西花蓟马(Frankliniella occidentalis)rDNA ITS2和COⅠ基因5’末端序列的克隆与比较分析
利用PCR技术克隆西花蓟马(Frankliniella occidentalis)的rDNAITS2和COⅠ基因5’末端的部分序列,结合GenBank中相应序列对两者的突变类型、核苷酸多态性、单倍型多态性、种内平均遗传距离以及系统进化等进行比较分析.结果表明,COⅠ基因较rDNAITs2更适合于F.occidentalis的种内遗传分析;F.occidentalis可能是由两个(或多个)株系或隐存种组成的复合体;入侵我国的F.occidentalis以温室系(greenhouse strain)为主体,也存在一定数量的羽扇豆系(lupinstrain).据此,推测F.occidentalis最...
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creator | 武晓云 程晓非 张仲凯 桂富荣 李正跃 |
description | 利用PCR技术克隆西花蓟马(Frankliniella occidentalis)的rDNAITS2和COⅠ基因5’末端的部分序列,结合GenBank中相应序列对两者的突变类型、核苷酸多态性、单倍型多态性、种内平均遗传距离以及系统进化等进行比较分析.结果表明,COⅠ基因较rDNAITs2更适合于F.occidentalis的种内遗传分析;F.occidentalis可能是由两个(或多个)株系或隐存种组成的复合体;入侵我国的F.occidentalis以温室系(greenhouse strain)为主体,也存在一定数量的羽扇豆系(lupinstrain).据此,推测F.occidentalis最先是被传播至云南,再从云南扩散至北京、哈尔滨等地.本研究结果对了解我国F.occidentalis的遗传多样性、株系组成以及入侵机制具有一定意义. |
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source | EZB-FREE-00999 freely available EZB journals |
subjects | 入侵机制 株系组成 西花蓟马 遗传分析 |
title | 西花蓟马(Frankliniella occidentalis)rDNA ITS2和COⅠ基因5’末端序列的克隆与比较分析 |
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