Electrochemical Immuno-Vascular Solid State Point of Care Testing （POCT）
In this work, it is proposed a POCT and innovative method of immunoassay for the detection of C-reactive protein and IgG, using Amperometry coupled to solid state kit, connected to a micro-flow system with comparable sensitivity to a high sensitivity CRP ELISA （hsCRP） and IgG ELISA, with 1-3 min tur...
Gespeichert in:
| Veröffentlicht in: | 材料科学与工程：中英文B版 2013, Vol.3 (7), p.452-463 |
|---|---|
| 1. Verfasser: | |
| Format: | Artikel |
| Sprache: | chi |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 463 |
|---|---|
| container_issue | 7 |
| container_start_page | 452 |
| container_title | 材料科学与工程：中英文B版 |
| container_volume | 3 |
| creator | Harold E. Braustein Klementiy Levkov Isabella E. Braustein Yifat Bezalel Majeda Abo Zaid Gideon Fleminger Judith Rishpon |
| description | In this work, it is proposed a POCT and innovative method of immunoassay for the detection of C-reactive protein and IgG, using Amperometry coupled to solid state kit, connected to a micro-flow system with comparable sensitivity to a high sensitivity CRP ELISA （hsCRP） and IgG ELISA, with 1-3 min turnaround time to result. Samples of CRP （0 to 250 ng·mL-1） and IgG or diluted spiked human serum are injected through a solid state polymeric kit, micro-flow sensor channels of a SWCT SPE nano-modified biosensor. Preparing two kits immuno-assays, in the same micro-column, built on oxirane groups of polymeric bead surface, with biological support to CRP and IgG biomarkers recognition, in a real time scheme, at the end of analyte injection the initial rate of change in current intensity I/A was proportional to CRP respectively IgG concentration, with low detection limit （LOD） of 0.1 ng·mL-1. It was revealed that CRP/IgG concentrations in serum that might be expected in both normal and pathological conditions can be de |
| format | Article |
| fullrecord | <record><control><sourceid>chongqing</sourceid><recordid>TN_cdi_chongqing_primary_48143900</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><cqvip_id>48143900</cqvip_id><sourcerecordid>48143900</sourcerecordid><originalsourceid>FETCH-chongqing_primary_481439003</originalsourceid><addsrcrecordid>eNpjYeA0MjQz1DUzMjLkYOAtLs5MMjA0NDE1NDG25GTwcc1JTS4pyk_OSM3NTE7MUfDMzS3Ny9cNSyxOLs1JLFIIzs_JTFEILkksSVUIyM_MK1HIT1NwTixKVQhJLS7JzEtXeL-nI8DfOeT9nk4eBta0xJziVF4ozc2g6OYa4uyhm5yRn5deCFQcX1CUmZtYVBlvYgGy38DAmBg1APTPPc4</addsrcrecordid><sourcetype>Publisher</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Electrochemical Immuno-Vascular Solid State Point of Care Testing （POCT）</title><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><creator>Harold E. Braustein Klementiy Levkov Isabella E. Braustein Yifat Bezalel Majeda Abo Zaid Gideon Fleminger Judith Rishpon</creator><creatorcontrib>Harold E. Braustein Klementiy Levkov Isabella E. Braustein Yifat Bezalel Majeda Abo Zaid Gideon Fleminger Judith Rishpon</creatorcontrib><description>In this work, it is proposed a POCT and innovative method of immunoassay for the detection of C-reactive protein and IgG, using Amperometry coupled to solid state kit, connected to a micro-flow system with comparable sensitivity to a high sensitivity CRP ELISA （hsCRP） and IgG ELISA, with 1-3 min turnaround time to result. Samples of CRP （0 to 250 ng·mL-1） and IgG or diluted spiked human serum are injected through a solid state polymeric kit, micro-flow sensor channels of a SWCT SPE nano-modified biosensor. Preparing two kits immuno-assays, in the same micro-column, built on oxirane groups of polymeric bead surface, with biological support to CRP and IgG biomarkers recognition, in a real time scheme, at the end of analyte injection the initial rate of change in current intensity I/A was proportional to CRP respectively IgG concentration, with low detection limit （LOD） of 0.1 ng·mL-1. It was revealed that CRP/IgG concentrations in serum that might be expected in both normal and pathological conditions can be de</description><identifier>ISSN: 2161-6221</identifier><language>chi</language><subject>C反应蛋白 ; ELISA ; 免疫分析 ; 固态聚合 ; 检测试剂盒 ; 检测限 ; 生物传感器 ; 电化学</subject><ispartof>材料科学与工程：中英文B版, 2013, Vol.3 (7), p.452-463</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/71213X/71213X.jpg</thumbnail><link.rule.ids>314,776,780,4010</link.rule.ids></links><search><creatorcontrib>Harold E. Braustein Klementiy Levkov Isabella E. Braustein Yifat Bezalel Majeda Abo Zaid Gideon Fleminger Judith Rishpon</creatorcontrib><title>Electrochemical Immuno-Vascular Solid State Point of Care Testing （POCT）</title><title>材料科学与工程：中英文B版</title><addtitle>Journal of Materials Science and Engineering B</addtitle><description>In this work, it is proposed a POCT and innovative method of immunoassay for the detection of C-reactive protein and IgG, using Amperometry coupled to solid state kit, connected to a micro-flow system with comparable sensitivity to a high sensitivity CRP ELISA （hsCRP） and IgG ELISA, with 1-3 min turnaround time to result. Samples of CRP （0 to 250 ng·mL-1） and IgG or diluted spiked human serum are injected through a solid state polymeric kit, micro-flow sensor channels of a SWCT SPE nano-modified biosensor. Preparing two kits immuno-assays, in the same micro-column, built on oxirane groups of polymeric bead surface, with biological support to CRP and IgG biomarkers recognition, in a real time scheme, at the end of analyte injection the initial rate of change in current intensity I/A was proportional to CRP respectively IgG concentration, with low detection limit （LOD） of 0.1 ng·mL-1. It was revealed that CRP/IgG concentrations in serum that might be expected in both normal and pathological conditions can be de</description><subject>C反应蛋白</subject><subject>ELISA</subject><subject>免疫分析</subject><subject>固态聚合</subject><subject>检测试剂盒</subject><subject>检测限</subject><subject>生物传感器</subject><subject>电化学</subject><issn>2161-6221</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><recordid>eNpjYeA0MjQz1DUzMjLkYOAtLs5MMjA0NDE1NDG25GTwcc1JTS4pyk_OSM3NTE7MUfDMzS3Ny9cNSyxOLs1JLFIIzs_JTFEILkksSVUIyM_MK1HIT1NwTixKVQhJLS7JzEtXeL-nI8DfOeT9nk4eBta0xJziVF4ozc2g6OYa4uyhm5yRn5deCFQcX1CUmZtYVBlvYgGy38DAmBg1APTPPc4</recordid><startdate>2013</startdate><enddate>2013</enddate><creator>Harold E. Braustein Klementiy Levkov Isabella E. Braustein Yifat Bezalel Majeda Abo Zaid Gideon Fleminger Judith Rishpon</creator><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W92</scope><scope>~WA</scope></search><sort><creationdate>2013</creationdate><title>Electrochemical Immuno-Vascular Solid State Point of Care Testing （POCT）</title><author>Harold E. Braustein Klementiy Levkov Isabella E. Braustein Yifat Bezalel Majeda Abo Zaid Gideon Fleminger Judith Rishpon</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-chongqing_primary_481439003</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi</language><creationdate>2013</creationdate><topic>C反应蛋白</topic><topic>ELISA</topic><topic>免疫分析</topic><topic>固态聚合</topic><topic>检测试剂盒</topic><topic>检测限</topic><topic>生物传感器</topic><topic>电化学</topic><toplevel>online_resources</toplevel><creatorcontrib>Harold E. Braustein Klementiy Levkov Isabella E. Braustein Yifat Bezalel Majeda Abo Zaid Gideon Fleminger Judith Rishpon</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-工程技术</collection><collection>中文科技期刊数据库- 镜像站点</collection><jtitle>材料科学与工程：中英文B版</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Harold E. Braustein Klementiy Levkov Isabella E. Braustein Yifat Bezalel Majeda Abo Zaid Gideon Fleminger Judith Rishpon</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Electrochemical Immuno-Vascular Solid State Point of Care Testing （POCT）</atitle><jtitle>材料科学与工程：中英文B版</jtitle><addtitle>Journal of Materials Science and Engineering B</addtitle><date>2013</date><risdate>2013</risdate><volume>3</volume><issue>7</issue><spage>452</spage><epage>463</epage><pages>452-463</pages><issn>2161-6221</issn><abstract>In this work, it is proposed a POCT and innovative method of immunoassay for the detection of C-reactive protein and IgG, using Amperometry coupled to solid state kit, connected to a micro-flow system with comparable sensitivity to a high sensitivity CRP ELISA （hsCRP） and IgG ELISA, with 1-3 min turnaround time to result. Samples of CRP （0 to 250 ng·mL-1） and IgG or diluted spiked human serum are injected through a solid state polymeric kit, micro-flow sensor channels of a SWCT SPE nano-modified biosensor. Preparing two kits immuno-assays, in the same micro-column, built on oxirane groups of polymeric bead surface, with biological support to CRP and IgG biomarkers recognition, in a real time scheme, at the end of analyte injection the initial rate of change in current intensity I/A was proportional to CRP respectively IgG concentration, with low detection limit （LOD） of 0.1 ng·mL-1. It was revealed that CRP/IgG concentrations in serum that might be expected in both normal and pathological conditions can be de</abstract></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2161-6221 |
| ispartof | 材料科学与工程：中英文B版, 2013, Vol.3 (7), p.452-463 |
| issn | 2161-6221 |
| language | chi |
| recordid | cdi_chongqing_primary_48143900 |
| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | C反应蛋白 ELISA 免疫分析 固态聚合 检测试剂盒 检测限 生物传感器 电化学 |
| title | Electrochemical Immuno-Vascular Solid State Point of Care Testing （POCT） |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-06T12%3A30%3A15IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-chongqing&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Electrochemical%20Immuno-Vascular%20Solid%20State%20Point%20of%20Care%20Testing%20%EF%BC%88POCT%EF%BC%89&rft.jtitle=%E6%9D%90%E6%96%99%E7%A7%91%E5%AD%A6%E4%B8%8E%E5%B7%A5%E7%A8%8B%EF%BC%9A%E4%B8%AD%E8%8B%B1%E6%96%87B%E7%89%88&rft.au=Harold%20E.%20Braustein%20Klementiy%20Levkov%20Isabella%20E.%20Braustein%20Yifat%20Bezalel%20Majeda%20Abo%20Zaid%20Gideon%20Fleminger%20Judith%20Rishpon&rft.date=2013&rft.volume=3&rft.issue=7&rft.spage=452&rft.epage=463&rft.pages=452-463&rft.issn=2161-6221&rft_id=info:doi/&rft_dat=%3Cchongqing%3E48143900%3C/chongqing%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rft_cqvip_id=48143900&rfr_iscdi=true |