Effects of Mutations of the Active Site Arginine Residues in 4-Oxalocrotonate Tautomerase on the pK a Values of Active Site Residues and on the pH Dependence of Catalysis

The unusually low pK a value of the general base catalyst Pro-1 (pK a = 6.4) in 4-oxalocrotonate tautomerase (4-OT) has been ascribed to both a low dielectric constant at the active site and the proximity of the cationic residues Arg-11 and Arg-39 [Stivers, J. T., Abeygunawardana, C., Mildvan, A. S....

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Biochemistry (Easton) 1999-09, Vol.38 (38), p.12358-12366
Hauptverfasser:	Czerwinski, Robert M, Harris, Thomas K, Johnson, William H, Legler, Patricia M, Stivers, James T, Mildvan, Albert S, Whitman, Christian P
Format:	Artikel
Sprache:	eng
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!