DETECTION OF GENES FOR HEAT-STABLE ENTEROTOXIN IN ESCHERICHIA COLI BY BIOTINYLATED ST-DNA PROBES
Reference strains of enterotoxigenic Escherichia coli (ETEC), non-enterotoxigenic Escherichia coli (non-ETEC), enteropathogenic Escherichia coli (EPEC),enteroinvasive Escherichia coli (EIEC), and other enteropathogenic bacteria were used to prove the reliability of BIO-ST-DNA probe hybridization. In...
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| Veröffentlicht in: | 中华医学杂志：英文版 1994 (5), p.20-23 |
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| container_title | 中华医学杂志：英文版 |
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| creator | 朱庆义 李连青 林万明 周志江 刘纯杰 |
| description | Reference strains of enterotoxigenic Escherichia coli (ETEC), non-enterotoxigenic Escherichia coli (non-ETEC), enteropathogenic Escherichia coli (EPEC),enteroinvasive Escherichia coli (EIEC), and other enteropathogenic bacteria were used to prove the reliability of BIO-ST-DNA probe hybridization. In addition, 417 strains of E. coli isolated from children with diarrheal diseases in Shanxi Children’s Hospital were examined for BIO-ST-DNA probe hybridization. In the test, BIO-ST-DNA hybridization was compared with suckling mouse assay in identifying ST-ETEC. The results obtained by both methods showed no significant difference. It was found that identification of ST-ETEC using hybridization is a simple, sensitive and more practical method. |
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In addition, 417 strains of E. coli isolated from children with diarrheal diseases in Shanxi Children’s Hospital were examined for BIO-ST-DNA probe hybridization. In the test, BIO-ST-DNA hybridization was compared with suckling mouse assay in identifying ST-ETEC. The results obtained by both methods showed no significant difference. 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In addition, 417 strains of E. coli isolated from children with diarrheal diseases in Shanxi Children’s Hospital were examined for BIO-ST-DNA probe hybridization. In the test, BIO-ST-DNA hybridization was compared with suckling mouse assay in identifying ST-ETEC. The results obtained by both methods showed no significant difference. It was found that identification of ST-ETEC using hybridization is a simple, sensitive and more practical method.</abstract></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0366-6999 |
| ispartof | 中华医学杂志：英文版, 1994 (5), p.20-23 |
| issn | 0366-6999 2542-5641 |
| language | eng |
| recordid | cdi_chongqing_primary_1005051936 |
| source | Alma/SFX Local Collection |
| subjects | children cholera chromato DNA dUTP EPEC ETEC identifying Salmonella Shanxi |
| title | DETECTION OF GENES FOR HEAT-STABLE ENTEROTOXIN IN ESCHERICHIA COLI BY BIOTINYLATED ST-DNA PROBES |
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