Probing the pH microenvironment of mesenchymal stromal cell cultures on additive-manufactured scaffolds
Despite numerous advances in the field of tissue engineering and regenerative medicine, monitoring the formation of tissue regeneration and its metabolic variations during culture is still a challenge and mostly limited to bulk volumetric assays. Here, a simple method of adding capsules based optica...
Gespeichert in:
| Veröffentlicht in: | arXiv.org 2021-02 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | arXiv.org |
| container_volume | |
| creator | Ivan Lorenzo Moldero Chandra, Anil Cavo, Marta Mota, Carlos Kapsokalyvas, Dimitrios Gigli, Giuseppe Moroni, Lorenzo del Mercato, Loretta L |
| description | Despite numerous advances in the field of tissue engineering and regenerative medicine, monitoring the formation of tissue regeneration and its metabolic variations during culture is still a challenge and mostly limited to bulk volumetric assays. Here, a simple method of adding capsules based optical sensors in cell seeded 3D scaffolds is presented and the potential of these sensors to monitor the pH changes in space and time during cell growth is demonstrated. It is shown that the pH decreased over time in the 3D scaffolds, with a more prominent decrease at the edges of the scaffolds. Moreover, the pH change is higher in 3D scaffolds compared to monolayered 2D cell cultures. The results suggest that this system, composed by capsules based optical sensors and 3D scaffolds with predefined geometry and pore architecture network, can be a suitable platform for monitoring pH variations during 3D cell growth and tissue formation. This is particularly relevant for the investigation of 3D cellular microenvironment alterations occurring both during physiological processes, such as tissue regeneration, and pathological processes, such as cancer evolution. |
| doi_str_mv | 10.48550/arxiv.2102.13168 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_arxiv</sourceid><recordid>TN_cdi_arxiv_primary_2102_13168</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2494718064</sourcerecordid><originalsourceid>FETCH-LOGICAL-a524-13b02c9221d58a8d5df1b424efb0dc6add18834baf2d1e524bcbc6b826ca85e83</originalsourceid><addsrcrecordid>eNotkEtrAjEUhUOhULH-gK4a6HpscpOMcVmkLxDahfshT43MJDaZkfrvO2o39yzuuedcPoQeKJlzKQR5Vvk3HOdACcwpo7W8QRNgjFaSA9yhWSl7QgjUCxCCTdD2Oycd4hb3O4cPH7gLJicXjyGn2LnY4-Rx54qLZnfqVItLn9NZjWvHMbT9kF3BKWJlbejD0VWdioNX5rywuBjlfWptuUe3XrXFzf51ijZvr5vVR7X-ev9cvawrJYBXlGkCZglArZBKWmE91Ry485pYU48dVErGtfJgqRsvtNGm1hJqo6Rwkk3R4zX2QqE55NCpfGrONJoLjdHxdHUccvoZXOmbfRpyHH9qgC_5gkpSc_YHpRhlIQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2494718064</pqid></control><display><type>article</type><title>Probing the pH microenvironment of mesenchymal stromal cell cultures on additive-manufactured scaffolds</title><source>Freely Accessible Journals</source><source>arXiv.org</source><creator>Ivan Lorenzo Moldero ; Chandra, Anil ; Cavo, Marta ; Mota, Carlos ; Kapsokalyvas, Dimitrios ; Gigli, Giuseppe ; Moroni, Lorenzo ; del Mercato, Loretta L</creator><creatorcontrib>Ivan Lorenzo Moldero ; Chandra, Anil ; Cavo, Marta ; Mota, Carlos ; Kapsokalyvas, Dimitrios ; Gigli, Giuseppe ; Moroni, Lorenzo ; del Mercato, Loretta L</creatorcontrib><description>Despite numerous advances in the field of tissue engineering and regenerative medicine, monitoring the formation of tissue regeneration and its metabolic variations during culture is still a challenge and mostly limited to bulk volumetric assays. Here, a simple method of adding capsules based optical sensors in cell seeded 3D scaffolds is presented and the potential of these sensors to monitor the pH changes in space and time during cell growth is demonstrated. It is shown that the pH decreased over time in the 3D scaffolds, with a more prominent decrease at the edges of the scaffolds. Moreover, the pH change is higher in 3D scaffolds compared to monolayered 2D cell cultures. The results suggest that this system, composed by capsules based optical sensors and 3D scaffolds with predefined geometry and pore architecture network, can be a suitable platform for monitoring pH variations during 3D cell growth and tissue formation. This is particularly relevant for the investigation of 3D cellular microenvironment alterations occurring both during physiological processes, such as tissue regeneration, and pathological processes, such as cancer evolution.</description><identifier>EISSN: 2331-8422</identifier><identifier>DOI: 10.48550/arxiv.2102.13168</identifier><language>eng</language><publisher>Ithaca: Cornell University Library, arXiv.org</publisher><subject>Cell culture ; Cell growth ; Monitoring ; Optical measuring instruments ; Physics - Biological Physics ; Physics - Medical Physics ; Quantitative Biology - Tissues and Organs ; Regeneration (physiology) ; Scaffolds ; Sensors ; Tissue engineering</subject><ispartof>arXiv.org, 2021-02</ispartof><rights>2021. This work is published under http://arxiv.org/licenses/nonexclusive-distrib/1.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>http://arxiv.org/licenses/nonexclusive-distrib/1.0</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>228,230,777,781,882,27906</link.rule.ids><backlink>$$Uhttps://doi.org/10.1002/smll.202002258$$DView published paper (Access to full text may be restricted)$$Hfree_for_read</backlink><backlink>$$Uhttps://doi.org/10.48550/arXiv.2102.13168$$DView paper in arXiv$$Hfree_for_read</backlink></links><search><creatorcontrib>Ivan Lorenzo Moldero</creatorcontrib><creatorcontrib>Chandra, Anil</creatorcontrib><creatorcontrib>Cavo, Marta</creatorcontrib><creatorcontrib>Mota, Carlos</creatorcontrib><creatorcontrib>Kapsokalyvas, Dimitrios</creatorcontrib><creatorcontrib>Gigli, Giuseppe</creatorcontrib><creatorcontrib>Moroni, Lorenzo</creatorcontrib><creatorcontrib>del Mercato, Loretta L</creatorcontrib><title>Probing the pH microenvironment of mesenchymal stromal cell cultures on additive-manufactured scaffolds</title><title>arXiv.org</title><description>Despite numerous advances in the field of tissue engineering and regenerative medicine, monitoring the formation of tissue regeneration and its metabolic variations during culture is still a challenge and mostly limited to bulk volumetric assays. Here, a simple method of adding capsules based optical sensors in cell seeded 3D scaffolds is presented and the potential of these sensors to monitor the pH changes in space and time during cell growth is demonstrated. It is shown that the pH decreased over time in the 3D scaffolds, with a more prominent decrease at the edges of the scaffolds. Moreover, the pH change is higher in 3D scaffolds compared to monolayered 2D cell cultures. The results suggest that this system, composed by capsules based optical sensors and 3D scaffolds with predefined geometry and pore architecture network, can be a suitable platform for monitoring pH variations during 3D cell growth and tissue formation. This is particularly relevant for the investigation of 3D cellular microenvironment alterations occurring both during physiological processes, such as tissue regeneration, and pathological processes, such as cancer evolution.</description><subject>Cell culture</subject><subject>Cell growth</subject><subject>Monitoring</subject><subject>Optical measuring instruments</subject><subject>Physics - Biological Physics</subject><subject>Physics - Medical Physics</subject><subject>Quantitative Biology - Tissues and Organs</subject><subject>Regeneration (physiology)</subject><subject>Scaffolds</subject><subject>Sensors</subject><subject>Tissue engineering</subject><issn>2331-8422</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GOX</sourceid><recordid>eNotkEtrAjEUhUOhULH-gK4a6HpscpOMcVmkLxDahfshT43MJDaZkfrvO2o39yzuuedcPoQeKJlzKQR5Vvk3HOdACcwpo7W8QRNgjFaSA9yhWSl7QgjUCxCCTdD2Oycd4hb3O4cPH7gLJicXjyGn2LnY4-Rx54qLZnfqVItLn9NZjWvHMbT9kF3BKWJlbejD0VWdioNX5rywuBjlfWptuUe3XrXFzf51ijZvr5vVR7X-ev9cvawrJYBXlGkCZglArZBKWmE91Ry485pYU48dVErGtfJgqRsvtNGm1hJqo6Rwkk3R4zX2QqE55NCpfGrONJoLjdHxdHUccvoZXOmbfRpyHH9qgC_5gkpSc_YHpRhlIQ</recordid><startdate>20210225</startdate><enddate>20210225</enddate><creator>Ivan Lorenzo Moldero</creator><creator>Chandra, Anil</creator><creator>Cavo, Marta</creator><creator>Mota, Carlos</creator><creator>Kapsokalyvas, Dimitrios</creator><creator>Gigli, Giuseppe</creator><creator>Moroni, Lorenzo</creator><creator>del Mercato, Loretta L</creator><general>Cornell University Library, arXiv.org</general><scope>8FE</scope><scope>8FG</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>HCIFZ</scope><scope>L6V</scope><scope>M7S</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>ALC</scope><scope>GOX</scope></search><sort><creationdate>20210225</creationdate><title>Probing the pH microenvironment of mesenchymal stromal cell cultures on additive-manufactured scaffolds</title><author>Ivan Lorenzo Moldero ; Chandra, Anil ; Cavo, Marta ; Mota, Carlos ; Kapsokalyvas, Dimitrios ; Gigli, Giuseppe ; Moroni, Lorenzo ; del Mercato, Loretta L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a524-13b02c9221d58a8d5df1b424efb0dc6add18834baf2d1e524bcbc6b826ca85e83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Cell culture</topic><topic>Cell growth</topic><topic>Monitoring</topic><topic>Optical measuring instruments</topic><topic>Physics - Biological Physics</topic><topic>Physics - Medical Physics</topic><topic>Quantitative Biology - Tissues and Organs</topic><topic>Regeneration (physiology)</topic><topic>Scaffolds</topic><topic>Sensors</topic><topic>Tissue engineering</topic><toplevel>online_resources</toplevel><creatorcontrib>Ivan Lorenzo Moldero</creatorcontrib><creatorcontrib>Chandra, Anil</creatorcontrib><creatorcontrib>Cavo, Marta</creatorcontrib><creatorcontrib>Mota, Carlos</creatorcontrib><creatorcontrib>Kapsokalyvas, Dimitrios</creatorcontrib><creatorcontrib>Gigli, Giuseppe</creatorcontrib><creatorcontrib>Moroni, Lorenzo</creatorcontrib><creatorcontrib>del Mercato, Loretta L</creatorcontrib><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Engineering Collection</collection><collection>Engineering Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>arXiv Quantitative Biology</collection><collection>arXiv.org</collection><jtitle>arXiv.org</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ivan Lorenzo Moldero</au><au>Chandra, Anil</au><au>Cavo, Marta</au><au>Mota, Carlos</au><au>Kapsokalyvas, Dimitrios</au><au>Gigli, Giuseppe</au><au>Moroni, Lorenzo</au><au>del Mercato, Loretta L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Probing the pH microenvironment of mesenchymal stromal cell cultures on additive-manufactured scaffolds</atitle><jtitle>arXiv.org</jtitle><date>2021-02-25</date><risdate>2021</risdate><eissn>2331-8422</eissn><abstract>Despite numerous advances in the field of tissue engineering and regenerative medicine, monitoring the formation of tissue regeneration and its metabolic variations during culture is still a challenge and mostly limited to bulk volumetric assays. Here, a simple method of adding capsules based optical sensors in cell seeded 3D scaffolds is presented and the potential of these sensors to monitor the pH changes in space and time during cell growth is demonstrated. It is shown that the pH decreased over time in the 3D scaffolds, with a more prominent decrease at the edges of the scaffolds. Moreover, the pH change is higher in 3D scaffolds compared to monolayered 2D cell cultures. The results suggest that this system, composed by capsules based optical sensors and 3D scaffolds with predefined geometry and pore architecture network, can be a suitable platform for monitoring pH variations during 3D cell growth and tissue formation. This is particularly relevant for the investigation of 3D cellular microenvironment alterations occurring both during physiological processes, such as tissue regeneration, and pathological processes, such as cancer evolution.</abstract><cop>Ithaca</cop><pub>Cornell University Library, arXiv.org</pub><doi>10.48550/arxiv.2102.13168</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | EISSN: 2331-8422 |
| ispartof | arXiv.org, 2021-02 |
| issn | 2331-8422 |
| language | eng |
| recordid | cdi_arxiv_primary_2102_13168 |
| source | Freely Accessible Journals; arXiv.org |
| subjects | Cell culture Cell growth Monitoring Optical measuring instruments Physics - Biological Physics Physics - Medical Physics Quantitative Biology - Tissues and Organs Regeneration (physiology) Scaffolds Sensors Tissue engineering |
| title | Probing the pH microenvironment of mesenchymal stromal cell cultures on additive-manufactured scaffolds |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-18T11%3A17%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_arxiv&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Probing%20the%20pH%20microenvironment%20of%20mesenchymal%20stromal%20cell%20cultures%20on%20additive-manufactured%20scaffolds&rft.jtitle=arXiv.org&rft.au=Ivan%20Lorenzo%20Moldero&rft.date=2021-02-25&rft.eissn=2331-8422&rft_id=info:doi/10.48550/arxiv.2102.13168&rft_dat=%3Cproquest_arxiv%3E2494718064%3C/proquest_arxiv%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2494718064&rft_id=info:pmid/&rfr_iscdi=true |