VIMENTIN AND TUMOR DIAGNOSIS
Vimentin was isolated and purified from the pig eye lens by homogenization, ultracentrifugation, extraction in urea buffer and preparative eletrophor-esis. It was identified with SDS-PAGE and rabbit anti-vimentin was raised against the purified vimen-tin. The specificity of anti-vimentin was examine...
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| Veröffentlicht in: | 中国癌症研究：英文版 1991 (1), p.45-48 |
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| container_title | 中国癌症研究：英文版 |
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| creator | 张农 张月娥 陈忠年 张秀荣 李茂深 |
| description | Vimentin was isolated and purified from the pig eye lens by homogenization, ultracentrifugation, extraction in urea buffer and preparative eletrophor-esis. It was identified with SDS-PAGE and rabbit anti-vimentin was raised against the purified vimen-tin. The specificity of anti-vimentin was examined with immunohistochemical technique and double immune diffusion. Results showed that the vimentin antibody possessed good specificity for mesenchyme-derived cells. Tumor tissue sections from 151 cases were stained with anti-vimentin, anti-keratin, and-desmin, anti-S-100 protein, anti-Factor-FV Ⅲ related anti-gen, and anti-lysozyme. Positive staining was obtained in mesenchyme-derived cells, while the epithelial tumor cells did not react with anti-vimentin. It indicated that vimentin anti-body is effective for tumor differential diagnosis in surgical pathology. |
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It was identified with SDS-PAGE and rabbit anti-vimentin was raised against the purified vimen-tin. The specificity of anti-vimentin was examined with immunohistochemical technique and double immune diffusion. Results showed that the vimentin antibody possessed good specificity for mesenchyme-derived cells. Tumor tissue sections from 151 cases were stained with anti-vimentin, anti-keratin, and-desmin, anti-S-100 protein, anti-Factor-FV Ⅲ related anti-gen, and anti-lysozyme. Positive staining was obtained in mesenchyme-derived cells, while the epithelial tumor cells did not react with anti-vimentin. 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It was identified with SDS-PAGE and rabbit anti-vimentin was raised against the purified vimen-tin. The specificity of anti-vimentin was examined with immunohistochemical technique and double immune diffusion. Results showed that the vimentin antibody possessed good specificity for mesenchyme-derived cells. Tumor tissue sections from 151 cases were stained with anti-vimentin, anti-keratin, and-desmin, anti-S-100 protein, anti-Factor-FV Ⅲ related anti-gen, and anti-lysozyme. Positive staining was obtained in mesenchyme-derived cells, while the epithelial tumor cells did not react with anti-vimentin. It indicated that vimentin anti-body is effective for tumor differential diagnosis in surgical pathology.</description><subject>desmin</subject><subject>homogenization</subject><subject>minutes</subject><subject>pathology</subject><subject>rabbit</subject><subject>specificity</subject><subject>stained</subject><subject>staining</subject><subject>vimentin</subject><issn>1000-9604</issn><issn>1993-0631</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><recordid>eNpjYuA0tLQ01jUwMzZkAbINDAx0Lc0MTDgYuIqLswwMTM1NDQw5GWTCPH1d_UI8_RQc_VwUQkJ9_YMUXDwd3f38gz2DeRhY0xJzilN5oTQ3g7Kba4izh25yRn5eemFmXnp8QVFmbmJRZTzQfFMjSzMjYwNj4lQBAKg7KYY</recordid><startdate>1991</startdate><enddate>1991</enddate><creator>张农 张月娥 陈忠年 张秀荣 李茂深</creator><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope></search><sort><creationdate>1991</creationdate><title>VIMENTIN AND TUMOR DIAGNOSIS</title><author>张农 张月娥 陈忠年 张秀荣 李茂深</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-chongqing_primary_10052962303</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>desmin</topic><topic>homogenization</topic><topic>minutes</topic><topic>pathology</topic><topic>rabbit</topic><topic>specificity</topic><topic>stained</topic><topic>staining</topic><topic>vimentin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>张农 张月娥 陈忠年 张秀荣 李茂深</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><jtitle>中国癌症研究：英文版</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>张农 张月娥 陈忠年 张秀荣 李茂深</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>VIMENTIN AND TUMOR DIAGNOSIS</atitle><jtitle>中国癌症研究：英文版</jtitle><addtitle>Chinese Journal of Cancer Research</addtitle><date>1991</date><risdate>1991</risdate><issue>1</issue><spage>45</spage><epage>48</epage><pages>45-48</pages><issn>1000-9604</issn><eissn>1993-0631</eissn><abstract>Vimentin was isolated and purified from the pig eye lens by homogenization, ultracentrifugation, extraction in urea buffer and preparative eletrophor-esis. It was identified with SDS-PAGE and rabbit anti-vimentin was raised against the purified vimen-tin. The specificity of anti-vimentin was examined with immunohistochemical technique and double immune diffusion. Results showed that the vimentin antibody possessed good specificity for mesenchyme-derived cells. Tumor tissue sections from 151 cases were stained with anti-vimentin, anti-keratin, and-desmin, anti-S-100 protein, anti-Factor-FV Ⅲ related anti-gen, and anti-lysozyme. Positive staining was obtained in mesenchyme-derived cells, while the epithelial tumor cells did not react with anti-vimentin. It indicated that vimentin anti-body is effective for tumor differential diagnosis in surgical pathology.</abstract></addata></record> |
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| identifier | ISSN: 1000-9604 |
| ispartof | 中国癌症研究：英文版, 1991 (1), p.45-48 |
| issn | 1000-9604 1993-0631 |
| language | eng |
| recordid | cdi_chongqing_primary_1005296230 |
| source | Springer Nature - Complete Springer Journals |
| subjects | desmin homogenization minutes pathology rabbit specificity stained staining vimentin |
| title | VIMENTIN AND TUMOR DIAGNOSIS |
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