Ice Formation in Model Biological Membranes in the Presence of Cryoprotectors
Nuclear Instruments & Methods in Physics Research A 448 (2000) 225-260 Ice formation in model biological membranes is studied by SAXS and WAXS in the presence of cryoprotectors: dimethyl sulfoxide and glycerol. Three types of phospholipid membranes: DPPC, DMPC, DSPC are chosen for the investigat...
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| creator | Kiselev, M. A Lesieur, P Kisselev, A. M Ollivon, M |
| description | Nuclear Instruments & Methods in Physics Research A 448 (2000)
225-260 Ice formation in model biological membranes is studied by SAXS and WAXS in
the presence of cryoprotectors: dimethyl sulfoxide and glycerol. Three types of
phospholipid membranes: DPPC, DMPC, DSPC are chosen for the investigation as
well-studied model biological membranes. A special cryostat is used for sample
cooling from 14.1C to -55.4C. The ice formation is only detected by WAXS in
binary phospholipid/water and ternary phospholipid/cryoprotector/water systems
in the condition of excess solvent. Ice formation in a binary
phospholipid/water system creates an abrupt decrease of the membrane repeat
distance by delta-d, so-called ice-induced dehydration of intermembrane space.
The value of delta-d decreases as the cryoprotector concentration increases.
The formation of ice does not influence the membrane structure (delta-d = 0)
for cryoprotector mole fractions higher than 0.05. |
| doi_str_mv | 10.48550/arxiv.physics/0108023 |
| format | Article |
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225-260 Ice formation in model biological membranes is studied by SAXS and WAXS in
the presence of cryoprotectors: dimethyl sulfoxide and glycerol. Three types of
phospholipid membranes: DPPC, DMPC, DSPC are chosen for the investigation as
well-studied model biological membranes. A special cryostat is used for sample
cooling from 14.1C to -55.4C. The ice formation is only detected by WAXS in
binary phospholipid/water and ternary phospholipid/cryoprotector/water systems
in the condition of excess solvent. Ice formation in a binary
phospholipid/water system creates an abrupt decrease of the membrane repeat
distance by delta-d, so-called ice-induced dehydration of intermembrane space.
The value of delta-d decreases as the cryoprotector concentration increases.
The formation of ice does not influence the membrane structure (delta-d = 0)
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225-260 Ice formation in model biological membranes is studied by SAXS and WAXS in
the presence of cryoprotectors: dimethyl sulfoxide and glycerol. Three types of
phospholipid membranes: DPPC, DMPC, DSPC are chosen for the investigation as
well-studied model biological membranes. A special cryostat is used for sample
cooling from 14.1C to -55.4C. The ice formation is only detected by WAXS in
binary phospholipid/water and ternary phospholipid/cryoprotector/water systems
in the condition of excess solvent. Ice formation in a binary
phospholipid/water system creates an abrupt decrease of the membrane repeat
distance by delta-d, so-called ice-induced dehydration of intermembrane space.
The value of delta-d decreases as the cryoprotector concentration increases.
The formation of ice does not influence the membrane structure (delta-d = 0)
for cryoprotector mole fractions higher than 0.05.</description><subject>Physics - Biological Physics</subject><subject>Physics - Chemical Physics</subject><subject>Physics - Medical Physics</subject><subject>Physics - Soft Condensed Matter</subject><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>GOX</sourceid><recordid>eNqNjr0KwjAUhbM4iPoKcgdX29Ra6Gyx6FBwcC8x3tpAkltugti394c-gNMZzvk4nxDrTCb7sihkqvhlnsnQj8HokMpMlnKXz0Vz1gg1sVPRkAfjoaE7WjgYsvQwWllo0N1YeQzfNvYIF8aA_sNRBxWPNDBF1JE4LMWsUzbgasqF2NTHa3Xa_t7bgY1TPLaTRTtZ5H_O3qzvQw8</recordid><startdate>20010814</startdate><enddate>20010814</enddate><creator>Kiselev, M. A</creator><creator>Lesieur, P</creator><creator>Kisselev, A. M</creator><creator>Ollivon, M</creator><scope>GOX</scope></search><sort><creationdate>20010814</creationdate><title>Ice Formation in Model Biological Membranes in the Presence of Cryoprotectors</title><author>Kiselev, M. A ; Lesieur, P ; Kisselev, A. M ; Ollivon, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-arxiv_primary_physics_01080233</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Physics - Biological Physics</topic><topic>Physics - Chemical Physics</topic><topic>Physics - Medical Physics</topic><topic>Physics - Soft Condensed Matter</topic><toplevel>online_resources</toplevel><creatorcontrib>Kiselev, M. A</creatorcontrib><creatorcontrib>Lesieur, P</creatorcontrib><creatorcontrib>Kisselev, A. M</creatorcontrib><creatorcontrib>Ollivon, M</creatorcontrib><collection>arXiv.org</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Kiselev, M. A</au><au>Lesieur, P</au><au>Kisselev, A. M</au><au>Ollivon, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ice Formation in Model Biological Membranes in the Presence of Cryoprotectors</atitle><date>2001-08-14</date><risdate>2001</risdate><abstract>Nuclear Instruments & Methods in Physics Research A 448 (2000)
225-260 Ice formation in model biological membranes is studied by SAXS and WAXS in
the presence of cryoprotectors: dimethyl sulfoxide and glycerol. Three types of
phospholipid membranes: DPPC, DMPC, DSPC are chosen for the investigation as
well-studied model biological membranes. A special cryostat is used for sample
cooling from 14.1C to -55.4C. The ice formation is only detected by WAXS in
binary phospholipid/water and ternary phospholipid/cryoprotector/water systems
in the condition of excess solvent. Ice formation in a binary
phospholipid/water system creates an abrupt decrease of the membrane repeat
distance by delta-d, so-called ice-induced dehydration of intermembrane space.
The value of delta-d decreases as the cryoprotector concentration increases.
The formation of ice does not influence the membrane structure (delta-d = 0)
for cryoprotector mole fractions higher than 0.05.</abstract><doi>10.48550/arxiv.physics/0108023</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Physics - Biological Physics Physics - Chemical Physics Physics - Medical Physics Physics - Soft Condensed Matter |
| title | Ice Formation in Model Biological Membranes in the Presence of Cryoprotectors |
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