Nanoscopic distribution of VAChT and VGLUT3 in striatal cholinergic varicosities suggests colocalization and segregation of the two transporters in synaptic vesicles
Striatal cholinergic interneurons (CINs) use acetylcholine (ACh) and glutamate (Glut) to regulate the striatal network since they express vesicular transporters for ACh (VAChT) and Glut (VGLUT3). However, whether ACh and Glut are released simultaneously and/or independently from cholinergic varicosi...
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| creator | Cristofari, Paola Desplanque, Mazarine Poirel, Odile Hébert, Alison Dumas, Sylvie Herzog, Etienne Danglot, Lydia Geny, David Gilles, Jean-François Geeverding, Audrey Bolte, Susanne Canette, Alexis Trichet, Michaël Fabre, Véronique Daumas, Stéphanie Pietrancosta, Nicolas Salah El Mestikawy Bernard, Véronique |
| description | Striatal cholinergic interneurons (CINs) use acetylcholine (ACh) and glutamate (Glut) to regulate the striatal network since they express vesicular transporters for ACh (VAChT) and Glut (VGLUT3). However, whether ACh and Glut are released simultaneously and/or independently from cholinergic varicosities is an open question. The answer to that question requires the multichannel detection of vesicular transporters at the level of single synaptic vesicle (SV). Here, we used super-resolution STimulated Emission Depletion microscopy (STED) to characterize and quantify the distribution of VAChT and VGLUT3 in CINs SVs. Nearest-neighbor distances analysis between VAChT and VGLUT3-immunofluorescent spots revealed that 34% of CINs SVs contain both VAChT and VGLUT3. In addition, 40% of SVs expressed only VAChT while 26% of SVs contain only VGLUT3. These results suggest that SVs from CINs have the potential to store simultaneously or independently ACh and/or Glut. Overall, these morphological findings support the notion that CINs varicosities can signal with either ACh or Glut or both with an unexpected level of complexity. |
| doi_str_mv | 10.48550/arxiv.2210.02728 |
| format | Article |
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However, whether ACh and Glut are released simultaneously and/or independently from cholinergic varicosities is an open question. The answer to that question requires the multichannel detection of vesicular transporters at the level of single synaptic vesicle (SV). Here, we used super-resolution STimulated Emission Depletion microscopy (STED) to characterize and quantify the distribution of VAChT and VGLUT3 in CINs SVs. Nearest-neighbor distances analysis between VAChT and VGLUT3-immunofluorescent spots revealed that 34% of CINs SVs contain both VAChT and VGLUT3. In addition, 40% of SVs expressed only VAChT while 26% of SVs contain only VGLUT3. These results suggest that SVs from CINs have the potential to store simultaneously or independently ACh and/or Glut. Overall, these morphological findings support the notion that CINs varicosities can signal with either ACh or Glut or both with an unexpected level of complexity.</description><identifier>EISSN: 2331-8422</identifier><identifier>DOI: 10.48550/arxiv.2210.02728</identifier><language>eng</language><publisher>Ithaca: Cornell University Library, arXiv.org</publisher><subject>Cholinergics ; Depletion ; Quantitative Biology - Neurons and Cognition ; Questions ; Stimulated emission</subject><ispartof>arXiv.org, 2022-10</ispartof><rights>2022. This work is published under http://arxiv.org/licenses/nonexclusive-distrib/1.0/ (the “License”). 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Desplanque, Mazarine ; Poirel, Odile ; Hébert, Alison ; Dumas, Sylvie ; Herzog, Etienne ; Danglot, Lydia ; Geny, David ; Gilles, Jean-François ; Geeverding, Audrey ; Bolte, Susanne ; Canette, Alexis ; Trichet, Michaël ; Fabre, Véronique ; Daumas, Stéphanie ; Pietrancosta, Nicolas ; Salah El Mestikawy ; Bernard, Véronique</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a528-cacebe21292e38caeeda32d1c551cac4a5432d664bbd8a353688e00ce3ddb54f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Cholinergics</topic><topic>Depletion</topic><topic>Quantitative Biology - Neurons and Cognition</topic><topic>Questions</topic><topic>Stimulated emission</topic><toplevel>online_resources</toplevel><creatorcontrib>Cristofari, Paola</creatorcontrib><creatorcontrib>Desplanque, Mazarine</creatorcontrib><creatorcontrib>Poirel, Odile</creatorcontrib><creatorcontrib>Hébert, Alison</creatorcontrib><creatorcontrib>Dumas, Sylvie</creatorcontrib><creatorcontrib>Herzog, Etienne</creatorcontrib><creatorcontrib>Danglot, Lydia</creatorcontrib><creatorcontrib>Geny, David</creatorcontrib><creatorcontrib>Gilles, Jean-François</creatorcontrib><creatorcontrib>Geeverding, Audrey</creatorcontrib><creatorcontrib>Bolte, Susanne</creatorcontrib><creatorcontrib>Canette, Alexis</creatorcontrib><creatorcontrib>Trichet, Michaël</creatorcontrib><creatorcontrib>Fabre, Véronique</creatorcontrib><creatorcontrib>Daumas, Stéphanie</creatorcontrib><creatorcontrib>Pietrancosta, Nicolas</creatorcontrib><creatorcontrib>Salah El Mestikawy</creatorcontrib><creatorcontrib>Bernard, Véronique</creatorcontrib><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Engineering Database</collection><collection>Publicly Available Content Database (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering collection</collection><collection>arXiv Quantitative Biology</collection><collection>arXiv.org</collection><jtitle>arXiv.org</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cristofari, Paola</au><au>Desplanque, Mazarine</au><au>Poirel, Odile</au><au>Hébert, Alison</au><au>Dumas, Sylvie</au><au>Herzog, Etienne</au><au>Danglot, Lydia</au><au>Geny, David</au><au>Gilles, Jean-François</au><au>Geeverding, Audrey</au><au>Bolte, Susanne</au><au>Canette, Alexis</au><au>Trichet, Michaël</au><au>Fabre, Véronique</au><au>Daumas, Stéphanie</au><au>Pietrancosta, Nicolas</au><au>Salah El Mestikawy</au><au>Bernard, Véronique</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nanoscopic distribution of VAChT and VGLUT3 in striatal cholinergic varicosities suggests colocalization and segregation of the two transporters in synaptic vesicles</atitle><jtitle>arXiv.org</jtitle><date>2022-10-06</date><risdate>2022</risdate><eissn>2331-8422</eissn><abstract>Striatal cholinergic interneurons (CINs) use acetylcholine (ACh) and glutamate (Glut) to regulate the striatal network since they express vesicular transporters for ACh (VAChT) and Glut (VGLUT3). However, whether ACh and Glut are released simultaneously and/or independently from cholinergic varicosities is an open question. The answer to that question requires the multichannel detection of vesicular transporters at the level of single synaptic vesicle (SV). Here, we used super-resolution STimulated Emission Depletion microscopy (STED) to characterize and quantify the distribution of VAChT and VGLUT3 in CINs SVs. Nearest-neighbor distances analysis between VAChT and VGLUT3-immunofluorescent spots revealed that 34% of CINs SVs contain both VAChT and VGLUT3. In addition, 40% of SVs expressed only VAChT while 26% of SVs contain only VGLUT3. These results suggest that SVs from CINs have the potential to store simultaneously or independently ACh and/or Glut. Overall, these morphological findings support the notion that CINs varicosities can signal with either ACh or Glut or both with an unexpected level of complexity.</abstract><cop>Ithaca</cop><pub>Cornell University Library, arXiv.org</pub><doi>10.48550/arxiv.2210.02728</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Cholinergics Depletion Quantitative Biology - Neurons and Cognition Questions Stimulated emission |
| title | Nanoscopic distribution of VAChT and VGLUT3 in striatal cholinergic varicosities suggests colocalization and segregation of the two transporters in synaptic vesicles |
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