MoS2 Nanosheet-Doped Peptide-Imprinted Polymer-Coated Electrodes for Electrochemical Determination of CRISPR/dCas9-Activated Protein Expression

MoS2 Nanosheet-Doped Peptide-Imprinted Polymer-Coated Electrodes for Electrochemical Determination of CRISPR/dCas9-Activated Protein Expression

Matrix metalloproteinases (MMPs) are involved in the breakdown of the extracellular matrix (ECM) in disease processes; thus, measurement of MMPs is of potential clinical importance. In this work, a sensitive polymer-coated electrode for sensing MMP-1 was constructed using molecular imprinting of MMP...

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Veröffentlicht in:ACS applied nano materials 2023-10, Vol.6 (19), p.17369-17375
Hauptverfasser: Lee, Mei-Hwa, Lin, Cheng-Chih, Kutner, Wlodzimierz, Thomas, James L., Lin, Chu-Yun, Iskierko, Zofia, Lin, Chien-Yu, Sharma, Piyush S., Lin, Hung-Yin
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container_end_page 17375
container_issue 19
container_start_page 17369
container_title ACS applied nano materials
container_volume 6
creator Lee, Mei-Hwa
Lin, Cheng-Chih
Kutner, Wlodzimierz
Thomas, James L.
Lin, Chu-Yun
Iskierko, Zofia
Lin, Chien-Yu
Sharma, Piyush S.
Lin, Hung-Yin
description Matrix metalloproteinases (MMPs) are involved in the breakdown of the extracellular matrix (ECM) in disease processes; thus, measurement of MMPs is of potential clinical importance. In this work, a sensitive polymer-coated electrode for sensing MMP-1 was constructed using molecular imprinting of MMP-1 peptides. Molybdenum disulfide (MoS2) nanosheets were incorporated into peptide-imprinted electropolymerized poly­(triphenylamine rhodanine-3-acetic acid-co-3,4-ethoxylene dioxythiophene)­s, poly­(TPARA-co-EDOT)­s. Doping peptide-imprinted polymer-based electrochemical sensors with MoS2 nanosheets gives significant enhancement of the response (compared to that without MoS2 doping); this work also demonstrated their useful application in measuring the expression of MMP-1 by a CRISPR/dCas9a system.
doi_str_mv 10.1021/acsanm.3c04130
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