Immunological and Molecular Detection of Digenetic Infections in Different Species of Egyptian Freshwater Snails
Due to the possibility of utilizing different snails in the combat of Schistosoma in Egypt; it is important to study the role it may play in transmitting other trematodes of medical and veterinary importance. Taking this background into consideration, polymerase chain reaction (PCR) assay was design...
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Veröffentlicht in: | Journal of the Egyptian Society of Parasitology 2013-04, Vol.43 (1), p.167-182 |
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container_title | Journal of the Egyptian Society of Parasitology |
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creator | Saad , Abdel-Hakim Hassan , Hany M Varjabedian , Kohar G Abdel-Gaber , Rewaida A Abdel-Halim , Noha T |
description | Due to the possibility of utilizing different snails in the combat of Schistosoma in
Egypt; it is important to study the role it may play in transmitting other trematodes
of medical and veterinary importance. Taking this background into consideration,
polymerase chain reaction (PCR) assay was designed to identify trematode species
at larval stages in intermediate hosts (cercariae in snails) using a combination of
standard and molecular methods.
This PCR assay was also applied to naturally infected molluscan in order to assess
the use of the procedure for detection. The importance of the present study
was to demonstrate the epidemiological situation and application in control. |
doi_str_mv | 10.12816/0006375 |
format | Article |
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Egypt; it is important to study the role it may play in transmitting other trematodes
of medical and veterinary importance. Taking this background into consideration,
polymerase chain reaction (PCR) assay was designed to identify trematode species
at larval stages in intermediate hosts (cercariae in snails) using a combination of
standard and molecular methods.
This PCR assay was also applied to naturally infected molluscan in order to assess
the use of the procedure for detection. The importance of the present study
was to demonstrate the epidemiological situation and application in control.</description><identifier>ISSN: 1110-0583</identifier><identifier>EISSN: 2090-2549</identifier><identifier>DOI: 10.12816/0006375</identifier><identifier>PMID: 23697024</identifier><language>eng</language><publisher>Cairo - Egypt: The Egyptian Society of Parasitology</publisher><subject>Animals ; Biomphalaria ; EGYPT ; Fresh Water ; FRESHWATER ; IMMUNITY ; INFECTION ; NUCLEIC ACIDS ; PARASITES ; Polymerase Chain Reaction - methods ; Schistosoma - isolation & purification ; Sensitivity and Specificity ; SNAILS ; Snails - immunology ; Snails - parasitology ; Species Specificity ; الحلزون ; الحموض النووية ; الطفيليات ; العدوى ; المناعة ; المياه العذبة ; مصر</subject><ispartof>Journal of the Egyptian Society of Parasitology, 2013-04, Vol.43 (1), p.167-182</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttps://static.almanhal.com/covers/titl/53506/cover-lg.jpg</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23697024$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Saad , Abdel-Hakim</creatorcontrib><creatorcontrib>Hassan , Hany M</creatorcontrib><creatorcontrib>Varjabedian , Kohar G</creatorcontrib><creatorcontrib>Abdel-Gaber , Rewaida A</creatorcontrib><creatorcontrib>Abdel-Halim , Noha T</creatorcontrib><title>Immunological and Molecular Detection of Digenetic Infections in Different Species of Egyptian Freshwater Snails</title><title>Journal of the Egyptian Society of Parasitology</title><addtitle>J Egypt Soc Parasitol</addtitle><description>Due to the possibility of utilizing different snails in the combat of Schistosoma in
Egypt; it is important to study the role it may play in transmitting other trematodes
of medical and veterinary importance. Taking this background into consideration,
polymerase chain reaction (PCR) assay was designed to identify trematode species
at larval stages in intermediate hosts (cercariae in snails) using a combination of
standard and molecular methods.
This PCR assay was also applied to naturally infected molluscan in order to assess
the use of the procedure for detection. The importance of the present study
was to demonstrate the epidemiological situation and application in control.</description><subject>Animals</subject><subject>Biomphalaria</subject><subject>EGYPT</subject><subject>Fresh Water</subject><subject>FRESHWATER</subject><subject>IMMUNITY</subject><subject>INFECTION</subject><subject>NUCLEIC ACIDS</subject><subject>PARASITES</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Schistosoma - isolation & purification</subject><subject>Sensitivity and Specificity</subject><subject>SNAILS</subject><subject>Snails - immunology</subject><subject>Snails - parasitology</subject><subject>Species Specificity</subject><subject>الحلزون</subject><subject>الحموض النووية</subject><subject>الطفيليات</subject><subject>العدوى</subject><subject>المناعة</subject><subject>المياه العذبة</subject><subject>مصر</subject><issn>1110-0583</issn><issn>2090-2549</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpF0EFr2zAUB3BRVpaQFvYFOnTcxatkSZZ8HG2zBTp2aHs2L_JToiHLnmRT-u3nNml6eAj--vEe_An5wtl3XhpeXTPGKqHVGVmWrGZFqWT9iSw556xgyogFucz574x4JeQ8n8miFFWtWSmXZNh03RT70O-8hUAhtvR3H9BOARK9xRHt6PtIe0dv_Q4jjt7STXSHOFMf59w5TBhH-jCg9Zhf8d3uZRg9RLpOmPfPMGKiDxF8yBfk3EHIeHl8V-Rpffd486u4__Nzc_PjvoCSibFwtWHATVs5q7WptOC8MuiErEoQqFuObsthi7WVW7COWdSASjnNjGmFFGJFvh32Dqn_N2Eem85niyFAxH7KDRdKSa2kMB_Upj7nhK4Zku8gvTScNW8VN8eKZ_r1uHXadtie4HuhM7g6AJxzdHASQmqm2cc_hA7iHsIJKKHmG_8B87mKQg</recordid><startdate>201304</startdate><enddate>201304</enddate><creator>Saad , Abdel-Hakim</creator><creator>Hassan , Hany M</creator><creator>Varjabedian , Kohar G</creator><creator>Abdel-Gaber , Rewaida A</creator><creator>Abdel-Halim , Noha T</creator><general>The Egyptian Society of Parasitology</general><scope>~6Z</scope><scope>ADJCN</scope><scope>AHFXO</scope><scope>AHHHR</scope><scope>AHQOB</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201304</creationdate><title>Immunological and Molecular Detection of Digenetic Infections in Different Species of Egyptian Freshwater Snails</title><author>Saad , Abdel-Hakim ; 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Egypt; it is important to study the role it may play in transmitting other trematodes
of medical and veterinary importance. Taking this background into consideration,
polymerase chain reaction (PCR) assay was designed to identify trematode species
at larval stages in intermediate hosts (cercariae in snails) using a combination of
standard and molecular methods.
This PCR assay was also applied to naturally infected molluscan in order to assess
the use of the procedure for detection. The importance of the present study
was to demonstrate the epidemiological situation and application in control.</abstract><cop>Cairo - Egypt</cop><pub>The Egyptian Society of Parasitology</pub><pmid>23697024</pmid><doi>10.12816/0006375</doi><tpages>16</tpages></addata></record> |
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language | eng |
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source | MEDLINE; Alma/SFX Local Collection |
subjects | Animals Biomphalaria EGYPT Fresh Water FRESHWATER IMMUNITY INFECTION NUCLEIC ACIDS PARASITES Polymerase Chain Reaction - methods Schistosoma - isolation & purification Sensitivity and Specificity SNAILS Snails - immunology Snails - parasitology Species Specificity الحلزون الحموض النووية الطفيليات العدوى المناعة المياه العذبة مصر |
title | Immunological and Molecular Detection of Digenetic Infections in Different Species of Egyptian Freshwater Snails |
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