Cellular Delivery of Plasmid DNA into Wheat Microspores Using Rosette Nanotubes
Plant genetic engineering offers promising solutions to the increasing demand for efficient, sustainable, and high-yielding crop production as well as changing environmental conditions. The main challenge for gene delivery in plants is the presence of a cell wall that limits the transportation of ge...
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| Veröffentlicht in: | ACS omega 2020-09, Vol.5 (38), p.24422-24433 |
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| creator | Cho, Jae-Young Bhowmik, Pankaj Polowick, Patricia L Dodard, Sabine G El-Bakkari, Mounir Nowak, Goska Fenniri, Hicham Hemraz, Usha D |
| description | Plant genetic engineering offers promising solutions to the increasing demand for efficient, sustainable, and high-yielding crop production as well as changing environmental conditions. The main challenge for gene delivery in plants is the presence of a cell wall that limits the transportation of genes within the cells. Microspores are plant cells that are, under the right conditions, capable of generating embryos, leading to the formation of haploid plants. Here, we designed cationic and fluorescent rosette nanotubes (RNTs) that penetrate the cell walls of viable wheat microspores under mild conditions and in the absence of an external force. These nanomaterials can capture plasmid DNA to form RNT–DNA complexes and transport their DNA cargo into live microspores. The nanomaterials and the complexes formed were nontoxic to the microspores. |
| doi_str_mv | 10.1021/acsomega.0c02830 |
| format | Article |
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| subjects | Chemistry Chemistry, Multidisciplinary Physical Sciences Science & Technology |
| title | Cellular Delivery of Plasmid DNA into Wheat Microspores Using Rosette Nanotubes |
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