An Arduino-based, low-cost imaging incubator for extended live cell imaging

In order to image live cells for prolonged periods of time, an Arduino-based, low-cost imaging incubator was constructed. The imaging incubator keeps cells viable by controlling for temperature and CO2 in order to maintain physiological conditions for cells during imaging. All devices and parts empl...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:arXiv.org 2022-05
Hauptverfasser: Rossi, Vincent M, Davidson, Katherine C, Moore, Lauren E
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page
container_issue
container_start_page
container_title arXiv.org
container_volume
creator Rossi, Vincent M
Davidson, Katherine C
Moore, Lauren E
description In order to image live cells for prolonged periods of time, an Arduino-based, low-cost imaging incubator was constructed. The imaging incubator keeps cells viable by controlling for temperature and CO2 in order to maintain physiological conditions for cells during imaging. All devices and parts employed in the build were typical maker-type components in order to minimize the cost of the imaging incubator. The imaging incubator allows for real-time imaging of live cells exposed to any desired perturbation or stimulus. As a proof of the system's functionality, cells are imaged over 24 hours while remaining viable in the imaging incubator.
doi_str_mv 10.48550/arxiv.2206.02542
format Article
fullrecord <record><control><sourceid>proquest_arxiv</sourceid><recordid>TN_cdi_arxiv_primary_2206_02542</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2673710269</sourcerecordid><originalsourceid>FETCH-LOGICAL-a529-6541a116b30a037c57ff0a79e14154d18fd74acd8fffe8212ec5a5d16bdef4423</originalsourceid><addsrcrecordid>eNo1j8tOwzAQRS0kJKrSD2CFJba42GM7j2VU8RKV2HQfOfG4chXi4iSl_D2mhcXobs7MnUPIjeBLVWjNH0w8-sMSgGdLDlrBBZmBlIIVCuCKLIZhxzmHLAet5Yy8VT2top18H1hjBrT3tAtfrA3DSP2H2fp-S33fTo0ZQ6QuDR5H7C1a2vkD0ha77h-8JpfOdAMu_nJONk-Pm9ULW78_v66qNTMaSpZpJYwQWSO54TJvde4cN3mJQgmtrCiczZVpbeGcwwIEYKuNtmnBolMK5Jzcns-eTOt9TPXxu_41rk_Gibg7E_sYPiccxnoXptinn-okLnOR_Ev5A-p8WJM</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2673710269</pqid></control><display><type>article</type><title>An Arduino-based, low-cost imaging incubator for extended live cell imaging</title><source>arXiv.org</source><source>Free E- Journals</source><creator>Rossi, Vincent M ; Davidson, Katherine C ; Moore, Lauren E</creator><creatorcontrib>Rossi, Vincent M ; Davidson, Katherine C ; Moore, Lauren E</creatorcontrib><description>In order to image live cells for prolonged periods of time, an Arduino-based, low-cost imaging incubator was constructed. The imaging incubator keeps cells viable by controlling for temperature and CO2 in order to maintain physiological conditions for cells during imaging. All devices and parts employed in the build were typical maker-type components in order to minimize the cost of the imaging incubator. The imaging incubator allows for real-time imaging of live cells exposed to any desired perturbation or stimulus. As a proof of the system's functionality, cells are imaged over 24 hours while remaining viable in the imaging incubator.</description><identifier>EISSN: 2331-8422</identifier><identifier>DOI: 10.48550/arxiv.2206.02542</identifier><language>eng</language><publisher>Ithaca: Cornell University Library, arXiv.org</publisher><subject>Imaging ; Low cost ; Perturbation ; Physics - Instrumentation and Detectors ; Physics - Medical Physics</subject><ispartof>arXiv.org, 2022-05</ispartof><rights>2022. This work is published under http://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>http://creativecommons.org/licenses/by-nc-nd/4.0</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>228,230,780,784,885,27925</link.rule.ids><backlink>$$Uhttps://doi.org/10.48550/arXiv.2206.02542$$DView paper in arXiv$$Hfree_for_read</backlink><backlink>$$Uhttps://doi.org/10.1364/AO.460443$$DView published paper (Access to full text may be restricted)$$Hfree_for_read</backlink></links><search><creatorcontrib>Rossi, Vincent M</creatorcontrib><creatorcontrib>Davidson, Katherine C</creatorcontrib><creatorcontrib>Moore, Lauren E</creatorcontrib><title>An Arduino-based, low-cost imaging incubator for extended live cell imaging</title><title>arXiv.org</title><description>In order to image live cells for prolonged periods of time, an Arduino-based, low-cost imaging incubator was constructed. The imaging incubator keeps cells viable by controlling for temperature and CO2 in order to maintain physiological conditions for cells during imaging. All devices and parts employed in the build were typical maker-type components in order to minimize the cost of the imaging incubator. The imaging incubator allows for real-time imaging of live cells exposed to any desired perturbation or stimulus. As a proof of the system's functionality, cells are imaged over 24 hours while remaining viable in the imaging incubator.</description><subject>Imaging</subject><subject>Low cost</subject><subject>Perturbation</subject><subject>Physics - Instrumentation and Detectors</subject><subject>Physics - Medical Physics</subject><issn>2331-8422</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GOX</sourceid><recordid>eNo1j8tOwzAQRS0kJKrSD2CFJba42GM7j2VU8RKV2HQfOfG4chXi4iSl_D2mhcXobs7MnUPIjeBLVWjNH0w8-sMSgGdLDlrBBZmBlIIVCuCKLIZhxzmHLAet5Yy8VT2top18H1hjBrT3tAtfrA3DSP2H2fp-S33fTo0ZQ6QuDR5H7C1a2vkD0ha77h-8JpfOdAMu_nJONk-Pm9ULW78_v66qNTMaSpZpJYwQWSO54TJvde4cN3mJQgmtrCiczZVpbeGcwwIEYKuNtmnBolMK5Jzcns-eTOt9TPXxu_41rk_Gibg7E_sYPiccxnoXptinn-okLnOR_Ev5A-p8WJM</recordid><startdate>20220528</startdate><enddate>20220528</enddate><creator>Rossi, Vincent M</creator><creator>Davidson, Katherine C</creator><creator>Moore, Lauren E</creator><general>Cornell University Library, arXiv.org</general><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>L6V</scope><scope>M7S</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>GOX</scope></search><sort><creationdate>20220528</creationdate><title>An Arduino-based, low-cost imaging incubator for extended live cell imaging</title><author>Rossi, Vincent M ; Davidson, Katherine C ; Moore, Lauren E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a529-6541a116b30a037c57ff0a79e14154d18fd74acd8fffe8212ec5a5d16bdef4423</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>Imaging</topic><topic>Low cost</topic><topic>Perturbation</topic><topic>Physics - Instrumentation and Detectors</topic><topic>Physics - Medical Physics</topic><toplevel>online_resources</toplevel><creatorcontrib>Rossi, Vincent M</creatorcontrib><creatorcontrib>Davidson, Katherine C</creatorcontrib><creatorcontrib>Moore, Lauren E</creatorcontrib><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Materials Science &amp; Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Engineering Collection</collection><collection>Engineering Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>arXiv.org</collection><jtitle>arXiv.org</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rossi, Vincent M</au><au>Davidson, Katherine C</au><au>Moore, Lauren E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An Arduino-based, low-cost imaging incubator for extended live cell imaging</atitle><jtitle>arXiv.org</jtitle><date>2022-05-28</date><risdate>2022</risdate><eissn>2331-8422</eissn><abstract>In order to image live cells for prolonged periods of time, an Arduino-based, low-cost imaging incubator was constructed. The imaging incubator keeps cells viable by controlling for temperature and CO2 in order to maintain physiological conditions for cells during imaging. All devices and parts employed in the build were typical maker-type components in order to minimize the cost of the imaging incubator. The imaging incubator allows for real-time imaging of live cells exposed to any desired perturbation or stimulus. As a proof of the system's functionality, cells are imaged over 24 hours while remaining viable in the imaging incubator.</abstract><cop>Ithaca</cop><pub>Cornell University Library, arXiv.org</pub><doi>10.48550/arxiv.2206.02542</doi><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier EISSN: 2331-8422
ispartof arXiv.org, 2022-05
issn 2331-8422
language eng
recordid cdi_arxiv_primary_2206_02542
source arXiv.org; Free E- Journals
subjects Imaging
Low cost
Perturbation
Physics - Instrumentation and Detectors
Physics - Medical Physics
title An Arduino-based, low-cost imaging incubator for extended live cell imaging
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-04T19%3A17%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_arxiv&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=An%20Arduino-based,%20low-cost%20imaging%20incubator%20for%20extended%20live%20cell%20imaging&rft.jtitle=arXiv.org&rft.au=Rossi,%20Vincent%20M&rft.date=2022-05-28&rft.eissn=2331-8422&rft_id=info:doi/10.48550/arxiv.2206.02542&rft_dat=%3Cproquest_arxiv%3E2673710269%3C/proquest_arxiv%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2673710269&rft_id=info:pmid/&rfr_iscdi=true