Effect of kidney-reinforcing, blood-activating and stasis-removing recipes on adhesion molecule expression of bone marrow mesen- chymal stem cells from chronic aplastic anemia patients

OBJECTIVE: To explore the effect of kidney-rein- forcing, blood-activating and stasis-removing recipes on adhesion molecule expression of bone mar- row mesenchymal stem cells (MSCs) from patients with chronic aplastic anemia (CAA). METHODS: We used three Traditional Chinese Medicine recipes, namely...

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Veröffentlicht in:中医杂志:英文版 2012, Vol.32 (4), p.596-603
1. Verfasser: Baodong Ye Dan Chen Dijiong Wu Xiangping Wu Xiang Zhang Yuhong Zhou Yiping Shen Keding Shao Qin-hong Yu
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description OBJECTIVE: To explore the effect of kidney-rein- forcing, blood-activating and stasis-removing recipes on adhesion molecule expression of bone mar- row mesenchymal stem cells (MSCs) from patients with chronic aplastic anemia (CAA). METHODS: We used three Traditional Chinese Medicine recipes, namely a kidney-reinforcing recipe (KRR), blood-activating and stasis-removing recipe (BASRR), and kidney-reinforcing, blood-activating and stasis-removing recipe (KRBASFIR), and a nor- mal saline control to prepare herbal medicine se- rum in Sprague Dawley rats. Thirty CAA patients were enrolled in the experimental group, including 17 kidney-Yang deficient patients and 13 kidney-Yin deficient patients. Ten healthy individuals were included in the control group. MSCs were isolated from bone marrow samples, and the cell density was observed to measure their proliferation ability by microscopy on days 2, 7, and 14 after isolation. In addition, the expression of adhesion molecules of bone marrow MSCs (CD106, CD49d, CD31 and CD44) were detected by flow cytometry after 48 h of treatment with the four different herbal medi- cine serums. RESULTS: The proliferation of MSCs from kid- ney-Yang deficient and kidney-Yin deficient pa- tients was weaker than that of MSCs from the con- trol group. The expression of all adhesion mole- cules of bone marrow MSCs from CAA patients was obviously lower than that in the control group (P〈 0.01). The expression of CD49d and CD31 in MSCs from patients with a kidney-Yin deficiency was low- er than in those with a kidney-yang deficiency (P〈 0.05 and P〈O.01, respectively). For kidney-Yang defi- cient patients, CD31 expression in the KRBASRR group was significantly higher than that in the BASRR group (P〈O.01), while CD44 in the KRBASRR group was significantly higher than that in both KRR and BASRR groups (P〈O.01). For kidney-Yin defi- cient patients, CD106 and CD49d expression in the KRBASRR group was obviously higher than that in the KRR group (P〈0.05), while CD31 and CD44 ex- pression in the KRBASRR group was significantly higher than that in both KRR and BASRR groups (P〈 0.05 and P〈0.01, respectively). CONCLUSION: The bone marrow microenviron- ment in CAA patients is abnormal. The effect of KRBASRR may be better than that of KRR and BASRR for kidney-Yang deficient and kidney-Yin de- ficient patients by improving the expression levels of MSC adhesion molecules.
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METHODS: We used three Traditional Chinese Medicine recipes, namely a kidney-reinforcing recipe (KRR), blood-activating and stasis-removing recipe (BASRR), and kidney-reinforcing, blood-activating and stasis-removing recipe (KRBASFIR), and a nor- mal saline control to prepare herbal medicine se- rum in Sprague Dawley rats. Thirty CAA patients were enrolled in the experimental group, including 17 kidney-Yang deficient patients and 13 kidney-Yin deficient patients. Ten healthy individuals were included in the control group. MSCs were isolated from bone marrow samples, and the cell density was observed to measure their proliferation ability by microscopy on days 2, 7, and 14 after isolation. In addition, the expression of adhesion molecules of bone marrow MSCs (CD106, CD49d, CD31 and CD44) were detected by flow cytometry after 48 h of treatment with the four different herbal medi- cine serums. RESULTS: The proliferation of MSCs from kid- ney-Yang deficient and kidney-Yin deficient pa- tients was weaker than that of MSCs from the con- trol group. The expression of all adhesion mole- cules of bone marrow MSCs from CAA patients was obviously lower than that in the control group (P〈 0.01). The expression of CD49d and CD31 in MSCs from patients with a kidney-Yin deficiency was low- er than in those with a kidney-yang deficiency (P〈 0.05 and P〈O.01, respectively). For kidney-Yang defi- cient patients, CD31 expression in the KRBASRR group was significantly higher than that in the BASRR group (P〈O.01), while CD44 in the KRBASRR group was significantly higher than that in both KRR and BASRR groups (P〈O.01). For kidney-Yin defi- cient patients, CD106 and CD49d expression in the KRBASRR group was obviously higher than that in the KRR group (P〈0.05), while CD31 and CD44 ex- pression in the KRBASRR group was significantly higher than that in both KRR and BASRR groups (P〈 0.05 and P〈0.01, respectively). CONCLUSION: The bone marrow microenviron- ment in CAA patients is abnormal. The effect of KRBASRR may be better than that of KRR and BASRR for kidney-Yang deficient and kidney-Yin de- ficient patients by improving the expression levels of MSC adhesion molecules.</description><identifier>ISSN: 0255-2922</identifier><language>eng</language><subject>再生障碍性贫血 ; 患者 ; 慢性 ; 活血 ; 细胞黏附分子 ; 肾阳虚 ; 食谱 ; 骨髓间充质干细胞</subject><ispartof>中医杂志:英文版, 2012, Vol.32 (4), p.596-603</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/86801X/86801X.jpg</thumbnail><link.rule.ids>314,778,782,4012</link.rule.ids></links><search><creatorcontrib>Baodong Ye Dan Chen Dijiong Wu Xiangping Wu Xiang Zhang Yuhong Zhou Yiping Shen Keding Shao Qin-hong Yu</creatorcontrib><title>Effect of kidney-reinforcing, blood-activating and stasis-removing recipes on adhesion molecule expression of bone marrow mesen- chymal stem cells from chronic aplastic anemia patients</title><title>中医杂志:英文版</title><addtitle>Journal of Traditional Chinese Medicine</addtitle><description>OBJECTIVE: To explore the effect of kidney-rein- forcing, blood-activating and stasis-removing recipes on adhesion molecule expression of bone mar- row mesenchymal stem cells (MSCs) from patients with chronic aplastic anemia (CAA). METHODS: We used three Traditional Chinese Medicine recipes, namely a kidney-reinforcing recipe (KRR), blood-activating and stasis-removing recipe (BASRR), and kidney-reinforcing, blood-activating and stasis-removing recipe (KRBASFIR), and a nor- mal saline control to prepare herbal medicine se- rum in Sprague Dawley rats. Thirty CAA patients were enrolled in the experimental group, including 17 kidney-Yang deficient patients and 13 kidney-Yin deficient patients. Ten healthy individuals were included in the control group. MSCs were isolated from bone marrow samples, and the cell density was observed to measure their proliferation ability by microscopy on days 2, 7, and 14 after isolation. In addition, the expression of adhesion molecules of bone marrow MSCs (CD106, CD49d, CD31 and CD44) were detected by flow cytometry after 48 h of treatment with the four different herbal medi- cine serums. RESULTS: The proliferation of MSCs from kid- ney-Yang deficient and kidney-Yin deficient pa- tients was weaker than that of MSCs from the con- trol group. The expression of all adhesion mole- cules of bone marrow MSCs from CAA patients was obviously lower than that in the control group (P〈 0.01). The expression of CD49d and CD31 in MSCs from patients with a kidney-Yin deficiency was low- er than in those with a kidney-yang deficiency (P〈 0.05 and P〈O.01, respectively). For kidney-Yang defi- cient patients, CD31 expression in the KRBASRR group was significantly higher than that in the BASRR group (P〈O.01), while CD44 in the KRBASRR group was significantly higher than that in both KRR and BASRR groups (P〈O.01). For kidney-Yin defi- cient patients, CD106 and CD49d expression in the KRBASRR group was obviously higher than that in the KRR group (P〈0.05), while CD31 and CD44 ex- pression in the KRBASRR group was significantly higher than that in both KRR and BASRR groups (P〈 0.05 and P〈0.01, respectively). CONCLUSION: The bone marrow microenviron- ment in CAA patients is abnormal. The effect of KRBASRR may be better than that of KRR and BASRR for kidney-Yang deficient and kidney-Yin de- ficient patients by improving the expression levels of MSC adhesion molecules.</description><subject>再生障碍性贫血</subject><subject>患者</subject><subject>慢性</subject><subject>活血</subject><subject>细胞黏附分子</subject><subject>肾阳虚</subject><subject>食谱</subject><subject>骨髓间充质干细胞</subject><issn>0255-2922</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNqNjk1OAzEMhbMAiUK5g9kz0mh-qrJGRRyAfeVmnE4gsUMcCnMzjkeKOAArf_r05PcuzKrtxrHpHrruylyrvrbtuB3H7cp875wjW0AcvPmJaWkyeXaSrefjPRyCyNSgLf6EpRpAnkALqtcajHI6u0zWJ1IQBpxmUl8hSiD7EQjoK2XSX1c7DsIEEXOWT4ikxA3YeYkY6lOKYCkEBZel4pyFvQVMAbWcgSl6hFR3EBddm0uHQen2796Yu6fdy-NzY2fh43vdtU_Z16plPwx912-GTf-fzA8u7GT-</recordid><startdate>2012</startdate><enddate>2012</enddate><creator>Baodong Ye Dan Chen Dijiong Wu Xiangping Wu Xiang Zhang Yuhong Zhou Yiping Shen Keding Shao Qin-hong Yu</creator><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope></search><sort><creationdate>2012</creationdate><title>Effect of kidney-reinforcing, blood-activating and stasis-removing recipes on adhesion molecule expression of bone marrow mesen- chymal stem cells from chronic aplastic anemia patients</title><author>Baodong Ye Dan Chen Dijiong Wu Xiangping Wu Xiang Zhang Yuhong Zhou Yiping Shen Keding Shao Qin-hong Yu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-chongqing_primary_443236463</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>再生障碍性贫血</topic><topic>患者</topic><topic>慢性</topic><topic>活血</topic><topic>细胞黏附分子</topic><topic>肾阳虚</topic><topic>食谱</topic><topic>骨髓间充质干细胞</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Baodong Ye Dan Chen Dijiong Wu Xiangping Wu Xiang Zhang Yuhong Zhou Yiping Shen Keding Shao Qin-hong Yu</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><jtitle>中医杂志:英文版</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Baodong Ye Dan Chen Dijiong Wu Xiangping Wu Xiang Zhang Yuhong Zhou Yiping Shen Keding Shao Qin-hong Yu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of kidney-reinforcing, blood-activating and stasis-removing recipes on adhesion molecule expression of bone marrow mesen- chymal stem cells from chronic aplastic anemia patients</atitle><jtitle>中医杂志:英文版</jtitle><addtitle>Journal of Traditional Chinese Medicine</addtitle><date>2012</date><risdate>2012</risdate><volume>32</volume><issue>4</issue><spage>596</spage><epage>603</epage><pages>596-603</pages><issn>0255-2922</issn><abstract>OBJECTIVE: To explore the effect of kidney-rein- forcing, blood-activating and stasis-removing recipes on adhesion molecule expression of bone mar- row mesenchymal stem cells (MSCs) from patients with chronic aplastic anemia (CAA). METHODS: We used three Traditional Chinese Medicine recipes, namely a kidney-reinforcing recipe (KRR), blood-activating and stasis-removing recipe (BASRR), and kidney-reinforcing, blood-activating and stasis-removing recipe (KRBASFIR), and a nor- mal saline control to prepare herbal medicine se- rum in Sprague Dawley rats. Thirty CAA patients were enrolled in the experimental group, including 17 kidney-Yang deficient patients and 13 kidney-Yin deficient patients. Ten healthy individuals were included in the control group. MSCs were isolated from bone marrow samples, and the cell density was observed to measure their proliferation ability by microscopy on days 2, 7, and 14 after isolation. In addition, the expression of adhesion molecules of bone marrow MSCs (CD106, CD49d, CD31 and CD44) were detected by flow cytometry after 48 h of treatment with the four different herbal medi- cine serums. RESULTS: The proliferation of MSCs from kid- ney-Yang deficient and kidney-Yin deficient pa- tients was weaker than that of MSCs from the con- trol group. The expression of all adhesion mole- cules of bone marrow MSCs from CAA patients was obviously lower than that in the control group (P〈 0.01). The expression of CD49d and CD31 in MSCs from patients with a kidney-Yin deficiency was low- er than in those with a kidney-yang deficiency (P〈 0.05 and P〈O.01, respectively). For kidney-Yang defi- cient patients, CD31 expression in the KRBASRR group was significantly higher than that in the BASRR group (P〈O.01), while CD44 in the KRBASRR group was significantly higher than that in both KRR and BASRR groups (P〈O.01). For kidney-Yin defi- cient patients, CD106 and CD49d expression in the KRBASRR group was obviously higher than that in the KRR group (P〈0.05), while CD31 and CD44 ex- pression in the KRBASRR group was significantly higher than that in both KRR and BASRR groups (P〈 0.05 and P〈0.01, respectively). CONCLUSION: The bone marrow microenviron- ment in CAA patients is abnormal. The effect of KRBASRR may be better than that of KRR and BASRR for kidney-Yang deficient and kidney-Yin de- ficient patients by improving the expression levels of MSC adhesion molecules.</abstract></addata></record>
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subjects 再生障碍性贫血
患者
慢性
活血
细胞黏附分子
肾阳虚
食谱
骨髓间充质干细胞
title Effect of kidney-reinforcing, blood-activating and stasis-removing recipes on adhesion molecule expression of bone marrow mesen- chymal stem cells from chronic aplastic anemia patients
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