Short Communication - Evaluation of a modified culture medium for Borrelia burgdorferi sensu lato
The aim of the present study was to assess the possible use of a modified medium, prepared in the laboratory using the constituents of Barbour-Stonner-Kelly (BSK) medium and medium 199 as base, for the culture of Borrelia strains, comparing the growth of individual strains in this medium and in the...
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| Veröffentlicht in: | Memórias do Instituto Oswaldo Cruz 2008-03, Vol.102 (8) |
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| container_title | Memórias do Instituto Oswaldo Cruz |
| container_volume | 102 |
| creator | Rodríguez, Islay Lienhard, Reto Gern, Lise Veuve, Marie Colette Jouda, Fatima Siegrist, Hans H Fernández, Carmen Rodríguez, José Enrique |
| description | The aim of the present study was to assess the possible use of a
modified medium, prepared in the laboratory using the constituents of
Barbour-Stonner-Kelly (BSK) medium and medium 199 as base, for the
culture of Borrelia strains, comparing the growth of individual strains
in this medium and in the BSK-H medium, and the protein profile and
antigenic characteristics of Borrelia proteins expressed in these
media. A qualitative evaluation of growth of Borrelia species was made
with acceptable results (morphology and motility), but during a
quantitative evaluation using the three main genospecies of Borrelia,
the better results were obtained with a B. burgdorferi sensu stricto
strain. The modified medium did not enable the growth of a B. afzelii
strain. The protein profile and antigenic characteristic of the
expressed proteins in the modified medium were studied with
satisfactory results. These results suggest the modified medium as an
alternative for the cultivation of Borrelia strains, with some
limitations, in poorly-resourced laboratories. |
| format | Article |
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modified medium, prepared in the laboratory using the constituents of
Barbour-Stonner-Kelly (BSK) medium and medium 199 as base, for the
culture of Borrelia strains, comparing the growth of individual strains
in this medium and in the BSK-H medium, and the protein profile and
antigenic characteristics of Borrelia proteins expressed in these
media. A qualitative evaluation of growth of Borrelia species was made
with acceptable results (morphology and motility), but during a
quantitative evaluation using the three main genospecies of Borrelia,
the better results were obtained with a B. burgdorferi sensu stricto
strain. The modified medium did not enable the growth of a B. afzelii
strain. The protein profile and antigenic characteristic of the
expressed proteins in the modified medium were studied with
satisfactory results. These results suggest the modified medium as an
alternative for the cultivation of Borrelia strains, with some
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modified medium, prepared in the laboratory using the constituents of
Barbour-Stonner-Kelly (BSK) medium and medium 199 as base, for the
culture of Borrelia strains, comparing the growth of individual strains
in this medium and in the BSK-H medium, and the protein profile and
antigenic characteristics of Borrelia proteins expressed in these
media. A qualitative evaluation of growth of Borrelia species was made
with acceptable results (morphology and motility), but during a
quantitative evaluation using the three main genospecies of Borrelia,
the better results were obtained with a B. burgdorferi sensu stricto
strain. The modified medium did not enable the growth of a B. afzelii
strain. The protein profile and antigenic characteristic of the
expressed proteins in the modified medium were studied with
satisfactory results. These results suggest the modified medium as an
alternative for the cultivation of Borrelia strains, with some
limitations, in poorly-resourced laboratories.</description><subject>Borrelia - new medium - BSK-H medium - proteins</subject><issn>1678-8060</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>RBI</sourceid><recordid>eNqVi00KwjAQRrNQsP7cYS5QSa20XVsq7nVfxjbRkaQjk0bw9grqAYQPHu_BN1FJVpRVWulCz9Q8hJvWmzIvtonC45VlhJq9jwN1OBIPkELzQBc_whYQPPdkyfTQRTdGMeBNT9GDZYEdixhHCOcol57FGiEIZggRHI68VFOLLpjVlwu13jen-pCeiR0Npr0LeZRn2wlh-4vcvafLrKzyvw8voZJPYQ</recordid><startdate>20080328</startdate><enddate>20080328</enddate><creator>Rodríguez, Islay</creator><creator>Lienhard, Reto</creator><creator>Gern, Lise</creator><creator>Veuve, Marie Colette</creator><creator>Jouda, Fatima</creator><creator>Siegrist, Hans H</creator><creator>Fernández, Carmen</creator><creator>Rodríguez, José Enrique</creator><general>Fundação Oswaldo Cruz, Fiocruz</general><scope>RBI</scope></search><sort><creationdate>20080328</creationdate><title>Short Communication - Evaluation of a modified culture medium for Borrelia burgdorferi sensu lato</title><author>Rodríguez, Islay ; Lienhard, Reto ; Gern, Lise ; Veuve, Marie Colette ; Jouda, Fatima ; Siegrist, Hans H ; Fernández, Carmen ; Rodríguez, José Enrique</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-bioline_primary_cria_bioline_oc_oc071783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Borrelia - new medium - BSK-H medium - proteins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rodríguez, Islay</creatorcontrib><creatorcontrib>Lienhard, Reto</creatorcontrib><creatorcontrib>Gern, Lise</creatorcontrib><creatorcontrib>Veuve, Marie Colette</creatorcontrib><creatorcontrib>Jouda, Fatima</creatorcontrib><creatorcontrib>Siegrist, Hans H</creatorcontrib><creatorcontrib>Fernández, Carmen</creatorcontrib><creatorcontrib>Rodríguez, José Enrique</creatorcontrib><collection>Bioline International</collection><jtitle>Memórias do Instituto Oswaldo Cruz</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rodríguez, Islay</au><au>Lienhard, Reto</au><au>Gern, Lise</au><au>Veuve, Marie Colette</au><au>Jouda, Fatima</au><au>Siegrist, Hans H</au><au>Fernández, Carmen</au><au>Rodríguez, José Enrique</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Short Communication - Evaluation of a modified culture medium for Borrelia burgdorferi sensu lato</atitle><jtitle>Memórias do Instituto Oswaldo Cruz</jtitle><date>2008-03-28</date><risdate>2008</risdate><volume>102</volume><issue>8</issue><issn>1678-8060</issn><abstract>The aim of the present study was to assess the possible use of a
modified medium, prepared in the laboratory using the constituents of
Barbour-Stonner-Kelly (BSK) medium and medium 199 as base, for the
culture of Borrelia strains, comparing the growth of individual strains
in this medium and in the BSK-H medium, and the protein profile and
antigenic characteristics of Borrelia proteins expressed in these
media. A qualitative evaluation of growth of Borrelia species was made
with acceptable results (morphology and motility), but during a
quantitative evaluation using the three main genospecies of Borrelia,
the better results were obtained with a B. burgdorferi sensu stricto
strain. The modified medium did not enable the growth of a B. afzelii
strain. The protein profile and antigenic characteristic of the
expressed proteins in the modified medium were studied with
satisfactory results. These results suggest the modified medium as an
alternative for the cultivation of Borrelia strains, with some
limitations, in poorly-resourced laboratories.</abstract><pub>Fundação Oswaldo Cruz, Fiocruz</pub></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1678-8060 |
| ispartof | Memórias do Instituto Oswaldo Cruz, 2008-03, Vol.102 (8) |
| issn | 1678-8060 |
| language | eng |
| recordid | cdi_bioline_primary_cria_bioline_oc_oc07178 |
| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Bioline International |
| subjects | Borrelia - new medium - BSK-H medium - proteins |
| title | Short Communication - Evaluation of a modified culture medium for Borrelia burgdorferi sensu lato |
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