Peptide research protocols: endothelin

Tantalizing new research frontiers are opening in peptide research, where the endothelins constitute an important class of signals that can be manipulated therapeutically to treat cardiovascular disease. In Peptide Research Protocols: Endothelin, a panel of multidisciplinary experts describes in det...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: Maguire, Janet J, Davenport, Anthony P
Format: Buch
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue
container_start_page
container_title
container_volume 206
creator Maguire, Janet J
Davenport, Anthony P
description Tantalizing new research frontiers are opening in peptide research, where the endothelins constitute an important class of signals that can be manipulated therapeutically to treat cardiovascular disease. In Peptide Research Protocols: Endothelin, a panel of multidisciplinary experts describes in detail readily reproducible methods to investigate all aspects of peptide research by focusing on the endothelin system-from its synthesis and metabolism, to its function in health and disease. These methods use state-of-the-art molecular techniques to quantify the expression of mRNA, here using both endothelin receptors and the endothelin-converting enzymes. They show how peptides, precursors, receptors, and synthetic enzymes can be localized and quantified in plasma, culture supernatants, tissue homogenate, and tissue sections using antibodies, and how radioligand binding techniques can provide additional information on receptor characterization. In vitro assays are presented to determine the function of peptides in isolated preparations, to characterize new receptor ligands, and to provide information on the tissue-specific processing of precursor peptides-all in the endothelin system. Several in vivo protocols illustrate the role of the endothelin peptides in healthy human individuals and describe animal models that can be used to predict the therapeutic potential of cardiovascular drugs that manipulate endothelin synthesis or function. Authoritative and timely, Peptide Research Protocols: Endothelin provides both a comprehensive introduction to endothelin biology and an up-to-date collection of proven techniques and experimental strategies for use in peptide research today.
doi_str_mv 10.1385/1592592899
format Book
fullrecord <record><control><sourceid>proquest_askew</sourceid><recordid>TN_cdi_askewsholts_vlebooks_9781592592890</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>EBC3037043</sourcerecordid><originalsourceid>FETCH-LOGICAL-a2822x-2d9c4b95acf650236cf9ce85705bf33b2b257bdd01a38a6de5bfd715647e64283</originalsourceid><addsrcrecordid>eNpFkEtLAzEQxyOi2GovHr3Ym3iozWPzmKOW-oCCgo9ryGZn27VLtyZr9eObumKHgWGG37z-hJwyesWEkWMmgSc3AHukv0v2SZ8aUFQACHlIesCFAgMcjsggxneajDOQBnrk7AnXbVXgMGBEF_xiuA5N2_imjifkoHR1xMFfPCZvt9OXyf1o9nj3MLmejRw3nH-PeAE-y0E6XypJ0ypfgkcjNZV5KUTOcy51XhSUOWGcKjCVC82kyjSqjBtxTC67wS4u8SsumrqNdlNj3jTLaEGb_89oYscdG9ehWs0x2C21PbzaYLSM2q0wdqdF6rjoOtJjH58YW_s72OOqDa6205uJoELTTCTyvCNL11g3D1W0r8-cMpH00oolPX8AZ-hnCw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>book</recordtype><pqid>EBC3037043</pqid></control><display><type>book</type><title>Peptide research protocols: endothelin</title><source>Springer Books</source><creator>Maguire, Janet J ; Davenport, Anthony P</creator><creatorcontrib>Maguire, Janet J ; Davenport, Anthony P ; SpringerLink (Online service)</creatorcontrib><description>Tantalizing new research frontiers are opening in peptide research, where the endothelins constitute an important class of signals that can be manipulated therapeutically to treat cardiovascular disease. In Peptide Research Protocols: Endothelin, a panel of multidisciplinary experts describes in detail readily reproducible methods to investigate all aspects of peptide research by focusing on the endothelin system-from its synthesis and metabolism, to its function in health and disease. These methods use state-of-the-art molecular techniques to quantify the expression of mRNA, here using both endothelin receptors and the endothelin-converting enzymes. They show how peptides, precursors, receptors, and synthetic enzymes can be localized and quantified in plasma, culture supernatants, tissue homogenate, and tissue sections using antibodies, and how radioligand binding techniques can provide additional information on receptor characterization. In vitro assays are presented to determine the function of peptides in isolated preparations, to characterize new receptor ligands, and to provide information on the tissue-specific processing of precursor peptides-all in the endothelin system. Several in vivo protocols illustrate the role of the endothelin peptides in healthy human individuals and describe animal models that can be used to predict the therapeutic potential of cardiovascular drugs that manipulate endothelin synthesis or function. Authoritative and timely, Peptide Research Protocols: Endothelin provides both a comprehensive introduction to endothelin biology and an up-to-date collection of proven techniques and experimental strategies for use in peptide research today.</description><edition>2002</edition><identifier>ISBN: 0896039935</identifier><identifier>ISBN: 9780896039933</identifier><identifier>ISBN: 9781617372919</identifier><identifier>ISBN: 1617372919</identifier><identifier>ISBN: 9781489938572</identifier><identifier>ISBN: 1489938575</identifier><identifier>EISBN: 1592592899</identifier><identifier>EISBN: 9781592592890</identifier><identifier>DOI: 10.1385/1592592899</identifier><identifier>OCLC: 923698929</identifier><language>eng</language><publisher>Totowa, NJ: Humana Press</publisher><subject>Endothelins ; Laboratory manuals ; Pharmacy</subject><creationdate>2002</creationdate><tpages>242</tpages><format>242</format><woscitedreferencessubscribed>false</woscitedreferencessubscribed><relation>Methods in Molecular Biology</relation></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1385/1592592899$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1385/1592592899$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>306,307,780,784,786,787,4045,27923,41440,42509</link.rule.ids></links><search><creatorcontrib>Maguire, Janet J</creatorcontrib><creatorcontrib>Davenport, Anthony P</creatorcontrib><creatorcontrib>SpringerLink (Online service)</creatorcontrib><title>Peptide research protocols: endothelin</title><description>Tantalizing new research frontiers are opening in peptide research, where the endothelins constitute an important class of signals that can be manipulated therapeutically to treat cardiovascular disease. In Peptide Research Protocols: Endothelin, a panel of multidisciplinary experts describes in detail readily reproducible methods to investigate all aspects of peptide research by focusing on the endothelin system-from its synthesis and metabolism, to its function in health and disease. These methods use state-of-the-art molecular techniques to quantify the expression of mRNA, here using both endothelin receptors and the endothelin-converting enzymes. They show how peptides, precursors, receptors, and synthetic enzymes can be localized and quantified in plasma, culture supernatants, tissue homogenate, and tissue sections using antibodies, and how radioligand binding techniques can provide additional information on receptor characterization. In vitro assays are presented to determine the function of peptides in isolated preparations, to characterize new receptor ligands, and to provide information on the tissue-specific processing of precursor peptides-all in the endothelin system. Several in vivo protocols illustrate the role of the endothelin peptides in healthy human individuals and describe animal models that can be used to predict the therapeutic potential of cardiovascular drugs that manipulate endothelin synthesis or function. Authoritative and timely, Peptide Research Protocols: Endothelin provides both a comprehensive introduction to endothelin biology and an up-to-date collection of proven techniques and experimental strategies for use in peptide research today.</description><subject>Endothelins</subject><subject>Laboratory manuals</subject><subject>Pharmacy</subject><isbn>0896039935</isbn><isbn>9780896039933</isbn><isbn>9781617372919</isbn><isbn>1617372919</isbn><isbn>9781489938572</isbn><isbn>1489938575</isbn><isbn>1592592899</isbn><isbn>9781592592890</isbn><fulltext>true</fulltext><rsrctype>book</rsrctype><creationdate>2002</creationdate><recordtype>book</recordtype><recordid>eNpFkEtLAzEQxyOi2GovHr3Ym3iozWPzmKOW-oCCgo9ryGZn27VLtyZr9eObumKHgWGG37z-hJwyesWEkWMmgSc3AHukv0v2SZ8aUFQACHlIesCFAgMcjsggxneajDOQBnrk7AnXbVXgMGBEF_xiuA5N2_imjifkoHR1xMFfPCZvt9OXyf1o9nj3MLmejRw3nH-PeAE-y0E6XypJ0ypfgkcjNZV5KUTOcy51XhSUOWGcKjCVC82kyjSqjBtxTC67wS4u8SsumrqNdlNj3jTLaEGb_89oYscdG9ehWs0x2C21PbzaYLSM2q0wdqdF6rjoOtJjH58YW_s72OOqDa6205uJoELTTCTyvCNL11g3D1W0r8-cMpH00oolPX8AZ-hnCw</recordid><startdate>2002</startdate><enddate>2002</enddate><creator>Maguire, Janet J</creator><creator>Davenport, Anthony P</creator><general>Humana Press</general><scope>FBQ</scope></search><sort><creationdate>2002</creationdate><title>Peptide research protocols</title><author>Maguire, Janet J ; Davenport, Anthony P</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a2822x-2d9c4b95acf650236cf9ce85705bf33b2b257bdd01a38a6de5bfd715647e64283</frbrgroupid><rsrctype>books</rsrctype><prefilter>books</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Endothelins</topic><topic>Laboratory manuals</topic><topic>Pharmacy</topic><toplevel>online_resources</toplevel><creatorcontrib>Maguire, Janet J</creatorcontrib><creatorcontrib>Davenport, Anthony P</creatorcontrib><creatorcontrib>SpringerLink (Online service)</creatorcontrib><collection>AGRIS</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Maguire, Janet J</au><au>Davenport, Anthony P</au><aucorp>SpringerLink (Online service)</aucorp><format>book</format><genre>book</genre><ristype>BOOK</ristype><btitle>Peptide research protocols: endothelin</btitle><seriestitle>Methods in Molecular Biology</seriestitle><date>2002</date><risdate>2002</risdate><volume>206</volume><isbn>0896039935</isbn><isbn>9780896039933</isbn><isbn>9781617372919</isbn><isbn>1617372919</isbn><isbn>9781489938572</isbn><isbn>1489938575</isbn><eisbn>1592592899</eisbn><eisbn>9781592592890</eisbn><abstract>Tantalizing new research frontiers are opening in peptide research, where the endothelins constitute an important class of signals that can be manipulated therapeutically to treat cardiovascular disease. In Peptide Research Protocols: Endothelin, a panel of multidisciplinary experts describes in detail readily reproducible methods to investigate all aspects of peptide research by focusing on the endothelin system-from its synthesis and metabolism, to its function in health and disease. These methods use state-of-the-art molecular techniques to quantify the expression of mRNA, here using both endothelin receptors and the endothelin-converting enzymes. They show how peptides, precursors, receptors, and synthetic enzymes can be localized and quantified in plasma, culture supernatants, tissue homogenate, and tissue sections using antibodies, and how radioligand binding techniques can provide additional information on receptor characterization. In vitro assays are presented to determine the function of peptides in isolated preparations, to characterize new receptor ligands, and to provide information on the tissue-specific processing of precursor peptides-all in the endothelin system. Several in vivo protocols illustrate the role of the endothelin peptides in healthy human individuals and describe animal models that can be used to predict the therapeutic potential of cardiovascular drugs that manipulate endothelin synthesis or function. Authoritative and timely, Peptide Research Protocols: Endothelin provides both a comprehensive introduction to endothelin biology and an up-to-date collection of proven techniques and experimental strategies for use in peptide research today.</abstract><cop>Totowa, NJ</cop><pub>Humana Press</pub><doi>10.1385/1592592899</doi><oclcid>923698929</oclcid><tpages>242</tpages><edition>2002</edition></addata></record>
fulltext fulltext
identifier ISBN: 0896039935
ispartof
issn
language eng
recordid cdi_askewsholts_vlebooks_9781592592890
source Springer Books
subjects Endothelins
Laboratory manuals
Pharmacy
title Peptide research protocols: endothelin
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-14T08%3A18%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_askew&rft_val_fmt=info:ofi/fmt:kev:mtx:book&rft.genre=book&rft.btitle=Peptide%20research%20protocols:%20endothelin&rft.au=Maguire,%20Janet%20J&rft.aucorp=SpringerLink%20(Online%20service)&rft.date=2002&rft.volume=206&rft.isbn=0896039935&rft.isbn_list=9780896039933&rft.isbn_list=9781617372919&rft.isbn_list=1617372919&rft.isbn_list=9781489938572&rft.isbn_list=1489938575&rft_id=info:doi/10.1385/1592592899&rft_dat=%3Cproquest_askew%3EEBC3037043%3C/proquest_askew%3E%3Curl%3E%3C/url%3E&rft.eisbn=1592592899&rft.eisbn_list=9781592592890&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=EBC3037043&rft_id=info:pmid/&rfr_iscdi=true