Cloning and Expression Analysis of MIo Gene from Pericallis hybrida B. Nord

Cloning and Expression Analysis of MIo Gene from Pericallis hybrida B. Nord

The full-length Mlo gene was obtained by reverse transcription polymerase chain reaction （RT-PCR） and RACE. The result of sequence analysis indicated that M/o gene from Pericallis hybrida B. Nord. contained about 1296bp open reading frame and encoded 431 amino acids. According to the comparison of t...

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Veröffentlicht in:东北农业大学学报：英文版 2014, Vol.21 (1), p.10-15
1. Verfasser: Wang Jin-gan Li Wei Ren Hong-wei Lv Yuan-da Bai Ding Xu Jing
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description The full-length Mlo gene was obtained by reverse transcription polymerase chain reaction （RT-PCR） and RACE. The result of sequence analysis indicated that M/o gene from Pericallis hybrida B. Nord. contained about 1296bp open reading frame and encoded 431 amino acids. According to the comparison of the exogenous gene sequences by BLAST analysis and phylogenetic analysis, Mlo gene shared over 85% nucleotide homology and 98% amino acid homology. Finally, through semi-quantitative-PCR and fluorescence quantitative analysis, we found that Mlo gene showed the highest expression levels in leaves and the lowest in roots after inoculated with powdery mildew pathogen for different days.
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title Cloning and Expression Analysis of MIo Gene from Pericallis hybrida B. Nord
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