A Bifunctional Polyphosphate Kinase Driving the Regeneration of Nucleoside Triphosphate and Reconstituted Cell-Free Protein Synthesis

Reconstituted cell-free protein synthesis systems (e.g., the PURE system) allow the expression of toxic proteins, hetero-oligomeric protein subunits, and proteins with noncanonical amino acids with high levels of homogeneity. In these systems, an artificial ATP/GTP regeneration system is required to...

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Veröffentlicht in:	ACS synthetic biology 2020-01, Vol.9 (1), p.36-42
Hauptverfasser:	Wang, Po-Hsiang, Fujishima, Kosuke, Berhanu, Samuel, Kuruma, Yutetsu, Jia, Tony Z, Khusnutdinova, Anna N, Yakunin, Alexander F, McGlynn, Shawn E
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Triphosphate - metabolism Amino Acyl-tRNA Synthetases - metabolism Animals Cell-Free System - metabolism Cytophaga - enzymology Fireflies - enzymology Green Fluorescent Proteins - metabolism Guanosine Triphosphate - metabolism Luciferases, Firefly - metabolism Phosphorylation Phosphotransferases (Phosphate Group Acceptor) - metabolism Polyphosphates - metabolism Protein Biosynthesis RNA, Messenger - metabolism RNA, Transfer, Amino Acid-Specific - metabolism
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