Effect of Yiguanjian decoction on cell differentiation and proliferation in CCI4-treated mice

AIM: To investigate the cellular mechanisms of action of Yiguanjian (YGJ) decoction in treatment of chronic hepatic injury. METHODS: One group of mice was irradiated, and received enhanced green fluorescent protein (EGFP)- positive bone marrow transplants followed by 13 wk of CCh injection and 6 wk...

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Veröffentlicht in:世界胃肠病学杂志:英文版 2012, Vol.18 (25), p.3235-3249
1. Verfasser: Xiao-Ling Wang Dong-Wei Jia Hui-Yang Liu Xiao-Feng Yan Ting-Jie Ye Xu-Dong Hu Bo-Qin Li Yong-Liang Chen Ping Liu
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description AIM: To investigate the cellular mechanisms of action of Yiguanjian (YGJ) decoction in treatment of chronic hepatic injury. METHODS: One group of mice was irradiated, and received enhanced green fluorescent protein (EGFP)- positive bone marrow transplants followed by 13 wk of CCh injection and 6 wk of oral YGJ administration. A second group of Institute for Cancer Research mice was treated with 13 wk of CCI4 injection and 6 wk of oral YGJadministration. Liver function, histological changes in the liver, and Hyp content were analyzed. The expres- sion of m-smooth muscle actin (α-SMA), F4/80, albumin (AIb), EGFP, mitogen-activated protein kinase-2 (PKM2), Ki-67, fetoprotein (AFP), monocyte chemotaxis pro- tein-1 and CC chemokine receptor 2 were assayed. RESULTS: As hepatic damage progressed, EGFP-po- sitive marrow cells migrated into the liver and were mainly distributed along the fibrous septa. They showed a conspicuous coexpression of EGFP with ~-SMA and F4/80 but no coexpression with AIb. Moreover, the expression of PKM2, AFP and Ki-67 was enhanced dy- namically and steadily over the course of liver injury. YGJ abrogated the increases in the number of bone marrow-derived fibrogenic cells in the liver, inhibited expression of both progenitor and mature hepatocyte markers, and reduced fibrogenesis. CONCLUSION: YGJ decoction improves liver fibrosis by inhibiting the migration of bone marrow cells into the liver as well as inhibiting their differentiation and suppressing the proliferation of both progenitors and hepatocytes in the injured liver.
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METHODS: One group of mice was irradiated, and received enhanced green fluorescent protein (EGFP)- positive bone marrow transplants followed by 13 wk of CCh injection and 6 wk of oral YGJ administration. A second group of Institute for Cancer Research mice was treated with 13 wk of CCI4 injection and 6 wk of oral YGJadministration. Liver function, histological changes in the liver, and Hyp content were analyzed. The expres- sion of m-smooth muscle actin (α-SMA), F4/80, albumin (AIb), EGFP, mitogen-activated protein kinase-2 (PKM2), Ki-67, fetoprotein (AFP), monocyte chemotaxis pro- tein-1 and CC chemokine receptor 2 were assayed. RESULTS: As hepatic damage progressed, EGFP-po- sitive marrow cells migrated into the liver and were mainly distributed along the fibrous septa. They showed a conspicuous coexpression of EGFP with ~-SMA and F4/80 but no coexpression with AIb. Moreover, the expression of PKM2, AFP and Ki-67 was enhanced dy- namically and steadily over the course of liver injury. YGJ abrogated the increases in the number of bone marrow-derived fibrogenic cells in the liver, inhibited expression of both progenitor and mature hepatocyte markers, and reduced fibrogenesis. CONCLUSION: YGJ decoction improves liver fibrosis by inhibiting the migration of bone marrow cells into the liver as well as inhibiting their differentiation and suppressing the proliferation of both progenitors and hepatocytes in the injured liver.</description><identifier>ISSN: 1007-9327</identifier><identifier>EISSN: 2219-2840</identifier><language>eng</language><subject>一贯煎 ; 丝裂原活化蛋白激酶 ; 增强型绿色荧光蛋白 ; 增殖 ; 小鼠 ; 治疗 ; 煎剂 ; 细胞分化</subject><ispartof>世界胃肠病学杂志:英文版, 2012, Vol.18 (25), p.3235-3249</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/84123X/84123X.jpg</thumbnail><link.rule.ids>314,780,784,4024</link.rule.ids></links><search><creatorcontrib>Xiao-Ling Wang Dong-Wei Jia Hui-Yang Liu Xiao-Feng Yan Ting-Jie Ye Xu-Dong Hu Bo-Qin Li Yong-Liang Chen Ping Liu</creatorcontrib><title>Effect of Yiguanjian decoction on cell differentiation and proliferation in CCI4-treated mice</title><title>世界胃肠病学杂志:英文版</title><addtitle>World Journal of Gastroenterology</addtitle><description>AIM: To investigate the cellular mechanisms of action of Yiguanjian (YGJ) decoction in treatment of chronic hepatic injury. METHODS: One group of mice was irradiated, and received enhanced green fluorescent protein (EGFP)- positive bone marrow transplants followed by 13 wk of CCh injection and 6 wk of oral YGJ administration. A second group of Institute for Cancer Research mice was treated with 13 wk of CCI4 injection and 6 wk of oral YGJadministration. Liver function, histological changes in the liver, and Hyp content were analyzed. The expres- sion of m-smooth muscle actin (α-SMA), F4/80, albumin (AIb), EGFP, mitogen-activated protein kinase-2 (PKM2), Ki-67, fetoprotein (AFP), monocyte chemotaxis pro- tein-1 and CC chemokine receptor 2 were assayed. RESULTS: As hepatic damage progressed, EGFP-po- sitive marrow cells migrated into the liver and were mainly distributed along the fibrous septa. They showed a conspicuous coexpression of EGFP with ~-SMA and F4/80 but no coexpression with AIb. Moreover, the expression of PKM2, AFP and Ki-67 was enhanced dy- namically and steadily over the course of liver injury. YGJ abrogated the increases in the number of bone marrow-derived fibrogenic cells in the liver, inhibited expression of both progenitor and mature hepatocyte markers, and reduced fibrogenesis. 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Moreover, the expression of PKM2, AFP and Ki-67 was enhanced dy- namically and steadily over the course of liver injury. YGJ abrogated the increases in the number of bone marrow-derived fibrogenic cells in the liver, inhibited expression of both progenitor and mature hepatocyte markers, and reduced fibrogenesis. CONCLUSION: YGJ decoction improves liver fibrosis by inhibiting the migration of bone marrow cells into the liver as well as inhibiting their differentiation and suppressing the proliferation of both progenitors and hepatocytes in the injured liver.</abstract></addata></record>
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subjects 一贯煎
丝裂原活化蛋白激酶
增强型绿色荧光蛋白
增殖
小鼠
治疗
煎剂
细胞分化
title Effect of Yiguanjian decoction on cell differentiation and proliferation in CCI4-treated mice
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