Fast and Ultrasensitive Visual Detection of Exosomes in Body Fluids for Point-of-Care Disease Diagnosis
Fast detection of low-concentration exosomes in body fluids is of great significance in understanding the pathogenesis and disease diagnosis but is quite a challenging work due to the complex matrix, tedious pretreatment, and relatively poor sensitivity without the aid of instruments. In this work,...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2021-07, Vol.93 (29), p.10372-10377 |
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| container_title | Analytical chemistry (Washington) |
| container_volume | 93 |
| creator | Chen, Shan Jiang, Tao Lin, Hao Chen, Junyan Yang, Shuangli Wang, Pengcheng Gan, Xinqiang Wang, Yali Xu, Bin Sun, Junjie Yin, Congcong Huang, Zongxiong Fang, Yimin |
| description | Fast detection of low-concentration exosomes in body fluids is of great significance in understanding the pathogenesis and disease diagnosis but is quite a challenging work due to the complex matrix, tedious pretreatment, and relatively poor sensitivity without the aid of instruments. In this work, by simply using a filter membrane to enrich the exosomes at low concentrations and the use of CuS nanoparticles as labels, we were able to detect exosomes at concentrations as low as 2 × 103 particles/μL in a complex matrix by the naked eye. Due to its high sensitivity, specificity, and simplicity, it can be used for the diagnosis of direct prostate cancer via a 5 mL urine sample within 2 h without the use of any instrument. This method can also be applicable for the detection of other biological nanoparticles, such as viruses, at low concentrations in a complex matrix, offering a promising candidate for point-of-care disease diagnosis with low cost. |
| doi_str_mv | 10.1021/acs.analchem.1c02136 |
| format | Article |
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In this work, by simply using a filter membrane to enrich the exosomes at low concentrations and the use of CuS nanoparticles as labels, we were able to detect exosomes at concentrations as low as 2 × 103 particles/μL in a complex matrix by the naked eye. Due to its high sensitivity, specificity, and simplicity, it can be used for the diagnosis of direct prostate cancer via a 5 mL urine sample within 2 h without the use of any instrument. This method can also be applicable for the detection of other biological nanoparticles, such as viruses, at low concentrations in a complex matrix, offering a promising candidate for point-of-care disease diagnosis with low cost.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/acs.analchem.1c02136</identifier><language>eng</language><publisher>Washington: American Chemical Society</publisher><subject>Body fluids ; Chemistry ; Copper sulfides ; Diagnosis ; Exosomes ; Low concentrations ; Medical diagnosis ; Nanoparticles ; Pathogenesis ; Prostate cancer ; Sensitivity ; Viruses</subject><ispartof>Analytical chemistry (Washington), 2021-07, Vol.93 (29), p.10372-10377</ispartof><rights>2021 American Chemical Society</rights><rights>Copyright American Chemical Society Jul 27, 2021</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a353t-1a370f78ab78e3c469364c8c93e0433d742c96adb11725bdafa661c13e65eee23</citedby><cites>FETCH-LOGICAL-a353t-1a370f78ab78e3c469364c8c93e0433d742c96adb11725bdafa661c13e65eee23</cites><orcidid>0000-0002-2735-6782</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.analchem.1c02136$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.analchem.1c02136$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>315,781,785,2766,27078,27926,27927,56740,56790</link.rule.ids></links><search><creatorcontrib>Chen, Shan</creatorcontrib><creatorcontrib>Jiang, Tao</creatorcontrib><creatorcontrib>Lin, Hao</creatorcontrib><creatorcontrib>Chen, Junyan</creatorcontrib><creatorcontrib>Yang, Shuangli</creatorcontrib><creatorcontrib>Wang, Pengcheng</creatorcontrib><creatorcontrib>Gan, Xinqiang</creatorcontrib><creatorcontrib>Wang, Yali</creatorcontrib><creatorcontrib>Xu, Bin</creatorcontrib><creatorcontrib>Sun, Junjie</creatorcontrib><creatorcontrib>Yin, Congcong</creatorcontrib><creatorcontrib>Huang, Zongxiong</creatorcontrib><creatorcontrib>Fang, Yimin</creatorcontrib><title>Fast and Ultrasensitive Visual Detection of Exosomes in Body Fluids for Point-of-Care Disease Diagnosis</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>Fast detection of low-concentration exosomes in body fluids is of great significance in understanding the pathogenesis and disease diagnosis but is quite a challenging work due to the complex matrix, tedious pretreatment, and relatively poor sensitivity without the aid of instruments. In this work, by simply using a filter membrane to enrich the exosomes at low concentrations and the use of CuS nanoparticles as labels, we were able to detect exosomes at concentrations as low as 2 × 103 particles/μL in a complex matrix by the naked eye. Due to its high sensitivity, specificity, and simplicity, it can be used for the diagnosis of direct prostate cancer via a 5 mL urine sample within 2 h without the use of any instrument. This method can also be applicable for the detection of other biological nanoparticles, such as viruses, at low concentrations in a complex matrix, offering a promising candidate for point-of-care disease diagnosis with low cost.</description><subject>Body fluids</subject><subject>Chemistry</subject><subject>Copper sulfides</subject><subject>Diagnosis</subject><subject>Exosomes</subject><subject>Low concentrations</subject><subject>Medical diagnosis</subject><subject>Nanoparticles</subject><subject>Pathogenesis</subject><subject>Prostate cancer</subject><subject>Sensitivity</subject><subject>Viruses</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9kDFP5DAQhS10SOwB_4DCEs01WcZ24iQlLCwgIUEBtNGsMwGjbAye5HT8ewwLFBRXPWnme6_4hDhQMFeg1RE6nuOAvXuk9Vy5dDJ2S8xUoSGzVaV_iRkAmEyXADviN_MTgFKg7Ew8LJFHiUMr7_oxItPAfvR_Sd57nrCXpzSSG30YZOjk2b_AYU0s_SBPQvsql_3kW5ZdiPIm-GHMQpctMJI89UxpLCU-DIE974ntDnum_c_cFXfLs9vFRXZ1fX65OL7K0BRmzBSaErqywlVZkXG5rY3NXeVqQ5Ab05a5drXFdqVUqYtVix1aq5wyZAsi0mZX_NnsPsfwMhGPzdqzo77HgcLEjS4KpaEqa0jo4Q_0KUwxafyg0nxtbZ6ofEO5GJgjdc1z9GuMr42C5t1-k-w3X_abT_upBpva-_d797-VN-nti2M</recordid><startdate>20210727</startdate><enddate>20210727</enddate><creator>Chen, Shan</creator><creator>Jiang, Tao</creator><creator>Lin, Hao</creator><creator>Chen, Junyan</creator><creator>Yang, Shuangli</creator><creator>Wang, Pengcheng</creator><creator>Gan, Xinqiang</creator><creator>Wang, Yali</creator><creator>Xu, Bin</creator><creator>Sun, Junjie</creator><creator>Yin, Congcong</creator><creator>Huang, Zongxiong</creator><creator>Fang, Yimin</creator><general>American Chemical Society</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-2735-6782</orcidid></search><sort><creationdate>20210727</creationdate><title>Fast and Ultrasensitive Visual Detection of Exosomes in Body Fluids for Point-of-Care Disease Diagnosis</title><author>Chen, Shan ; Jiang, Tao ; Lin, Hao ; Chen, Junyan ; Yang, Shuangli ; Wang, Pengcheng ; Gan, Xinqiang ; Wang, Yali ; Xu, Bin ; Sun, Junjie ; Yin, Congcong ; Huang, Zongxiong ; Fang, Yimin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a353t-1a370f78ab78e3c469364c8c93e0433d742c96adb11725bdafa661c13e65eee23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Body fluids</topic><topic>Chemistry</topic><topic>Copper sulfides</topic><topic>Diagnosis</topic><topic>Exosomes</topic><topic>Low concentrations</topic><topic>Medical diagnosis</topic><topic>Nanoparticles</topic><topic>Pathogenesis</topic><topic>Prostate cancer</topic><topic>Sensitivity</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chen, Shan</creatorcontrib><creatorcontrib>Jiang, Tao</creatorcontrib><creatorcontrib>Lin, Hao</creatorcontrib><creatorcontrib>Chen, Junyan</creatorcontrib><creatorcontrib>Yang, Shuangli</creatorcontrib><creatorcontrib>Wang, Pengcheng</creatorcontrib><creatorcontrib>Gan, Xinqiang</creatorcontrib><creatorcontrib>Wang, Yali</creatorcontrib><creatorcontrib>Xu, Bin</creatorcontrib><creatorcontrib>Sun, Junjie</creatorcontrib><creatorcontrib>Yin, Congcong</creatorcontrib><creatorcontrib>Huang, Zongxiong</creatorcontrib><creatorcontrib>Fang, Yimin</creatorcontrib><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, Shan</au><au>Jiang, Tao</au><au>Lin, Hao</au><au>Chen, Junyan</au><au>Yang, Shuangli</au><au>Wang, Pengcheng</au><au>Gan, Xinqiang</au><au>Wang, Yali</au><au>Xu, Bin</au><au>Sun, Junjie</au><au>Yin, Congcong</au><au>Huang, Zongxiong</au><au>Fang, Yimin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fast and Ultrasensitive Visual Detection of Exosomes in Body Fluids for Point-of-Care Disease Diagnosis</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. 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| source | ACS Journals: American Chemical Society Web Editions |
| subjects | Body fluids Chemistry Copper sulfides Diagnosis Exosomes Low concentrations Medical diagnosis Nanoparticles Pathogenesis Prostate cancer Sensitivity Viruses |
| title | Fast and Ultrasensitive Visual Detection of Exosomes in Body Fluids for Point-of-Care Disease Diagnosis |
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