Effect of polyunsaturated fatty acids on secretory phospholipase A2 type IIa in ectopic endometrial cells
Background: Endometriosis is a common chronic inflammation which leads to infertility and chronic pelvic pain in affected women. Secretory phospholipase A2 type IIa (sPLA2IIa) is an acute phase reactant that is markedly increased in inflammatory disorders. Objective: To assess the effects of ω-3 and...
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| creator | Khanaki, Korosh Ardekani, Ali Motavalizadeh Ghassemzadeh, Alieh Shahnazi, Vahideh Sadeghi, Mohammad Reza Darabi, Masoud Mehdizadeh, Amir Saremi, Abotaleb Soleimani-Rad, Jafar Imani, Ali Reza Nouri, Mohammad Rahimipour, Ali |
| description | Background: Endometriosis is a common chronic inflammation which leads
to infertility and chronic pelvic pain in affected women. Secretory
phospholipase A2 type IIa (sPLA2IIa) is an acute phase reactant that is
markedly increased in inflammatory disorders. Objective: To assess the
effects of ω-3 and ω-6 polyunsaturated fatty acids (PUFAs)
administration in endometrial cells culture on sPLA2IIa level and cell
survival comparing homolog ectopic versus eutopic endometrial cells
from endometriosis patients. Materials and Methods: In this
experimental study, ectopic and eutopic endometrial tissue samples
obtained from 15 endometriosis patients were immediately frozen. After
thawing and tissue digestion, mixed stromal and endometrial gland cells
were cultured for 8 days in three different culture media; balanced
ω-3/ω-6, high ω-3 and high ω-6 PUFAs ratio. Cell
survival was measured using 2, 3-bis
(2-methoxy-4-nitro-5-sulfophenyl)-5-(phenylamino) carbonyl-2H-
tetrazolium hydroxide (XTT) method and sPLA2IIa level assessed with
ELISA technique. Results: The sPLA2IIa level was significantly higher
in the ectopic endometrial cell culture compared to the eutopic group
for each of the three matched treatments (balanced, high ω-3 and
high ω-6). Also the sPLA2IIa level in the ectopic endometrial cell
group was remarkably increased by each of the three PUFAs treatments
compared to control condition (p |
| format | Article |
| fullrecord | <record><control><sourceid>bioline</sourceid><recordid>TN_cdi_bioline_primary_cria_bioline_rm_rm12046</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>cria_bioline_rm_rm12046</sourcerecordid><originalsourceid>FETCH-bioline_primary_cria_bioline_rm_rm120463</originalsourceid><addsrcrecordid>eNqVjEsKAjEQRLNQ8HuHvoAyP4JbEcXZux_aTAcjmSSkM4vc3iz0AEIVBQXvLcS6lqfqILu2XYkN87uqZC2bdi3MVWtSCbyG4G2eHWOaIyYaQWNKGVCZkcE7YFKRko8ZwstzqTUBmeDcQMqBoO8RjIMi88EoIDf6iVI0aEGRtbwTS42Waf_drTjero_L_fA0ReVoCNFMGPOgCjP8zjiV1E3VyfZv4AN_qVKU</addsrcrecordid><sourcetype>Publisher</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Effect of polyunsaturated fatty acids on secretory phospholipase A2 type IIa in ectopic endometrial cells</title><source>DOAJ Directory of Open Access Journals</source><source>PubMed Central</source><source>PubMed Central Open Access</source><creator>Khanaki, Korosh ; Ardekani, Ali Motavalizadeh ; Ghassemzadeh, Alieh ; Shahnazi, Vahideh ; Sadeghi, Mohammad Reza ; Darabi, Masoud ; Mehdizadeh, Amir ; Saremi, Abotaleb ; Soleimani-Rad, Jafar ; Imani, Ali Reza ; Nouri, Mohammad ; Rahimipour, Ali</creator><creatorcontrib>Khanaki, Korosh ; Ardekani, Ali Motavalizadeh ; Ghassemzadeh, Alieh ; Shahnazi, Vahideh ; Sadeghi, Mohammad Reza ; Darabi, Masoud ; Mehdizadeh, Amir ; Saremi, Abotaleb ; Soleimani-Rad, Jafar ; Imani, Ali Reza ; Nouri, Mohammad ; Rahimipour, Ali</creatorcontrib><description>Background: Endometriosis is a common chronic inflammation which leads
to infertility and chronic pelvic pain in affected women. Secretory
phospholipase A2 type IIa (sPLA2IIa) is an acute phase reactant that is
markedly increased in inflammatory disorders. Objective: To assess the
effects of ω-3 and ω-6 polyunsaturated fatty acids (PUFAs)
administration in endometrial cells culture on sPLA2IIa level and cell
survival comparing homolog ectopic versus eutopic endometrial cells
from endometriosis patients. Materials and Methods: In this
experimental study, ectopic and eutopic endometrial tissue samples
obtained from 15 endometriosis patients were immediately frozen. After
thawing and tissue digestion, mixed stromal and endometrial gland cells
were cultured for 8 days in three different culture media; balanced
ω-3/ω-6, high ω-3 and high ω-6 PUFAs ratio. Cell
survival was measured using 2, 3-bis
(2-methoxy-4-nitro-5-sulfophenyl)-5-(phenylamino) carbonyl-2H-
tetrazolium hydroxide (XTT) method and sPLA2IIa level assessed with
ELISA technique. Results: The sPLA2IIa level was significantly higher
in the ectopic endometrial cell culture compared to the eutopic group
for each of the three matched treatments (balanced, high ω-3 and
high ω-6). Also the sPLA2IIa level in the ectopic endometrial cell
group was remarkably increased by each of the three PUFAs treatments
compared to control condition (p <0.05, p <0.01, p <0.05
respectively). Cell survival in the eutopic group was significantly
decreased by high ω-6 culturing compared to control medium (p
<0.05). Conclusion: The increase in sPLA2IIa level in ectopic
endometrial cells by fatty acid treatments (especially high ω-3),
strengthens the hypothesis that PUFAs stimulate secretion of cytokines
leading to increased sPLA2IIa level.</description><identifier>ISSN: 1680-6433</identifier><language>eng</language><publisher>Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd</publisher><subject>Fatty acids, Endometriosis, Secretory phospholipase A2 type IIa, Cell culture</subject><ispartof>Iranian journal of reproductive medicine, 2013-02, Vol.10 (4)</ispartof><rights>Copyright 2012 - Iranian Journal of Reproductive Medicine</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids></links><search><creatorcontrib>Khanaki, Korosh</creatorcontrib><creatorcontrib>Ardekani, Ali Motavalizadeh</creatorcontrib><creatorcontrib>Ghassemzadeh, Alieh</creatorcontrib><creatorcontrib>Shahnazi, Vahideh</creatorcontrib><creatorcontrib>Sadeghi, Mohammad Reza</creatorcontrib><creatorcontrib>Darabi, Masoud</creatorcontrib><creatorcontrib>Mehdizadeh, Amir</creatorcontrib><creatorcontrib>Saremi, Abotaleb</creatorcontrib><creatorcontrib>Soleimani-Rad, Jafar</creatorcontrib><creatorcontrib>Imani, Ali Reza</creatorcontrib><creatorcontrib>Nouri, Mohammad</creatorcontrib><creatorcontrib>Rahimipour, Ali</creatorcontrib><title>Effect of polyunsaturated fatty acids on secretory phospholipase A2 type IIa in ectopic endometrial cells</title><title>Iranian journal of reproductive medicine</title><description>Background: Endometriosis is a common chronic inflammation which leads
to infertility and chronic pelvic pain in affected women. Secretory
phospholipase A2 type IIa (sPLA2IIa) is an acute phase reactant that is
markedly increased in inflammatory disorders. Objective: To assess the
effects of ω-3 and ω-6 polyunsaturated fatty acids (PUFAs)
administration in endometrial cells culture on sPLA2IIa level and cell
survival comparing homolog ectopic versus eutopic endometrial cells
from endometriosis patients. Materials and Methods: In this
experimental study, ectopic and eutopic endometrial tissue samples
obtained from 15 endometriosis patients were immediately frozen. After
thawing and tissue digestion, mixed stromal and endometrial gland cells
were cultured for 8 days in three different culture media; balanced
ω-3/ω-6, high ω-3 and high ω-6 PUFAs ratio. Cell
survival was measured using 2, 3-bis
(2-methoxy-4-nitro-5-sulfophenyl)-5-(phenylamino) carbonyl-2H-
tetrazolium hydroxide (XTT) method and sPLA2IIa level assessed with
ELISA technique. Results: The sPLA2IIa level was significantly higher
in the ectopic endometrial cell culture compared to the eutopic group
for each of the three matched treatments (balanced, high ω-3 and
high ω-6). Also the sPLA2IIa level in the ectopic endometrial cell
group was remarkably increased by each of the three PUFAs treatments
compared to control condition (p <0.05, p <0.01, p <0.05
respectively). Cell survival in the eutopic group was significantly
decreased by high ω-6 culturing compared to control medium (p
<0.05). Conclusion: The increase in sPLA2IIa level in ectopic
endometrial cells by fatty acid treatments (especially high ω-3),
strengthens the hypothesis that PUFAs stimulate secretion of cytokines
leading to increased sPLA2IIa level.</description><subject>Fatty acids, Endometriosis, Secretory phospholipase A2 type IIa, Cell culture</subject><issn>1680-6433</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>RBI</sourceid><recordid>eNqVjEsKAjEQRLNQ8HuHvoAyP4JbEcXZux_aTAcjmSSkM4vc3iz0AEIVBQXvLcS6lqfqILu2XYkN87uqZC2bdi3MVWtSCbyG4G2eHWOaIyYaQWNKGVCZkcE7YFKRko8ZwstzqTUBmeDcQMqBoO8RjIMi88EoIDf6iVI0aEGRtbwTS42Waf_drTjero_L_fA0ReVoCNFMGPOgCjP8zjiV1E3VyfZv4AN_qVKU</recordid><startdate>20130205</startdate><enddate>20130205</enddate><creator>Khanaki, Korosh</creator><creator>Ardekani, Ali Motavalizadeh</creator><creator>Ghassemzadeh, Alieh</creator><creator>Shahnazi, Vahideh</creator><creator>Sadeghi, Mohammad Reza</creator><creator>Darabi, Masoud</creator><creator>Mehdizadeh, Amir</creator><creator>Saremi, Abotaleb</creator><creator>Soleimani-Rad, Jafar</creator><creator>Imani, Ali Reza</creator><creator>Nouri, Mohammad</creator><creator>Rahimipour, Ali</creator><general>Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd</general><scope>RBI</scope></search><sort><creationdate>20130205</creationdate><title>Effect of polyunsaturated fatty acids on secretory phospholipase A2 type IIa in ectopic endometrial cells</title><author>Khanaki, Korosh ; Ardekani, Ali Motavalizadeh ; Ghassemzadeh, Alieh ; Shahnazi, Vahideh ; Sadeghi, Mohammad Reza ; Darabi, Masoud ; Mehdizadeh, Amir ; Saremi, Abotaleb ; Soleimani-Rad, Jafar ; Imani, Ali Reza ; Nouri, Mohammad ; Rahimipour, Ali</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-bioline_primary_cria_bioline_rm_rm120463</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Fatty acids, Endometriosis, Secretory phospholipase A2 type IIa, Cell culture</topic><toplevel>online_resources</toplevel><creatorcontrib>Khanaki, Korosh</creatorcontrib><creatorcontrib>Ardekani, Ali Motavalizadeh</creatorcontrib><creatorcontrib>Ghassemzadeh, Alieh</creatorcontrib><creatorcontrib>Shahnazi, Vahideh</creatorcontrib><creatorcontrib>Sadeghi, Mohammad Reza</creatorcontrib><creatorcontrib>Darabi, Masoud</creatorcontrib><creatorcontrib>Mehdizadeh, Amir</creatorcontrib><creatorcontrib>Saremi, Abotaleb</creatorcontrib><creatorcontrib>Soleimani-Rad, Jafar</creatorcontrib><creatorcontrib>Imani, Ali Reza</creatorcontrib><creatorcontrib>Nouri, Mohammad</creatorcontrib><creatorcontrib>Rahimipour, Ali</creatorcontrib><collection>Bioline International</collection><jtitle>Iranian journal of reproductive medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Khanaki, Korosh</au><au>Ardekani, Ali Motavalizadeh</au><au>Ghassemzadeh, Alieh</au><au>Shahnazi, Vahideh</au><au>Sadeghi, Mohammad Reza</au><au>Darabi, Masoud</au><au>Mehdizadeh, Amir</au><au>Saremi, Abotaleb</au><au>Soleimani-Rad, Jafar</au><au>Imani, Ali Reza</au><au>Nouri, Mohammad</au><au>Rahimipour, Ali</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of polyunsaturated fatty acids on secretory phospholipase A2 type IIa in ectopic endometrial cells</atitle><jtitle>Iranian journal of reproductive medicine</jtitle><date>2013-02-05</date><risdate>2013</risdate><volume>10</volume><issue>4</issue><issn>1680-6433</issn><abstract>Background: Endometriosis is a common chronic inflammation which leads
to infertility and chronic pelvic pain in affected women. Secretory
phospholipase A2 type IIa (sPLA2IIa) is an acute phase reactant that is
markedly increased in inflammatory disorders. Objective: To assess the
effects of ω-3 and ω-6 polyunsaturated fatty acids (PUFAs)
administration in endometrial cells culture on sPLA2IIa level and cell
survival comparing homolog ectopic versus eutopic endometrial cells
from endometriosis patients. Materials and Methods: In this
experimental study, ectopic and eutopic endometrial tissue samples
obtained from 15 endometriosis patients were immediately frozen. After
thawing and tissue digestion, mixed stromal and endometrial gland cells
were cultured for 8 days in three different culture media; balanced
ω-3/ω-6, high ω-3 and high ω-6 PUFAs ratio. Cell
survival was measured using 2, 3-bis
(2-methoxy-4-nitro-5-sulfophenyl)-5-(phenylamino) carbonyl-2H-
tetrazolium hydroxide (XTT) method and sPLA2IIa level assessed with
ELISA technique. Results: The sPLA2IIa level was significantly higher
in the ectopic endometrial cell culture compared to the eutopic group
for each of the three matched treatments (balanced, high ω-3 and
high ω-6). Also the sPLA2IIa level in the ectopic endometrial cell
group was remarkably increased by each of the three PUFAs treatments
compared to control condition (p <0.05, p <0.01, p <0.05
respectively). Cell survival in the eutopic group was significantly
decreased by high ω-6 culturing compared to control medium (p
<0.05). Conclusion: The increase in sPLA2IIa level in ectopic
endometrial cells by fatty acid treatments (especially high ω-3),
strengthens the hypothesis that PUFAs stimulate secretion of cytokines
leading to increased sPLA2IIa level.</abstract><pub>Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences of Yazd</pub></addata></record> |
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| identifier | ISSN: 1680-6433 |
| ispartof | Iranian journal of reproductive medicine, 2013-02, Vol.10 (4) |
| issn | 1680-6433 |
| language | eng |
| recordid | cdi_bioline_primary_cria_bioline_rm_rm12046 |
| source | DOAJ Directory of Open Access Journals; PubMed Central; PubMed Central Open Access |
| subjects | Fatty acids, Endometriosis, Secretory phospholipase A2 type IIa, Cell culture |
| title | Effect of polyunsaturated fatty acids on secretory phospholipase A2 type IIa in ectopic endometrial cells |
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