Comparison between two McMaster egg counting slide used for the diagnostic of gastrointestinal nematode infection in ruminants
Gastrointestinal parasitism in ruminants, represents a major constraint to growth, reproduction and survival in susceptible animals. The control of parasitic infections need of techniques able to determine the presence of the parasite and the infestation levels within the herd in order to establish...
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Veröffentlicht in: | Zootecnia tropical 2013-04, Vol.29 (4) |
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Zusammenfassung: | Gastrointestinal parasitism in ruminants, represents a major constraint
to growth, reproduction and survival in susceptible animals. The
control of parasitic infections need of techniques able to determine
the presence of the parasite and the infestation levels within the herd
in order to establish the possible relationship among the physiologic
alterations, the level of parasitic infection and the affection on the
production. The aims of this work, was to compare the classical
coproscopical technique of McMaster (TCC) against the McMaster INTA
(TCI) technique, both are eggs counting slide used for the
parasitological diagnosis. For the study 40 crossbreed calves of both
sexes were used. A total of 40 samples of faeces were gathered directly
of the rectum of each animal and examined for both techniques. The
comparison between the eggs per gram of faeces counts (HPG) was
realized by mean of Wilcoxon test and the correlation through the
coeffi cient of ranks of Spearman. The comparison of the discrepancies
was made with the McNemar test and the agreement was evaluated with the
Kappa Index. We conclude that both cameras have features of
reliability, speed, simplicity and low cost, but the TCI offers
advantages in detection of eggs (positive animals) and accumulators
animal identification.
El parasitismo gastrointestinal en rumiantes, representa una importante
limitante del crecimiento, reproducción y supervivencia en
animales susceptibles. Para su control debe disponerse de técnicas
diagnósticas capaces de determinar la presencia del parásito,
los niveles de infección y permitir inferir en base a estos
resultados, elestado de alteraciones fi siológicas y el grado de
afección sobre factores de importancia en la producción que
estas generan. El objetivo de este trabajo consistió en comparar
la efi cacia de dos cámaras diseñadas para el conteo
coproscópico de huevos de estróngilos digestivos. Para el
estudio se emplearon 40 becerros mestizos de ambos sexos, de los cuales
se recolectaron 40 muestras de heces directamente del recto de cada
animal para su posterior procesamiento por las técnicas de
McMaster clásica modificada (TCC) que consta de dos
compartimientos y requiere de una muestra de 3 g de heces, y la
técnica de McMaster modifi cada empleando la cámara INTA
(TCI), que posee 4 compartimientos y requiere de una muestra de 5 g de
heces. Para la comparación entre los recuentos de huevos de
estróngilos digestivos por gramo de heces (HPG) se empleó la
prueba d |
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ISSN: | 0798-7269 |