Effects of axial ligand replacement on the redox potentialof cytochrome c

In order to demonstrate the effect of the sixth axial ligand on the biological funtionof heme proteins, we have determined the formal potentials and electron transfer numbersof imidazole (Im), 1-methylimidazole (1-MeIm) and 1-ethylimidazole (1-EtIm) complexes ofcytochrome c(cyt c) with an optically transparent thin-layer electrode.