NQ01 C609T polymorphism associated with esophageal cancer and gastric cardiac carcinoma in North China

AIM: To investigate the association of the/VQO1 (C609T) polymorphism with susceptibility to esophageal squamous cell carcinoma (ESCC) and gastric cardiac adenocarcinoma (GCA) in North China. METHODS: The NQ01 C609T genotypes were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis in 317 cancer patients (193 ESCC and 124 GCA) and 165 unrelated healthy controls. RESULTS: The NQ01 C609T C/C, C/T and T/T genotype frequency among healthy controls was 31.5 %, 52.1% and 16.4 % respectively. The NQ01 T/T genotype frequency among ESCC patients (25.9 %) was significantly higher than that among healthy controls (X~2=4.79, P=0.028). The NQ01 T/T genotype significantly increased the risk for developing ESCC compared with the combination of C/C and C/T genotypes, with an age, sex and smoking status adjusted odds ratio (OR) of 1.78 (1.04-2.98). This increased susceptibility was pronounced in ESCC patients with family histories of upper gastrointestinal cancers (UGIC) (adjusted OR=2.20, 95 % CI=1.18-3.98). Similarly, the susceptibility of the NQ01 T/T genotype to GCA development was also observed among patients with family histories of UGIC, with an adjusted odds ratio of 2.55 (95 % CI=1.21-5.23), whereas no differencein NQ01 genotype distribution was shown among patients without family histories of UGIC .CONCLUSION: Determination of the IVQOIC609T genotype may be used as a stratification marker to predicate the individuals at high risk for developing ESCC and GCA in North China.