A Microarray Analysis of Angiogenesis Modulation Effect of Xuefu Zhuyu Decoction （血府逐瘀汤） on Endothelial Cells
Objective： To study the angiogenesis modulation mechanism of Xuefu Zhuyu Decoction （血府逐瘀汤） on the endothelial cell line ECV304. Methods： ECV304 cells were treated with 2.5% Xuefu Zhuyu Decoction- containing serum （XFZYD-CS） for 24 h, 48 h or 72 h. Thiazolyl blue tetrazolium bromide （MTT）, fluorescen...
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| Veröffentlicht in: | 中国结合医学杂志：英文版 2012, Vol.18 (7), p.502-506 |
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| container_title | 中国结合医学杂志：英文版 |
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| creator | SONG Jun CHEN Wen-yuan WU Li-ya ZHENG Liang-pu LIN Wei GAO Dong Ted J. Kaptchuk CHEN Ke-ji |
| description | Objective： To study the angiogenesis modulation mechanism of Xuefu Zhuyu Decoction （血府逐瘀汤） on the endothelial cell line ECV304. Methods： ECV304 cells were treated with 2.5% Xuefu Zhuyu Decoction- containing serum （XFZYD-CS） for 24 h, 48 h or 72 h. Thiazolyl blue tetrazolium bromide （MTT）, fluorescence activating cell sorter （FACS）, migration, adhesion and in vitro tube formation assays were conducted to confirm an angiogenesis effect of XFZYD at 3 time points. An analysis of angiogenesis regulator profiles was performed at 3 times with real-time polymerase chain reaction （RT-PCR） superarray. Results： At 48 h, XFZYD-CS induced ECV304 significantly improved cell viability, number in S phase, migration, adhesion and tube formation. At 24 h and 72 h, only cell migration was elevated. Microarray results showed that the expression of 27 angiogenesis-related genes was changed. Conclusion： XFZYD-CS treatment induced angiogenesis on ECV304 cells with significant cellcular changes occurring at 48 h and genetic changes as early as 24 h. |
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Kaptchuk CHEN Ke-ji</creator><creatorcontrib>SONG Jun CHEN Wen-yuan WU Li-ya ZHENG Liang-pu LIN Wei GAO Dong Ted J. Kaptchuk CHEN Ke-ji</creatorcontrib><description>Objective： To study the angiogenesis modulation mechanism of Xuefu Zhuyu Decoction （血府逐瘀汤） on the endothelial cell line ECV304. Methods： ECV304 cells were treated with 2.5% Xuefu Zhuyu Decoction- containing serum （XFZYD-CS） for 24 h, 48 h or 72 h. Thiazolyl blue tetrazolium bromide （MTT）, fluorescence activating cell sorter （FACS）, migration, adhesion and in vitro tube formation assays were conducted to confirm an angiogenesis effect of XFZYD at 3 time points. An analysis of angiogenesis regulator profiles was performed at 3 times with real-time polymerase chain reaction （RT-PCR） superarray. Results： At 48 h, XFZYD-CS induced ECV304 significantly improved cell viability, number in S phase, migration, adhesion and tube formation. At 24 h and 72 h, only cell migration was elevated. Microarray results showed that the expression of 27 angiogenesis-related genes was changed. Conclusion： XFZYD-CS treatment induced angiogenesis on ECV304 cells with significant cellcular changes occurring at 48 h and genetic changes as early as 24 h.</description><identifier>ISSN: 1672-0415</identifier><identifier>EISSN: 1993-0402</identifier><language>eng</language><subject>RT-PCR ; 微阵列分析 ; 聚合酶链反应 ; 血府逐瘀汤 ; 血管内皮细胞 ; 血管新生 ; 血管生成 ; 调制效应</subject><ispartof>中国结合医学杂志：英文版, 2012, Vol.18 (7), p.502-506</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/86437A/86437A.jpg</thumbnail><link.rule.ids>314,776,780,4010</link.rule.ids></links><search><creatorcontrib>SONG Jun CHEN Wen-yuan WU Li-ya ZHENG Liang-pu LIN Wei GAO Dong Ted J. Kaptchuk CHEN Ke-ji</creatorcontrib><title>A Microarray Analysis of Angiogenesis Modulation Effect of Xuefu Zhuyu Decoction （血府逐瘀汤） on Endothelial Cells</title><title>中国结合医学杂志：英文版</title><addtitle>Chinese Journal of Integrative Medicine</addtitle><description>Objective： To study the angiogenesis modulation mechanism of Xuefu Zhuyu Decoction （血府逐瘀汤） on the endothelial cell line ECV304. Methods： ECV304 cells were treated with 2.5% Xuefu Zhuyu Decoction- containing serum （XFZYD-CS） for 24 h, 48 h or 72 h. Thiazolyl blue tetrazolium bromide （MTT）, fluorescence activating cell sorter （FACS）, migration, adhesion and in vitro tube formation assays were conducted to confirm an angiogenesis effect of XFZYD at 3 time points. An analysis of angiogenesis regulator profiles was performed at 3 times with real-time polymerase chain reaction （RT-PCR） superarray. Results： At 48 h, XFZYD-CS induced ECV304 significantly improved cell viability, number in S phase, migration, adhesion and tube formation. At 24 h and 72 h, only cell migration was elevated. Microarray results showed that the expression of 27 angiogenesis-related genes was changed. Conclusion： XFZYD-CS treatment induced angiogenesis on ECV304 cells with significant cellcular changes occurring at 48 h and genetic changes as early as 24 h.</description><subject>RT-PCR</subject><subject>微阵列分析</subject><subject>聚合酶链反应</subject><subject>血府逐瘀汤</subject><subject>血管内皮细胞</subject><subject>血管新生</subject><subject>血管生成</subject><subject>调制效应</subject><issn>1672-0415</issn><issn>1993-0402</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNpjYuA0tLQ01jUwMTBiAbLNzI2AbENTDgau4uIsAwNTczMDU06GakcF38zkovzEoqLESgXHvMScyuLMYoX8NCA7PTM_PTUvFcT3zU8pzUksyczPU3BNS0tNLgGpiChNTStViMoorSxVcElNzk8Gy7_f0_FiYcPTXXNeNkx4PqPh2cYl7_d0KoA05qXkl2Sk5mQm5ig4p-bkFPMwsKYl5hSn8kJpbgZFN9cQZw_d5Iz8vPTCzLz0-IKizNzEosp4EyNjI3NjcwNjYtQAAG4QVYE</recordid><startdate>2012</startdate><enddate>2012</enddate><creator>SONG Jun CHEN Wen-yuan WU Li-ya ZHENG Liang-pu LIN Wei GAO Dong Ted J. Kaptchuk CHEN Ke-ji</creator><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope></search><sort><creationdate>2012</creationdate><title>A Microarray Analysis of Angiogenesis Modulation Effect of Xuefu Zhuyu Decoction （血府逐瘀汤） on Endothelial Cells</title><author>SONG Jun CHEN Wen-yuan WU Li-ya ZHENG Liang-pu LIN Wei GAO Dong Ted J. Kaptchuk CHEN Ke-ji</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-chongqing_primary_423273703</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>RT-PCR</topic><topic>微阵列分析</topic><topic>聚合酶链反应</topic><topic>血府逐瘀汤</topic><topic>血管内皮细胞</topic><topic>血管新生</topic><topic>血管生成</topic><topic>调制效应</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SONG Jun CHEN Wen-yuan WU Li-ya ZHENG Liang-pu LIN Wei GAO Dong Ted J. Kaptchuk CHEN Ke-ji</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><jtitle>中国结合医学杂志：英文版</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SONG Jun CHEN Wen-yuan WU Li-ya ZHENG Liang-pu LIN Wei GAO Dong Ted J. Kaptchuk CHEN Ke-ji</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Microarray Analysis of Angiogenesis Modulation Effect of Xuefu Zhuyu Decoction （血府逐瘀汤） on Endothelial Cells</atitle><jtitle>中国结合医学杂志：英文版</jtitle><addtitle>Chinese Journal of Integrative Medicine</addtitle><date>2012</date><risdate>2012</risdate><volume>18</volume><issue>7</issue><spage>502</spage><epage>506</epage><pages>502-506</pages><issn>1672-0415</issn><eissn>1993-0402</eissn><abstract>Objective： To study the angiogenesis modulation mechanism of Xuefu Zhuyu Decoction （血府逐瘀汤） on the endothelial cell line ECV304. Methods： ECV304 cells were treated with 2.5% Xuefu Zhuyu Decoction- containing serum （XFZYD-CS） for 24 h, 48 h or 72 h. Thiazolyl blue tetrazolium bromide （MTT）, fluorescence activating cell sorter （FACS）, migration, adhesion and in vitro tube formation assays were conducted to confirm an angiogenesis effect of XFZYD at 3 time points. An analysis of angiogenesis regulator profiles was performed at 3 times with real-time polymerase chain reaction （RT-PCR） superarray. Results： At 48 h, XFZYD-CS induced ECV304 significantly improved cell viability, number in S phase, migration, adhesion and tube formation. At 24 h and 72 h, only cell migration was elevated. Microarray results showed that the expression of 27 angiogenesis-related genes was changed. Conclusion： XFZYD-CS treatment induced angiogenesis on ECV304 cells with significant cellcular changes occurring at 48 h and genetic changes as early as 24 h.</abstract></addata></record> |
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| ispartof | 中国结合医学杂志：英文版, 2012, Vol.18 (7), p.502-506 |
| issn | 1672-0415 1993-0402 |
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| source | Alma/SFX Local Collection; SpringerLink Journals - AutoHoldings |
| subjects | RT-PCR 微阵列分析 聚合酶链反应 血府逐瘀汤 血管内皮细胞 血管新生 血管生成 调制效应 |
| title | A Microarray Analysis of Angiogenesis Modulation Effect of Xuefu Zhuyu Decoction （血府逐瘀汤） on Endothelial Cells |
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