922</identifier><language>eng</language><subject>A549细胞 ; 体内实验 ; 体外 ; 水提取物 ; 皮下埋植 ; 红豆杉 ; 蛋白激酶途径 ; 表皮生长因子受体</subject><ispartof>中医杂志:英文版, 2014 (3), p.293-301</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/86801X/86801X.jpg</thumbnail><link.rule.ids>314,778,782,4012</link.rule.ids></links><search><creatorcontrib>Qijin Shu Minhe Shen Binbin Wang Qingli Cui Xiaoying Zhou Luming Zhu</creatorcontrib><title>Aqueous extract of Taxus Chinensis (Pilger) Rehd inhibits lung carci- noma A549 cells through the epidermal growth factor receptor/mi- togen-activated protein kinase pathway in vitro and in vivo</title><title>中医杂志:英文版</title><addtitle>Journal of Traditional Chinese Medicine</addtitle><description>OBJECTIVE: To explore the anticancer mechanism of aqueous extract of Taxus Chinensis (Pilger) Rehd (AETC). METHODS: The serum pharmacological method was used to avoid interference from administration of the crude medicinal herbs. Eight purebred NewZealand rabbits were used for preparation of serum containing various concentrations of AETC. For- ty-eight Balb/c-nu mice were used for in vivo experi- ments. The effects of serum containing AETC on the proliferation of A549 cells and expression levels of the epidermal growth factor receptor/mito- gen-activated protein kinase (EGFR/MAPK) path- way-related proteins in vitro were investigated. Ad- ditionally, the effects on the growth of A549 xeno- grafts in nude mice, and expression levels of the EG- FR/MAPK pathway-related proteins in the xeno- grafts, were investigated. RESULTS: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl- tetrazolium bromide (MTT) assay revealed that the serum containing AETC significantly decreased the viability of A549 cells in a dose-dependent manner. Western blot showed that the serum containing various concentrations of AETC strongly reduced the levels of phospho-Jun N-terminal kinase (p-JNK) and phospho-extracellular signal-regulated kinasel/2 (ERK1/2) while it increased the level of p-p38. However, no significant effects on the ex- pression levels of JNK, ERK1/2, and p38 MAPK were found. In addition, an anticancer effect from AETC was observed in vivo in the Balb/c-nu mice bearing A549 xenografts.CONCLUSION: AETC has significant effects on the growth of A549 xenografts and on the activity of the EGFR/MAPK pathway. Therefore, AETC may be beneficial in lung carcinoma treatment.</description><subject>A549细胞</subject><subject>体内实验</subject><subject>体外</subject><subject>水提取物</subject><subject>皮下埋植</subject><subject>红豆杉</subject><subject>蛋白激酶途径</subject><subject>表皮生长因子受体</subject><issn>0255-2922</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNqNTUtOwzAQzQIkyucOwwEi0oBFs6wqEEuEuq8GZ2IPOHY6dvq5Adypa67TK2CJHoDV--q9s2JS1UqVdVPXF8VljB9VpWZKzSbFz3w9Uhgj0C4J6gShgyXusrGw7MlHjnA8fL2yMyTHwze8kW2BveV3ThHc6A1oFM0l-NAjzNVDA5qci5CshNHYjAQ0cEvSowMjYZssdPkrCAhpGjK56_NACoZ8mQPeYKIWBgmJ2MMne4wEAya7xX0-hw0nCYC-_RObcF2cd-gi3Zzwqrh9flouXkptgzdr9mY1CPco-5Wqpo9N3Uzv_9P5BRS2as8</recordid><startdate>2014</startdate><enddate>2014</enddate><creator>Qijin Shu Minhe Shen Binbin Wang Qingli Cui Xiaoying Zhou Luming Zhu</creator><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope></search><sort><creationdate>2014</creationdate><title>Aqueous extract of Taxus Chinensis (Pilger) Rehd inhibits lung carci- noma A549 cells through the epidermal growth factor receptor/mi- togen-activated protein kinase pathway in vitro and in vivo</title><author>Qijin Shu Minhe Shen Binbin Wang Qingli Cui Xiaoying Zhou Luming Zhu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-chongqing_primary_501792913</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>A549细胞</topic><topic>体内实验</topic><topic>体外</topic><topic>水提取物</topic><topic>皮下埋植</topic><topic>红豆杉</topic><topic>蛋白激酶途径</topic><topic>表皮生长因子受体</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Qijin Shu Minhe Shen Binbin Wang Qingli Cui Xiaoying Zhou Luming Zhu</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><jtitle>中医杂志:英文版</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Qijin Shu Minhe Shen Binbin Wang Qingli Cui Xiaoying Zhou Luming Zhu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Aqueous extract of Taxus Chinensis (Pilger) Rehd inhibits lung carci- noma A549 cells through the epidermal growth factor receptor/mi- togen-activated protein kinase pathway in vitro and in vivo</atitle><jtitle>中医杂志:英文版</jtitle><addtitle>Journal of Traditional Chinese Medicine</addtitle><date>2014</date><risdate>2014</risdate><issue>3</issue><spage>293</spage><epage>301</epage><pages>293-301</pages><issn>0255-2922</issn><abstract>OBJECTIVE: To explore the anticancer mechanism of aqueous extract of Taxus Chinensis (Pilger) Rehd (AETC). METHODS: The serum pharmacological method was used to avoid interference from administration of the crude medicinal herbs. Eight purebred NewZealand rabbits were used for preparation of serum containing various concentrations of AETC. For- ty-eight Balb/c-nu mice were used for in vivo experi- ments. The effects of serum containing AETC on the proliferation of A549 cells and expression levels of the epidermal growth factor receptor/mito- gen-activated protein kinase (EGFR/MAPK) path- way-related proteins in vitro were investigated. Ad- ditionally, the effects on the growth of A549 xeno- grafts in nude mice, and expression levels of the EG- FR/MAPK pathway-related proteins in the xeno- grafts, were investigated. RESULTS: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl- tetrazolium bromide (MTT) assay revealed that the serum containing AETC significantly decreased the viability of A549 cells in a dose-dependent manner. Western blot showed that the serum containing various concentrations of AETC strongly reduced the levels of phospho-Jun N-terminal kinase (p-JNK) and phospho-extracellular signal-regulated kinasel/2 (ERK1/2) while it increased the level of p-p38. However, no significant effects on the ex- pression levels of JNK, ERK1/2, and p38 MAPK were found. In addition, an anticancer effect from AETC was observed in vivo in the Balb/c-nu mice bearing A549 xenografts.CONCLUSION: AETC has significant effects on the growth of A549 xenografts and on the activity of the EGFR/MAPK pathway. Therefore, AETC may be beneficial in lung carcinoma treatment.</abstract></addata></record> |
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source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; ScienceDirect Journals (5 years ago - present); Alma/SFX Local Collection |
subjects | A549细胞 体内实验 体外 水提取物 皮下埋植 红豆杉 蛋白激酶途径 表皮生长因子受体 |
title | Aqueous extract of Taxus Chinensis (Pilger) Rehd inhibits lung carci- noma A549 cells through the epidermal growth factor receptor/mi- togen-activated protein kinase pathway in vitro and in vivo |
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