Antitumour effects on human colorectal carcinomas cells by stable silencing of phospholipase C-gamma I with lentivirus-delivered siRNA
Background In most colorectal carcinomas, the level of phospholipase C (PLC)-gamma 1 expression is greatly elevated. Increased expression of PLC-gamma 1 may play an important role in colon carcinogenesis, but the mechanism is not well known. The aim of this study was to evaluate the role of PLC-gamm...
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Veröffentlicht in: | Chinese medical journal 2007, Vol.120 (9), p.749-754 |
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creator | TAN Li XIAO Bing-xiang ZENG Wei-sen LIN Jun ZOU Zhi-peng XU Ai-min LUO Shen-qiu |
description | Background In most colorectal carcinomas, the level of phospholipase C (PLC)-gamma 1 expression is greatly elevated. Increased expression of PLC-gamma 1 may play an important role in colon carcinogenesis, but the mechanism is not well known. The aim of this study was to evaluate the role of PLC-gamma 1 in colon carcinogenesis by using recombinant lentivirus that stably suppressed the PLC-gamma 1 expression in human colorectal carcinoma LoVo cells.
Methods Recombinant lentivirus producing PLC-gamma 1 siRNA were prepared. After LoVo cells were transduced by each lentivirus, stably transduced cells were selected by Blasticidin. The protein and mRNA expression of PLC-gamma 1 were examined by Western-blot and reverse transcription-polymerase chain reaction (RT-PCR) analysis, and the effects of the lentivirus on the cell adhesion, migration and apoptosis were analyzed.
Results Stable LoVo cell line deficient in PLC-gamma 1, was established. Notably, PLC-gamma 1 was silenced without affecting the levels of other subtypes of PLC so that the role of PLC-gamma 1 in colon carcinogenesis could be examined Silencing of endogenous PLC-gamma 1 resulted in efficient inhibition of the adhesion and migration of LoVo cells in vitro and a great increase of 5-fluorouracil induced apoptosis (30%-40%) of LoVo cells.
Conclusions PLC-gamma 1 may play an important role in metastasis and anti-apoptosis in human colorectal carcinomas. |
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Methods Recombinant lentivirus producing PLC-gamma 1 siRNA were prepared. After LoVo cells were transduced by each lentivirus, stably transduced cells were selected by Blasticidin. The protein and mRNA expression of PLC-gamma 1 were examined by Western-blot and reverse transcription-polymerase chain reaction (RT-PCR) analysis, and the effects of the lentivirus on the cell adhesion, migration and apoptosis were analyzed.
Results Stable LoVo cell line deficient in PLC-gamma 1, was established. Notably, PLC-gamma 1 was silenced without affecting the levels of other subtypes of PLC so that the role of PLC-gamma 1 in colon carcinogenesis could be examined Silencing of endogenous PLC-gamma 1 resulted in efficient inhibition of the adhesion and migration of LoVo cells in vitro and a great increase of 5-fluorouracil induced apoptosis (30%-40%) of LoVo cells.
Conclusions PLC-gamma 1 may play an important role in metastasis and anti-apoptosis in human colorectal carcinomas.</description><identifier>ISSN: 0366-6999</identifier><identifier>EISSN: 2542-5641</identifier><language>eng</language><subject>siRNA ; 基因稳定沉默 ; 慢病毒载体 ; 磷脂酶C-γ1 ; 结直肠癌细胞</subject><ispartof>Chinese medical journal, 2007, Vol.120 (9), p.749-754</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://image.cqvip.com/vip1000/qk/85656X/85656X.jpg</thumbnail><link.rule.ids>314,780,784,4024</link.rule.ids></links><search><creatorcontrib>TAN Li XIAO Bing-xiang ZENG Wei-sen LIN Jun ZOU Zhi-peng XU Ai-min LUO Shen-qiu</creatorcontrib><title>Antitumour effects on human colorectal carcinomas cells by stable silencing of phospholipase C-gamma I with lentivirus-delivered siRNA</title><title>Chinese medical journal</title><addtitle>Chinese Medical Journal</addtitle><description>Background In most colorectal carcinomas, the level of phospholipase C (PLC)-gamma 1 expression is greatly elevated. Increased expression of PLC-gamma 1 may play an important role in colon carcinogenesis, but the mechanism is not well known. The aim of this study was to evaluate the role of PLC-gamma 1 in colon carcinogenesis by using recombinant lentivirus that stably suppressed the PLC-gamma 1 expression in human colorectal carcinoma LoVo cells.
Methods Recombinant lentivirus producing PLC-gamma 1 siRNA were prepared. After LoVo cells were transduced by each lentivirus, stably transduced cells were selected by Blasticidin. The protein and mRNA expression of PLC-gamma 1 were examined by Western-blot and reverse transcription-polymerase chain reaction (RT-PCR) analysis, and the effects of the lentivirus on the cell adhesion, migration and apoptosis were analyzed.
Results Stable LoVo cell line deficient in PLC-gamma 1, was established. Notably, PLC-gamma 1 was silenced without affecting the levels of other subtypes of PLC so that the role of PLC-gamma 1 in colon carcinogenesis could be examined Silencing of endogenous PLC-gamma 1 resulted in efficient inhibition of the adhesion and migration of LoVo cells in vitro and a great increase of 5-fluorouracil induced apoptosis (30%-40%) of LoVo cells.
Conclusions PLC-gamma 1 may play an important role in metastasis and anti-apoptosis in human colorectal carcinomas.</description><subject>siRNA</subject><subject>基因稳定沉默</subject><subject>慢病毒载体</subject><subject>磷脂酶C-γ1</subject><subject>结直肠癌细胞</subject><issn>0366-6999</issn><issn>2542-5641</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNqNTUtOwzAQtRBIhM8dRuwjpaltyLKqQLBhgbqvJu44MfhTPE4RF-DceMEBWDw96X3PRNMr2bdKy9W5aLq11q0ehuFSXDG_d12v1L1uxM8mFleWkJYMZC2ZwpAizEvACCb5lKuEHgxm42IKyGDIe4bxG7jg6AnYeYrVnCBZOM6JK7w7IhNs2wlDQHiBL1dmqLniTi4v3B7IuxNlOtT62-vmRlxY9Ey3f3wt7p4ed9vn1swpTp91fD-i-bD1at9LqR7USq7_FfoFYHxUFw</recordid><startdate>2007</startdate><enddate>2007</enddate><creator>TAN Li XIAO Bing-xiang ZENG Wei-sen LIN Jun ZOU Zhi-peng XU Ai-min LUO Shen-qiu</creator><scope>2RA</scope><scope>92L</scope><scope>CQIGP</scope><scope>W91</scope><scope>~WA</scope></search><sort><creationdate>2007</creationdate><title>Antitumour effects on human colorectal carcinomas cells by stable silencing of phospholipase C-gamma I with lentivirus-delivered siRNA</title><author>TAN Li XIAO Bing-xiang ZENG Wei-sen LIN Jun ZOU Zhi-peng XU Ai-min LUO Shen-qiu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-chongqing_backfile_244585143</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>siRNA</topic><topic>基因稳定沉默</topic><topic>慢病毒载体</topic><topic>磷脂酶C-γ1</topic><topic>结直肠癌细胞</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>TAN Li XIAO Bing-xiang ZENG Wei-sen LIN Jun ZOU Zhi-peng XU Ai-min LUO Shen-qiu</creatorcontrib><collection>中文科技期刊数据库</collection><collection>中文科技期刊数据库-CALIS站点</collection><collection>中文科技期刊数据库-7.0平台</collection><collection>中文科技期刊数据库-医药卫生</collection><collection>中文科技期刊数据库- 镜像站点</collection><jtitle>Chinese medical journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>TAN Li XIAO Bing-xiang ZENG Wei-sen LIN Jun ZOU Zhi-peng XU Ai-min LUO Shen-qiu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antitumour effects on human colorectal carcinomas cells by stable silencing of phospholipase C-gamma I with lentivirus-delivered siRNA</atitle><jtitle>Chinese medical journal</jtitle><addtitle>Chinese Medical Journal</addtitle><date>2007</date><risdate>2007</risdate><volume>120</volume><issue>9</issue><spage>749</spage><epage>754</epage><pages>749-754</pages><issn>0366-6999</issn><eissn>2542-5641</eissn><abstract>Background In most colorectal carcinomas, the level of phospholipase C (PLC)-gamma 1 expression is greatly elevated. Increased expression of PLC-gamma 1 may play an important role in colon carcinogenesis, but the mechanism is not well known. The aim of this study was to evaluate the role of PLC-gamma 1 in colon carcinogenesis by using recombinant lentivirus that stably suppressed the PLC-gamma 1 expression in human colorectal carcinoma LoVo cells.
Methods Recombinant lentivirus producing PLC-gamma 1 siRNA were prepared. After LoVo cells were transduced by each lentivirus, stably transduced cells were selected by Blasticidin. The protein and mRNA expression of PLC-gamma 1 were examined by Western-blot and reverse transcription-polymerase chain reaction (RT-PCR) analysis, and the effects of the lentivirus on the cell adhesion, migration and apoptosis were analyzed.
Results Stable LoVo cell line deficient in PLC-gamma 1, was established. Notably, PLC-gamma 1 was silenced without affecting the levels of other subtypes of PLC so that the role of PLC-gamma 1 in colon carcinogenesis could be examined Silencing of endogenous PLC-gamma 1 resulted in efficient inhibition of the adhesion and migration of LoVo cells in vitro and a great increase of 5-fluorouracil induced apoptosis (30%-40%) of LoVo cells.
Conclusions PLC-gamma 1 may play an important role in metastasis and anti-apoptosis in human colorectal carcinomas.</abstract></addata></record> |
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source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
subjects | siRNA 基因稳定沉默 慢病毒载体 磷脂酶C-γ1 结直肠癌细胞 |
title | Antitumour effects on human colorectal carcinomas cells by stable silencing of phospholipase C-gamma I with lentivirus-delivered siRNA |
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