Special Article - The effects of biocides (antiseptics and disinfectants) on the endospores of Rhinosporidium seeberi
No data exists on the activity of biocides (antiseptics and disinfectants) on Rhinosporidium seeberi that causes rhinosporidiosis in humans and animals. On account of the inability to culture R. seeberi, in vitro , dyes were used to assess the morphological integrity and viability of biocide-treated...
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| creator | Arseculeratne SN, Atapattu DN, Balasooriya P, Fernando R |
| description | No data exists on the activity of biocides (antiseptics and
disinfectants) on Rhinosporidium seeberi that causes rhinosporidiosis
in humans and animals. On account of the inability to culture R.
seeberi, in vitro , dyes were used to assess the morphological
integrity and viability of biocide-treated endospores that are
considered to be the infective stage of this pathogen. Evan's Blue
(EvB) identifies the morphological integrity of the endospores while
MTT (3-[4, 5-dimethylthiazol-2yl]-2, 5-diphenyl tetrazolium bromide)
identifies metabolic activity through its reduction by cellular
dehydrogenases to microscopically visible deposits of insoluble
formazan. MTT-negativity has earlier been shown to correlate with
absence of growth of yeast and mycelial fungi in culture and could thus
indicate the loss of viability of MTT-negative rhinosporidial
endospores. Hydrogen peroxide, glutaraldehyde, chloroxylenol,
chlorhexidine, cetrimide, thimerosal, 70% ethanol, iodine in 70%
ethanol, 10% formalin, povidone-iodine, sodium azide and silver nitrate
were tested on freshly-harvested endospores and all biocides caused
metabolic inactivation with or without altered structural integrity as
shown by absence of MTT-staining after 3, 24 or 36 hour after exposure,
while EvB stained only the endospores treated with sodium azide,
ethanol, thimerosal, chloroxylenol, glutaraldehyde and hydrogen
peroxide. With clinically useful biocides - chlorhexidine,
cetrimide-chlorhexidine, 70% ethanol, povidone-iodine and silver
nitrate, a total period of exposure of endospores to the biocide, for
seven minutes, produced metabolic inactivation of the endospores.
Anti-rhinosporidial antiseptics that could be used in surgery on
rhinosporidial patients include povidone-iodine in nasal packs for
nasal and naso-pharyngeal surgery, chlorhexidine and
cetrimide-chlorhexidine on the skin, while povidone-iodine and silver
nitrate could have application in ocular rhinosporidiosis. |
| format | Article |
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disinfectants) on Rhinosporidium seeberi that causes rhinosporidiosis
in humans and animals. On account of the inability to culture R.
seeberi, in vitro , dyes were used to assess the morphological
integrity and viability of biocide-treated endospores that are
considered to be the infective stage of this pathogen. Evan's Blue
(EvB) identifies the morphological integrity of the endospores while
MTT (3-[4, 5-dimethylthiazol-2yl]-2, 5-diphenyl tetrazolium bromide)
identifies metabolic activity through its reduction by cellular
dehydrogenases to microscopically visible deposits of insoluble
formazan. MTT-negativity has earlier been shown to correlate with
absence of growth of yeast and mycelial fungi in culture and could thus
indicate the loss of viability of MTT-negative rhinosporidial
endospores. Hydrogen peroxide, glutaraldehyde, chloroxylenol,
chlorhexidine, cetrimide, thimerosal, 70% ethanol, iodine in 70%
ethanol, 10% formalin, povidone-iodine, sodium azide and silver nitrate
were tested on freshly-harvested endospores and all biocides caused
metabolic inactivation with or without altered structural integrity as
shown by absence of MTT-staining after 3, 24 or 36 hour after exposure,
while EvB stained only the endospores treated with sodium azide,
ethanol, thimerosal, chloroxylenol, glutaraldehyde and hydrogen
peroxide. With clinically useful biocides - chlorhexidine,
cetrimide-chlorhexidine, 70% ethanol, povidone-iodine and silver
nitrate, a total period of exposure of endospores to the biocide, for
seven minutes, produced metabolic inactivation of the endospores.
Anti-rhinosporidial antiseptics that could be used in surgery on
rhinosporidial patients include povidone-iodine in nasal packs for
nasal and naso-pharyngeal surgery, chlorhexidine and
cetrimide-chlorhexidine on the skin, while povidone-iodine and silver
nitrate could have application in ocular rhinosporidiosis.</description><identifier>ISSN: 0255-0857</identifier><language>eng</language><publisher>Medknow Publications on behalf of Indian Association of Medical Microbiology</publisher><subject>Rhinosporidium seeberi, sensitivity, biocides</subject><ispartof>Indian journal of medical microbiology, 2006-06, Vol.24 (2)</ispartof><rights>Copyright 2006 Indian Journal of Medical Microbiology.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,79169</link.rule.ids></links><search><creatorcontrib>Arseculeratne SN, Atapattu DN, Balasooriya P, Fernando R</creatorcontrib><title>Special Article - The effects of biocides (antiseptics and disinfectants) on the endospores of Rhinosporidium seeberi</title><title>Indian journal of medical microbiology</title><description>No data exists on the activity of biocides (antiseptics and
disinfectants) on Rhinosporidium seeberi that causes rhinosporidiosis
in humans and animals. On account of the inability to culture R.
seeberi, in vitro , dyes were used to assess the morphological
integrity and viability of biocide-treated endospores that are
considered to be the infective stage of this pathogen. Evan's Blue
(EvB) identifies the morphological integrity of the endospores while
MTT (3-[4, 5-dimethylthiazol-2yl]-2, 5-diphenyl tetrazolium bromide)
identifies metabolic activity through its reduction by cellular
dehydrogenases to microscopically visible deposits of insoluble
formazan. MTT-negativity has earlier been shown to correlate with
absence of growth of yeast and mycelial fungi in culture and could thus
indicate the loss of viability of MTT-negative rhinosporidial
endospores. Hydrogen peroxide, glutaraldehyde, chloroxylenol,
chlorhexidine, cetrimide, thimerosal, 70% ethanol, iodine in 70%
ethanol, 10% formalin, povidone-iodine, sodium azide and silver nitrate
were tested on freshly-harvested endospores and all biocides caused
metabolic inactivation with or without altered structural integrity as
shown by absence of MTT-staining after 3, 24 or 36 hour after exposure,
while EvB stained only the endospores treated with sodium azide,
ethanol, thimerosal, chloroxylenol, glutaraldehyde and hydrogen
peroxide. With clinically useful biocides - chlorhexidine,
cetrimide-chlorhexidine, 70% ethanol, povidone-iodine and silver
nitrate, a total period of exposure of endospores to the biocide, for
seven minutes, produced metabolic inactivation of the endospores.
Anti-rhinosporidial antiseptics that could be used in surgery on
rhinosporidial patients include povidone-iodine in nasal packs for
nasal and naso-pharyngeal surgery, chlorhexidine and
cetrimide-chlorhexidine on the skin, while povidone-iodine and silver
nitrate could have application in ocular rhinosporidiosis.</description><subject>Rhinosporidium seeberi, sensitivity, biocides</subject><issn>0255-0857</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>RBI</sourceid><recordid>eNqVjcsKwjAQRbNQsD7-YZa6qAS1PpYiimt1X9JkiiNpUjLtwr83LfoBwsDlPg4zEIlcZVkq99luJMbMLxn95rBJRHuvUZOycAwNaYuQwuOJgGWJumHwJRTkNRlkmCvXEGMddwzKGTDE5LpdLHgB3kHToc54rn3Anr49yfWWDLUVMGKBgaZiWCrLOPvqRCwv58fpmsZnlhzmdaBKhXeuA6n8F1ZFPLmVq936b-ADDSVWVA</recordid><startdate>20060613</startdate><enddate>20060613</enddate><creator>Arseculeratne SN, Atapattu DN, Balasooriya P, Fernando R</creator><general>Medknow Publications on behalf of Indian Association of Medical Microbiology</general><scope>RBI</scope></search><sort><creationdate>20060613</creationdate><title>Special Article - The effects of biocides (antiseptics and disinfectants) on the endospores of Rhinosporidium seeberi</title><author>Arseculeratne SN, Atapattu DN, Balasooriya P, Fernando R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-bioline_primary_cria_bioline_mb_mb060273</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Rhinosporidium seeberi, sensitivity, biocides</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Arseculeratne SN, Atapattu DN, Balasooriya P, Fernando R</creatorcontrib><collection>Bioline International</collection><jtitle>Indian journal of medical microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Arseculeratne SN, Atapattu DN, Balasooriya P, Fernando R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Special Article - The effects of biocides (antiseptics and disinfectants) on the endospores of Rhinosporidium seeberi</atitle><jtitle>Indian journal of medical microbiology</jtitle><date>2006-06-13</date><risdate>2006</risdate><volume>24</volume><issue>2</issue><issn>0255-0857</issn><abstract>No data exists on the activity of biocides (antiseptics and
disinfectants) on Rhinosporidium seeberi that causes rhinosporidiosis
in humans and animals. On account of the inability to culture R.
seeberi, in vitro , dyes were used to assess the morphological
integrity and viability of biocide-treated endospores that are
considered to be the infective stage of this pathogen. Evan's Blue
(EvB) identifies the morphological integrity of the endospores while
MTT (3-[4, 5-dimethylthiazol-2yl]-2, 5-diphenyl tetrazolium bromide)
identifies metabolic activity through its reduction by cellular
dehydrogenases to microscopically visible deposits of insoluble
formazan. MTT-negativity has earlier been shown to correlate with
absence of growth of yeast and mycelial fungi in culture and could thus
indicate the loss of viability of MTT-negative rhinosporidial
endospores. Hydrogen peroxide, glutaraldehyde, chloroxylenol,
chlorhexidine, cetrimide, thimerosal, 70% ethanol, iodine in 70%
ethanol, 10% formalin, povidone-iodine, sodium azide and silver nitrate
were tested on freshly-harvested endospores and all biocides caused
metabolic inactivation with or without altered structural integrity as
shown by absence of MTT-staining after 3, 24 or 36 hour after exposure,
while EvB stained only the endospores treated with sodium azide,
ethanol, thimerosal, chloroxylenol, glutaraldehyde and hydrogen
peroxide. With clinically useful biocides - chlorhexidine,
cetrimide-chlorhexidine, 70% ethanol, povidone-iodine and silver
nitrate, a total period of exposure of endospores to the biocide, for
seven minutes, produced metabolic inactivation of the endospores.
Anti-rhinosporidial antiseptics that could be used in surgery on
rhinosporidial patients include povidone-iodine in nasal packs for
nasal and naso-pharyngeal surgery, chlorhexidine and
cetrimide-chlorhexidine on the skin, while povidone-iodine and silver
nitrate could have application in ocular rhinosporidiosis.</abstract><pub>Medknow Publications on behalf of Indian Association of Medical Microbiology</pub></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0255-0857 |
| ispartof | Indian journal of medical microbiology, 2006-06, Vol.24 (2) |
| issn | 0255-0857 |
| language | eng |
| recordid | cdi_bioline_primary_cria_bioline_mb_mb06027 |
| source | Bioline International; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Rhinosporidium seeberi, sensitivity, biocides |
| title | Special Article - The effects of biocides (antiseptics and disinfectants) on the endospores of Rhinosporidium seeberi |
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