Transgenic Wheat Plants Obtained With Pollen-Tube Pathway Method

Transgenic Wheat Plants Obtained With Pollen-Tube Pathway Method

β-glucuronidase (GUS) gene (carried by pBI121 plasmid) has been introduced into hexaploid wheat c. v. Xiaoshan No. 3 with the pollen-tube pathway method. Five transgenic plants are selected from 106 T0 plants. All of these plants are identified by the Southern hybridization, and the GUS activity can...

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Veröffentlicht in:中国科学：化学英文版 1994 (3), p.319-325
1. Verfasser: 曾君祉 王东江 吴有强 张健 周文娟 朱小平 徐乃正
Format: Artikel
Sprache:eng
Schlagworte:
gene
GUS
method
pathway
plants
pollen-tube
transgenic
wheat
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Zusammenfassung:β-glucuronidase (GUS) gene (carried by pBI121 plasmid) has been introduced into hexaploid wheat c. v. Xiaoshan No. 3 with the pollen-tube pathway method. Five transgenic plants are selected from 106 T0 plants. All of these plants are identified by the Southern hybridization, and the GUS activity can be detected by using fluorogenic assay and histochemical stain.
ISSN:1674-7291
1869-1870