Molecular cloning of a cDNA related to vernalization(verc203) in winter wheat
A cDNA clone related to the vernalization in winter wheat(verc203)was harvested from the en-riched cold-induced cDNA library of 10~4 pfu with differential screening.The insert of verc203 in λ gt10 vector wassubcloned into the sites between BamH Ⅰ and Hind Ⅲ in pUC19 plasmid after being amplified wit...
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| Veröffentlicht in: | 中国科学：化学英文版 1995 (7), p.799-806 |
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| creator | 种康 谭克辉 黄华梁 梁厚果 |
| description | A cDNA clone related to the vernalization in winter wheat(verc203)was harvested from the en-riched cold-induced cDNA library of 10~4 pfu with differential screening.The insert of verc203 in λ gt10 vector wassubcloned into the sites between BamH Ⅰ and Hind Ⅲ in pUC19 plasmid after being amplified with PCR.the analysis of the Northern blotting with a probe of verc203 indicated that the verc203 has a negative signalfor the control and the devernalized mRNA and a positive signal for the vernalized winter wheat and non-vernalized spring wheat at about 2.6 kb. |
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cloning ; gene ; molecular ; vernalization ; wheat ; winter</subject><ispartof>中国科学：化学英文版, 1995 (7), p.799-806</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/60113X/60113X.jpg</thumbnail><link.rule.ids>314,780,784,4024</link.rule.ids></links><search><creatorcontrib>种康 谭克辉 黄华梁 梁厚果</creatorcontrib><title>Molecular cloning of a cDNA related to vernalization(verc203) in winter wheat</title><title>中国科学：化学英文版</title><addtitle>SCIENCE CHINA Chemistry</addtitle><description>A cDNA clone related to the vernalization in winter wheat(verc203)was harvested from the en-riched cold-induced cDNA library of 10~4 pfu with differential screening.The insert of verc203 in λ gt10 vector wassubcloned into the sites between BamH Ⅰ and Hind Ⅲ in pUC19 plasmid after being amplified with PCR.the analysis of the Northern blotting with a probe of verc203 indicated that the verc203 has a negative signalfor the control and the devernalized mRNA and a positive signal for the vernalized winter wheat and non-vernalized spring wheat at about 2.6 kb.</description><subject>cDNA</subject><subject>cloning</subject><subject>gene</subject><subject>molecular</subject><subject>vernalization</subject><subject>wheat</subject><subject>winter</subject><issn>1674-7291</issn><issn>1869-1870</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><recordid>eNqNjLsKwjAYhYMoWLTv8IOLDoVctGlH8YKLTu4lxLSNxETTaNGnN4MP4FnOd-DjDFBCirzMSMHxMHLOlxmnJRmjtOuuOIYxTPkqQcejM0o-jfAgjbPaNuBqECC3pzV4ZURQFwgOXspbYfRHBO3sPC5JMVuAttBrG5SHvlUiTNGoFqZT6a8naLbfnTeHTLbONo_4Xt29vgn_rhjGhHKeM8z-s758ID41</recordid><startdate>1995</startdate><enddate>1995</enddate><creator>种康 谭克辉 黄华梁 梁厚果</creator><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W94</scope><scope>~WA</scope></search><sort><creationdate>1995</creationdate><title>Molecular cloning of a cDNA related to vernalization(verc203) in winter wheat</title><author>种康 谭克辉 黄华梁 梁厚果</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-chongqing_primary_30012776303</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>cDNA</topic><topic>cloning</topic><topic>gene</topic><topic>molecular</topic><topic>vernalization</topic><topic>wheat</topic><topic>winter</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>种康 谭克辉 黄华梁 梁厚果</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-自然科学</collection><collection>中文科技期刊数据库- 镜像站点</collection><jtitle>中国科学：化学英文版</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>种康 谭克辉 黄华梁 梁厚果</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular cloning of a cDNA related to vernalization(verc203) in winter wheat</atitle><jtitle>中国科学：化学英文版</jtitle><addtitle>SCIENCE CHINA Chemistry</addtitle><date>1995</date><risdate>1995</risdate><issue>7</issue><spage>799</spage><epage>806</epage><pages>799-806</pages><issn>1674-7291</issn><eissn>1869-1870</eissn><abstract>A cDNA clone related to the vernalization in winter wheat(verc203)was harvested from the en-riched cold-induced cDNA library of 10~4 pfu with differential screening.The insert of verc203 in λ gt10 vector wassubcloned into the sites between BamH Ⅰ and Hind Ⅲ in pUC19 plasmid after being amplified with PCR.the analysis of the Northern blotting with a probe of verc203 indicated that the verc203 has a negative signalfor the control and the devernalized mRNA and a positive signal for the vernalized winter wheat and non-vernalized spring wheat at about 2.6 kb.</abstract></addata></record> |
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| identifier | ISSN: 1674-7291 |
| ispartof | 中国科学：化学英文版, 1995 (7), p.799-806 |
| issn | 1674-7291 1869-1870 |
| language | eng |
| recordid | cdi_chongqing_primary_3001277630 |
| source | Alma/SFX Local Collection |
| subjects | cDNA cloning gene molecular vernalization wheat winter |
| title | Molecular cloning of a cDNA related to vernalization(verc203) in winter wheat |
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