Insertional mutagenesis and cloning of HIV env 125 peptide gene by polymerase chain reaction

One oligonueleotide probe,HIV env 101-1,and two oligonucleotide primers,HIV env125-1 and HIV env 125-2,were designed with the aid of a computer and synthesized by a DNAsynthesizer.The env 125 peptide gene was amplified by polymerase chain reaetion(PCR).Beingidentified by electrophoresis and Southern...

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Veröffentlicht in:	中国人民解放军军医大学学报：英文版 1992 (3), p.222-226
1. Verfasser:	杨立宏苏成芝
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Sprache:	eng
Schlagworte:	chain cloning env gene HIV peptide polymerase reaction
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creator	杨立宏苏成芝
description	One oligonueleotide probe,HIV env 101-1,and two oligonucleotide primers,HIV env125-1 and HIV env 125-2,were designed with the aid of a computer and synthesized by a DNAsynthesizer.The env 125 peptide gene was amplified by polymerase chain reaetion(PCR).Beingidentified by electrophoresis and Southern blotting,the PCR product was cloned into plasmidpUC 19.The recombinant pENV 125,identified with X-gal selection and restriction mapping, wassequenced.The results showed that the cloned env 125 peptide gene contained the inserted EcoRⅠ site and ATG at the 5’end,Hind Ⅲ site and TAG at the 3’end.The sequence and readingframe were proved to be correct.
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subjects	chain cloning env gene HIV peptide polymerase reaction
title	Insertional mutagenesis and cloning of HIV env 125 peptide gene by polymerase chain reaction
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