Active Healing of Microtubule-Motor Networks

Cytoskeletal networks have a self-healing property where networks can repair defects to maintain structural integrity. However, both the mechanisms and dynamics of healing remain largely unknown. Here we report an unexplored healing mechanism in microtubule-motor networks by active crosslinking. We directly generate network cracks using a light-controlled microtubule-motor system, and observe that the cracks can self-heal. Combining theory and experiment, we find that the networks must overcome internal elastic resistance in order to heal cracks, giving rise to a bifurcation of dynamics dependent on the initial opening angle of the crack: the crack heals below a critical angle and opens up at larger angles. Simulation of a continuum model reproduces the bifurcation dynamics, revealing the importance of a boundary layer where free motors and microtubules can actively crosslink and thereby heal the crack. We also formulate a simple elastic-rod model that can qualitatively predict the critical angle, which is found to be tunable by two dimensionless geometric parameters, the ratio of the boundary layer and network width, and the aspect ratio of the network. Our results provide a new framework for understanding healing in cytoskeletal networks and designing self-healable biomaterials.