RE-ESTABLISHMENT OF SYMBIOSIS TO Anabaena-FREE Azolla
Symbiosis to Anabaena-free Azolla has been re-established by placing indusium containing Anabaena onto the Anabaena-free megasporocarps. Anabaena-free megasporocarps were obtained by removing the indusium and the apical membrane of the megaspore apparatus of normal Azolla. The recovery of symbiosis...
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| Veröffentlicht in: | 中国科学：化学英文版 1989 (5), p.551-559 |
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| container_title | 中国科学：化学英文版 |
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| creator | 林沧 刘中柱 郑德英 唐龙飞 渡边岩 |
| description | Symbiosis to Anabaena-free Azolla has been re-established by placing indusium containing Anabaena onto the Anabaena-free megasporocarps. Anabaena-free megasporocarps were obtained by removing the indusium and the apical membrane of the megaspore apparatus of normal Azolla. The recovery of symbiosis in artificially reconstituted Azolla-Anabaena association was confirmed using the scanning electron microscope, monoclonal antibody test (McAb-C16), and nitrogen fixation capacity （ARA）.Laboratory-grown Anabaena azollae cells inoculated subsequently to the decapitated megaspore apparatus were observed to have entered the leaf cavities of sporophyte, although some portions of Anabaena filaments were found outside the leaf cavity. Plants invaded by artificially inoculated Anabaena did not have sufficient N2-fixing activity to allow the growth in the N-free medium. |
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Anabaena-free megasporocarps were obtained by removing the indusium and the apical membrane of the megaspore apparatus of normal Azolla. The recovery of symbiosis in artificially reconstituted Azolla-Anabaena association was confirmed using the scanning electron microscope, monoclonal antibody test (McAb-C16), and nitrogen fixation capacity （ARA）.Laboratory-grown Anabaena azollae cells inoculated subsequently to the decapitated megaspore apparatus were observed to have entered the leaf cavities of sporophyte, although some portions of Anabaena filaments were found outside the leaf cavity. Plants invaded by artificially inoculated Anabaena did not have sufficient N2-fixing activity to allow the growth in the N-free medium.</description><identifier>ISSN: 1674-7291</identifier><identifier>EISSN: 1869-1870</identifier><language>eng</language><subject>Anabaena-free ; Azolla ; incomplete ; re-establishment ; symbiosis</subject><ispartof>中国科学：化学英文版, 1989 (5), p.551-559</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/60113X/60113X.jpg</thumbnail><link.rule.ids>314,780,784,4024</link.rule.ids></links><search><creatorcontrib>林沧 刘中柱 郑德英 唐龙飞 渡边岩</creatorcontrib><title>RE-ESTABLISHMENT OF SYMBIOSIS TO Anabaena-FREE Azolla</title><title>中国科学：化学英文版</title><addtitle>SCIENCE CHINA Chemistry</addtitle><description>Symbiosis to Anabaena-free Azolla has been re-established by placing indusium containing Anabaena onto the Anabaena-free megasporocarps. Anabaena-free megasporocarps were obtained by removing the indusium and the apical membrane of the megaspore apparatus of normal Azolla. The recovery of symbiosis in artificially reconstituted Azolla-Anabaena association was confirmed using the scanning electron microscope, monoclonal antibody test (McAb-C16), and nitrogen fixation capacity （ARA）.Laboratory-grown Anabaena azollae cells inoculated subsequently to the decapitated megaspore apparatus were observed to have entered the leaf cavities of sporophyte, although some portions of Anabaena filaments were found outside the leaf cavity. Plants invaded by artificially inoculated Anabaena did not have sufficient N2-fixing activity to allow the growth in the N-free medium.</description><subject>Anabaena-free</subject><subject>Azolla</subject><subject>incomplete</subject><subject>re-establishment</subject><subject>symbiosis</subject><issn>1674-7291</issn><issn>1869-1870</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><recordid>eNpjYuA0tDCz1DW0MDdgAbLNzE10zY0sDTkYeIuLswyAwNjYwMjclJPBNMhV1zU4xNHJxzPYw9fVL0TB300hONLXydM_2DNYIcRfwTEvMSkxNS9R1y3I1VXBsSo_JyeRh4E1LTGnOJUXSnMzKLu5hjh76CZn5OelF2bmpccXFGXmJhZVxhsbGBiamAMdY2xMnCoA58UyfA</recordid><startdate>1989</startdate><enddate>1989</enddate><creator>林沧 刘中柱 郑德英 唐龙飞 渡边岩</creator><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W94</scope><scope>~WA</scope></search><sort><creationdate>1989</creationdate><title>RE-ESTABLISHMENT OF SYMBIOSIS TO Anabaena-FREE Azolla</title><author>林沧 刘中柱 郑德英 唐龙飞 渡边岩</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-chongqing_primary_30014786933</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Anabaena-free</topic><topic>Azolla</topic><topic>incomplete</topic><topic>re-establishment</topic><topic>symbiosis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>林沧 刘中柱 郑德英 唐龙飞 渡边岩</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-自然科学</collection><collection>中文科技期刊数据库- 镜像站点</collection><jtitle>中国科学：化学英文版</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>林沧 刘中柱 郑德英 唐龙飞 渡边岩</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>RE-ESTABLISHMENT OF SYMBIOSIS TO Anabaena-FREE Azolla</atitle><jtitle>中国科学：化学英文版</jtitle><addtitle>SCIENCE CHINA Chemistry</addtitle><date>1989</date><risdate>1989</risdate><issue>5</issue><spage>551</spage><epage>559</epage><pages>551-559</pages><issn>1674-7291</issn><eissn>1869-1870</eissn><abstract>Symbiosis to Anabaena-free Azolla has been re-established by placing indusium containing Anabaena onto the Anabaena-free megasporocarps. Anabaena-free megasporocarps were obtained by removing the indusium and the apical membrane of the megaspore apparatus of normal Azolla. The recovery of symbiosis in artificially reconstituted Azolla-Anabaena association was confirmed using the scanning electron microscope, monoclonal antibody test (McAb-C16), and nitrogen fixation capacity （ARA）.Laboratory-grown Anabaena azollae cells inoculated subsequently to the decapitated megaspore apparatus were observed to have entered the leaf cavities of sporophyte, although some portions of Anabaena filaments were found outside the leaf cavity. Plants invaded by artificially inoculated Anabaena did not have sufficient N2-fixing activity to allow the growth in the N-free medium.</abstract></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1674-7291 |
| ispartof | 中国科学：化学英文版, 1989 (5), p.551-559 |
| issn | 1674-7291 1869-1870 |
| language | eng |
| recordid | cdi_chongqing_primary_3001478693 |
| source | Alma/SFX Local Collection |
| subjects | Anabaena-free Azolla incomplete re-establishment symbiosis |
| title | RE-ESTABLISHMENT OF SYMBIOSIS TO Anabaena-FREE Azolla |
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