Evaluating Hedgehog Acyltransferase Activity and Inhibition Using the Acylation-coupled Lipophilic Induction of Polarization (Acyl-cLIP) Assay von Andrei, Sebastian A., Tate, Edward W., Lanyon-Hogg, Thomas

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An In Situ Fluorescence Assay for Cholesterol Transporter Activity of the Patched von Cho, Wonhwa, Ralko, Arthur, Sharma, Ashutosh

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Generation and Analysis of Mosaic Spinal Cord Organoids Derived from Mouse Embryonic Stem Cells von Jägers, Carina, Roelink, Henk

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A Method for Orthotopic Transplantation of Lung Cancer in Mice von Wilson, Alexandra N., Chen, Baozhi, Liu, Xin, Kurie, Jonathan M., Kim, James

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The Immunofluorescence-Based Detection of Hedgehog Pathway Components in Primary Cilia of Cultured Cells von Yang, Shu, Zheng, Xiaoyan

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Cell-Based Assays for Smoothened Ubiquitination and Sumoylation von Han, Yuhong, Jiang, Jin

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Measuring and Manipulating Membrane Cholesterol for the Study of Hedgehog Signaling von Kinnebrew, Maia, Johnson, Kristen A., Radhakrishnan, Arun, Rohatgi, Rajat

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Ci/Gli Phosphorylation by the Fused/Ulk Family Kinases von Han, Yuhong, Jiang, Jin

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Imaging of the Ciliary Cholesterol Underlying the Sonic Hedgehog Signal Transduction von Miyamoto, Tatsuo, Hosoba, Kosuke, Akutsu, Silvia Natsuko, Matsuura, Shinya

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Characterization of Smoothened Phosphorylation and Activation von Jia, Jianhang, Han, Yuhong, Cho, Yong Suk, Jiang, Jin

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Biochemical Assays to Directly Assess Smoothened Activation by a Conformationally Sensitive Nanobody von Deshpande, Ishan, Manglik, Aashish

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Light-Induced Differentiation of Forebrain Organoids by NVOC-SAG von Misawa, Ryuji, Ikeuchi, Yoshiho

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NanoBRET and NanoBiT/BRET-Based Ligand Binding Assays Permit Quantitative Assessment of Small Molecule Ligand Binding to Smoothened von Kozielewicz, Paweł, Schulte, Gunnar

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Rapid, Direct SMOOTHENED Activity Assays in Live Cells Using cAMP-Based Conformational Sensors von Walker, Madison F., Myers, Benjamin R.

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Analysis of Protein Cholesterylation by Biorthogonal Labeling von Hu, Ao, Zhou, Meng, Song, Bao-Liang

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Measuring Smoothened (SMO)-Mediated Activation of the Gi Protein von Liu, Heng, Zhang, Cheng

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Expression, Purification, and Structure Determination of Human PTCH1–HH-N Complexes von Qi, Xiaofeng, Schmiege, Philip, Esparza, Leticia, Li, Xiaochun

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Studying Hedgehog Signaling During Mouse Neural Tube Development von Constable, Sandii, White, Kevin, Somatilaka, Bandarigoda Nipunika, Mukhopadhyay, Saikat

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Studies of SMOOTHENED Activation in Cell-Free and Reconstituted Systems von Nelson, Isaac B., Myers, Benjamin R.

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Analysis of Dispatched Protein Processing and Sonic Hedgehog Ligand Release von Cleverdon, Elizabeth, Stewart, Daniel P., Ogden, Stacey K.

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