An increase in opening of BKCa channels in smooth muscle cells in streptozotocin-induced diabetic mice
AIM: To investigate the changes of function of large conductance of calcium-activated potassium channels (BKCa channels) in thoracic aortic smooth muscle cells in early stage of streptozotocin (STZ)-induced diabetic C57BL/6J mice. METHODS: Vascular muscle tension in the isolated thoracic aortic ring...
Gespeichert in:
| Veröffentlicht in: | Acta pharmacologica Sinica 2004-06, Vol.25 (6), p.744-750 |
|---|---|
| 1. Verfasser: | |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 750 |
|---|---|
| container_issue | 6 |
| container_start_page | 744 |
| container_title | Acta pharmacologica Sinica |
| container_volume | 25 |
| creator | Chun-lingYE BingSHEN Xian-daREN Rong-jingLUO Sheng-yuanDING Fu-manYAN Jia-huaJIANG |
| description | AIM: To investigate the changes of function of large conductance of calcium-activated potassium channels (BKCa channels) in thoracic aortic smooth muscle cells in early stage of streptozotocin (STZ)-induced diabetic C57BL/6J mice. METHODS: Vascular muscle tension in the isolated thoracic aortic rings of mice was compared, and the role of BKCa channels in relaxation of isolated mice thoracic aortic rings induced by acetylcholine (ACh) was determined.Meanwhile, single vascular smooth muscle cells (VSMCs) were isolated by collagenase, and BKCa currents were recorded by patch-clamp single channel recording technique in symmetric high potassium solution. RESULTS:Tetraethylammonium (TEA) 1 mmol/L, a selective calcium-activated potassium channel blocker, caused significant rightward shift in the concentration-response curves of ACh in the isolated thoracic aortic rings of diabetic mice and pD2 value of ACh-induced relaxation was decreased notably after TEA treatment [(6.3±0.4) vs (6.9±0.5), n=10 rings from 7 mice, P0.05]. Furthermore,conductance of BKCa channels in single thoracic aortic smooth muscle cells was decreased [(199±15) pS, n=10 cells from 7 mice vs (266±11) pS, n=12 cells from 6 mice, P |
| format | Article |
| fullrecord | <record><control><sourceid>chongqing</sourceid><recordid>TN_cdi_chongqing_backfile_9980219</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><cqvip_id>9980219</cqvip_id><sourcerecordid>9980219</sourcerecordid><originalsourceid>FETCH-LOGICAL-c179t-8b6cf6020f35975f575bd4f676576c310904e03b5f4e741f9a736516de7e5f7e3</originalsourceid><addsrcrecordid>eNotj8tKAzEYhYMoWKvvEB8gkExuk2UdvNGCG12XzD9_OtGZpDbpxqe3Ylfn43xw4FyQhbBKM9todXliYwVTvJXX5KaUT85lI4VbkLBKNCY4oC94Apr3mGLa0Rzow7rzFEafEk7lz5U55zrS-VhgQgo4net6wH3NP7lmiInFNBwBBzpE32ONQOcIeEuugp8K3p1zST6eHt-7F7Z5e37tVhsGwrrK2t5AMLzhQWpnddBW94MKxhptDUjBHVfIZa-DQqtEcN5Ko4UZ0KIOFuWS3P_vwpjT7vv0ZNt7-Apxwq1zLW-Ek78dBFK_</addsrcrecordid><sourcetype>Publisher</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>An increase in opening of BKCa channels in smooth muscle cells in streptozotocin-induced diabetic mice</title><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><creator>Chun-lingYE BingSHEN Xian-daREN Rong-jingLUO Sheng-yuanDING Fu-manYAN Jia-huaJIANG</creator><creatorcontrib>Chun-lingYE BingSHEN Xian-daREN Rong-jingLUO Sheng-yuanDING Fu-manYAN Jia-huaJIANG</creatorcontrib><description>AIM: To investigate the changes of function of large conductance of calcium-activated potassium channels (BKCa channels) in thoracic aortic smooth muscle cells in early stage of streptozotocin (STZ)-induced diabetic C57BL/6J mice. METHODS: Vascular muscle tension in the isolated thoracic aortic rings of mice was compared, and the role of BKCa channels in relaxation of isolated mice thoracic aortic rings induced by acetylcholine (ACh) was determined.Meanwhile, single vascular smooth muscle cells (VSMCs) were isolated by collagenase, and BKCa currents were recorded by patch-clamp single channel recording technique in symmetric high potassium solution. RESULTS:Tetraethylammonium (TEA) 1 mmol/L, a selective calcium-activated potassium channel blocker, caused significant rightward shift in the concentration-response curves of ACh in the isolated thoracic aortic rings of diabetic mice and pD2 value of ACh-induced relaxation was decreased notably after TEA treatment [(6.3±0.4) vs (6.9±0.5), n=10 rings from 7 mice, P<0.01]. But pD2 value of ACh-induced relaxation in age-matched control mice did not change in presence and absence of TEA 1 mmol/L [(6.4±0.15) vs (6.5±0.5), n=7 rings from 6 mice, P>0.05]. Furthermore,conductance of BKCa channels in single thoracic aortic smooth muscle cells was decreased [(199±15) pS, n=10 cells from 7 mice vs (266±11) pS, n=12 cells from 6 mice, P<0.01], but probability of open of BKCa channels was increased [(0.51±0.28) vs (0.11±0.06), n=6 cells from 6 mice, P<0.01 ], and the mean closed time in diabetic mice was reduced [(15±15) vs (132±98), n=6 cells from 6 mice, P<0.05]. CONCLUSION: The opening of BKCa channels was increased in thoracic aortic smooth muscle ceils in the early stage of STZ-induced diabetic C57BL/6J mice by reducing mean closed time, but the conductance of BKCa channels was decreased.</description><identifier>ISSN: 1671-4083</identifier><identifier>EISSN: 1745-7254</identifier><language>eng</language><subject>ACh ; BKCa通路 ; 乙酰胆碱 ; 平滑肌细胞 ; 糖尿病 ; 诱导反应 ; 链脲霉素</subject><ispartof>Acta pharmacologica Sinica, 2004-06, Vol.25 (6), p.744-750</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/95561A/95561A.jpg</thumbnail><link.rule.ids>314,780,784</link.rule.ids></links><search><creatorcontrib>Chun-lingYE BingSHEN Xian-daREN Rong-jingLUO Sheng-yuanDING Fu-manYAN Jia-huaJIANG</creatorcontrib><title>An increase in opening of BKCa channels in smooth muscle cells in streptozotocin-induced diabetic mice</title><title>Acta pharmacologica Sinica</title><addtitle>Acta Pharmacologica Sinica</addtitle><description>AIM: To investigate the changes of function of large conductance of calcium-activated potassium channels (BKCa channels) in thoracic aortic smooth muscle cells in early stage of streptozotocin (STZ)-induced diabetic C57BL/6J mice. METHODS: Vascular muscle tension in the isolated thoracic aortic rings of mice was compared, and the role of BKCa channels in relaxation of isolated mice thoracic aortic rings induced by acetylcholine (ACh) was determined.Meanwhile, single vascular smooth muscle cells (VSMCs) were isolated by collagenase, and BKCa currents were recorded by patch-clamp single channel recording technique in symmetric high potassium solution. RESULTS:Tetraethylammonium (TEA) 1 mmol/L, a selective calcium-activated potassium channel blocker, caused significant rightward shift in the concentration-response curves of ACh in the isolated thoracic aortic rings of diabetic mice and pD2 value of ACh-induced relaxation was decreased notably after TEA treatment [(6.3±0.4) vs (6.9±0.5), n=10 rings from 7 mice, P<0.01]. But pD2 value of ACh-induced relaxation in age-matched control mice did not change in presence and absence of TEA 1 mmol/L [(6.4±0.15) vs (6.5±0.5), n=7 rings from 6 mice, P>0.05]. Furthermore,conductance of BKCa channels in single thoracic aortic smooth muscle cells was decreased [(199±15) pS, n=10 cells from 7 mice vs (266±11) pS, n=12 cells from 6 mice, P<0.01], but probability of open of BKCa channels was increased [(0.51±0.28) vs (0.11±0.06), n=6 cells from 6 mice, P<0.01 ], and the mean closed time in diabetic mice was reduced [(15±15) vs (132±98), n=6 cells from 6 mice, P<0.05]. CONCLUSION: The opening of BKCa channels was increased in thoracic aortic smooth muscle ceils in the early stage of STZ-induced diabetic C57BL/6J mice by reducing mean closed time, but the conductance of BKCa channels was decreased.</description><subject>ACh</subject><subject>BKCa通路</subject><subject>乙酰胆碱</subject><subject>平滑肌细胞</subject><subject>糖尿病</subject><subject>诱导反应</subject><subject>链脲霉素</subject><issn>1671-4083</issn><issn>1745-7254</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNotj8tKAzEYhYMoWKvvEB8gkExuk2UdvNGCG12XzD9_OtGZpDbpxqe3Ylfn43xw4FyQhbBKM9todXliYwVTvJXX5KaUT85lI4VbkLBKNCY4oC94Apr3mGLa0Rzow7rzFEafEk7lz5U55zrS-VhgQgo4net6wH3NP7lmiInFNBwBBzpE32ONQOcIeEuugp8K3p1zST6eHt-7F7Z5e37tVhsGwrrK2t5AMLzhQWpnddBW94MKxhptDUjBHVfIZa-DQqtEcN5Ko4UZ0KIOFuWS3P_vwpjT7vv0ZNt7-Apxwq1zLW-Ek78dBFK_</recordid><startdate>20040601</startdate><enddate>20040601</enddate><creator>Chun-lingYE BingSHEN Xian-daREN Rong-jingLUO Sheng-yuanDING Fu-manYAN Jia-huaJIANG</creator><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope></search><sort><creationdate>20040601</creationdate><title>An increase in opening of BKCa channels in smooth muscle cells in streptozotocin-induced diabetic mice</title><author>Chun-lingYE BingSHEN Xian-daREN Rong-jingLUO Sheng-yuanDING Fu-manYAN Jia-huaJIANG</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c179t-8b6cf6020f35975f575bd4f676576c310904e03b5f4e741f9a736516de7e5f7e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>ACh</topic><topic>BKCa通路</topic><topic>乙酰胆碱</topic><topic>平滑肌细胞</topic><topic>糖尿病</topic><topic>诱导反应</topic><topic>链脲霉素</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chun-lingYE BingSHEN Xian-daREN Rong-jingLUO Sheng-yuanDING Fu-manYAN Jia-huaJIANG</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><jtitle>Acta pharmacologica Sinica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chun-lingYE BingSHEN Xian-daREN Rong-jingLUO Sheng-yuanDING Fu-manYAN Jia-huaJIANG</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An increase in opening of BKCa channels in smooth muscle cells in streptozotocin-induced diabetic mice</atitle><jtitle>Acta pharmacologica Sinica</jtitle><addtitle>Acta Pharmacologica Sinica</addtitle><date>2004-06-01</date><risdate>2004</risdate><volume>25</volume><issue>6</issue><spage>744</spage><epage>750</epage><pages>744-750</pages><issn>1671-4083</issn><eissn>1745-7254</eissn><abstract>AIM: To investigate the changes of function of large conductance of calcium-activated potassium channels (BKCa channels) in thoracic aortic smooth muscle cells in early stage of streptozotocin (STZ)-induced diabetic C57BL/6J mice. METHODS: Vascular muscle tension in the isolated thoracic aortic rings of mice was compared, and the role of BKCa channels in relaxation of isolated mice thoracic aortic rings induced by acetylcholine (ACh) was determined.Meanwhile, single vascular smooth muscle cells (VSMCs) were isolated by collagenase, and BKCa currents were recorded by patch-clamp single channel recording technique in symmetric high potassium solution. RESULTS:Tetraethylammonium (TEA) 1 mmol/L, a selective calcium-activated potassium channel blocker, caused significant rightward shift in the concentration-response curves of ACh in the isolated thoracic aortic rings of diabetic mice and pD2 value of ACh-induced relaxation was decreased notably after TEA treatment [(6.3±0.4) vs (6.9±0.5), n=10 rings from 7 mice, P<0.01]. But pD2 value of ACh-induced relaxation in age-matched control mice did not change in presence and absence of TEA 1 mmol/L [(6.4±0.15) vs (6.5±0.5), n=7 rings from 6 mice, P>0.05]. Furthermore,conductance of BKCa channels in single thoracic aortic smooth muscle cells was decreased [(199±15) pS, n=10 cells from 7 mice vs (266±11) pS, n=12 cells from 6 mice, P<0.01], but probability of open of BKCa channels was increased [(0.51±0.28) vs (0.11±0.06), n=6 cells from 6 mice, P<0.01 ], and the mean closed time in diabetic mice was reduced [(15±15) vs (132±98), n=6 cells from 6 mice, P<0.05]. CONCLUSION: The opening of BKCa channels was increased in thoracic aortic smooth muscle ceils in the early stage of STZ-induced diabetic C57BL/6J mice by reducing mean closed time, but the conductance of BKCa channels was decreased.</abstract><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1671-4083 |
| ispartof | Acta pharmacologica Sinica, 2004-06, Vol.25 (6), p.744-750 |
| issn | 1671-4083 1745-7254 |
| language | eng |
| recordid | cdi_chongqing_backfile_9980219 |
| source | Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | ACh BKCa通路 乙酰胆碱 平滑肌细胞 糖尿病 诱导反应 链脲霉素 |
| title | An increase in opening of BKCa channels in smooth muscle cells in streptozotocin-induced diabetic mice |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-11T08%3A09%3A16IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-chongqing&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=An%20increase%20in%20opening%20of%20BKCa%20channels%20in%20smooth%20muscle%20cells%20in%20streptozotocin-induced%20diabetic%20mice&rft.jtitle=Acta%20pharmacologica%20Sinica&rft.au=Chun-lingYE%20BingSHEN%20Xian-daREN%20Rong-jingLUO%20Sheng-yuanDING%20Fu-manYAN%20Jia-huaJIANG&rft.date=2004-06-01&rft.volume=25&rft.issue=6&rft.spage=744&rft.epage=750&rft.pages=744-750&rft.issn=1671-4083&rft.eissn=1745-7254&rft_id=info:doi/&rft_dat=%3Cchongqing%3E9980219%3C/chongqing%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rft_cqvip_id=9980219&rfr_iscdi=true |