Molecular Basis of Assembly and Activation of Complement Component C1 in Complex with Immunoglobulin G1 and Antigen von Wang, Guanbo, de Jong, Rob N., van den Bremer, Ewald T.J., Beurskens, Frank J., Labrijn, Aran F., Ugurlar, Deniz, Gros, Piet, Schuurman, Janine, Parren, Paul W.H.I., Heck, Albert J.R.

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Modulation of antibody galactosylation through feeding of uridine, manganese chloride, and galactose von Gramer, Michael J., Eckblad, Jackie J., Donahue, Ruth, Brown, Joseph, Shultz, Carrie, Vickerman, Kent, Priem, Patrick, van den Bremer, Ewald T.J., Gerritsen, Jolanda, van Berkel, Patrick H.C.

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Enhancing Accuracy in Molecular Weight Determination of Highly Heterogeneously Glycosylated Proteins by Native Tandem Mass Spectrometry von Wang, Guanbo, de Jong, Rob N, van den Bremer, Ewald T. J, Parren, Paul W. H. I, Heck, Albert J. R

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Controlled Fab-arm exchange for the generation of stable bispecific IgG1 von Labrijn, Aran F, Meesters, Joyce I, Priem, Patrick, de Jong, Rob N, van den Bremer, Ewald T J, van Kampen, Muriel D, Gerritsen, Arnout F, Schuurman, Janine, Parren, Paul W H I

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Efficient generation of stable bispecific IgG1 by controlled Fab-arm exchange von Labrijn, Aran F, Meesters, Joyce I, de Goeij, Bart E C G, van den Bremer, Ewald T J, Neijssen, Joost, van Kampen, Muriel D, Strumane, Kristin, Verploegen, Sandra, Kundu, Amitava, Gramer, Michael J, van Berkel, Patrick H C, van de Winkel, Jan G J, Schuurman, Janine, Parren, Paul W H I

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Tandem Native Mass-Spectrometry on Antibody–Drug Conjugates and Submillion Da Antibody–Antigen Protein Assemblies on an Orbitrap EMR Equipped with a High-Mass Quadrupole Mass Selec... von Dyachenko, Andrey, Wang, Guanbo, Belov, Mike, Makarov, Alexander, de Jong, Rob N, van den Bremer, Ewald T. J, Parren, Paul W. H. I, Heck, Albert J. R

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Therapeutic IgG4 antibodies engage in Fab-arm exchange with endogenous human IgG4 in vivo von Parren, Paul W H I, Labrijn, Aran F, Buijsse, Antonio Ortiz, van den Bremer, Ewald T J, Verwilligen, Annemiek Y W, Bleeker, Wim K, Thorpe, Susan J, Killestein, Joep, Polman, Chris H, Aalberse, Rob C, Schuurman, Janine, van de Winkel, Jan G J

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Quantitative Analysis of the Interaction Strength and Dynamics of Human IgG4 Half Molecules by Native Mass Spectrometry von Rose, Rebecca J., Labrijn, Aran F., van den Bremer, Ewald T.J., Loverix, Stefan, Lasters, Ignace, van Berkel, Patrick H.C., van de Winkel, Jan G.J., Schuurman, Janine, Parren, Paul W.H.I., Heck, Albert J.R.

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Species-specific determinants in the IgG CH3 domain enable Fab-arm exchange by affecting the noncovalent CH3-CH3 interaction strength von Labrijn, Aran F, Rispens, Theo, Meesters, Joyce, Rose, Rebecca J, den Bleker, Tamara H, Loverix, Stefan, van den Bremer, Ewald T J, Neijssen, Joost, Vink, Tom, Lasters, Ignace, Aalberse, Rob C, Heck, Albert J R, van de Winkel, Jan G J, Schuurman, Janine, Parren, Paul W H I

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Human IgG is produced in a pro-form that requires clipping of C-terminal lysines for maximal complement activation von van den Bremer, Ewald TJ, Beurskens, Frank J, Voorhorst, Marleen, Engelberts, Patrick J, de Jong, Rob N, van der Boom, Burt G, Cook, Erika M, Lindorfer, Margaret A, Taylor, Ronald P, van Berkel, Patrick HC, Parren, Paul WHI

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Cysteine-SILAC Mass Spectrometry Enabling the Identification and Quantitation of Scrambled Interchain Disulfide Bonds: Preservation of Native Heavy-Light Chain Pairing in Bispecifi... von van den Bremer, Ewald T. J, Labrijn, Aran F, van den Boogaard, Ramon, Priem, Patrick, Scheffler, Kai, Melis, Joost P. M, Schuurman, Janine, Parren, Paul W. H. I, de Jong, Rob N

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Production of stable bispecific IgG1 by controlled Fab-arm exchange: Scalability from bench to large-scale manufacturing by application of standard approaches von Gramer, Michael J, van den Bremer, Ewald TJ, van Kampen, Muriel D, Kundu, Amitava, Kopfmann, Peter, Etter, Eric, Stinehelfer, David, Long, Justin, Lannom, Tom, Noordergraaf, Esther H, Gerritsen, Jolanda, Labrijn, Aran F, Schuurman, Janine, van Berkel, Patrick HC, Parren, Paul WHI

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In-depth qualitative and quantitative analysis of composite glycosylation profiles and other micro-heterogeneity on intact monoclonal antibodies by high-resolution native mass spec... von Rosati, Sara, van den Bremer, Ewald TJ, Schuurman, Janine, Parren, Paul WHI, Kamerling, Johannis P, Heck, Albert JR

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Mutation of Y407 in the CH3 domain dramatically alters glycosylation and structure of human IgG von Rose, Rebecca J., van Berkel, Patrick H.C., van den Bremer, Ewald T.J., Labrijn, Aran F., Vink, Tom, Schuurman, Janine, Heck, Albert J.R., Parren, Paul W.H.I.

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Biophysical Characterization and Stability of Modified IgG1 Antibodies with Different Hexamerization Propensities von van Kampen, Muriel D., Kuipers-De Wilt, Leonie H.A.M., van Egmond, Mariëlle L., Reinders-Blankert, Petra, van den Bremer, Ewald T.J., Wang, Guanbo, Heck, Albert J.R., Parren, Paul W.H.I., Beurskens, Frank J., Schuurman, Janine, de Jong, Rob N.

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Ligand-Induced Changes in the Conformational Dynamics of a Bacterial Cytotoxic Endonuclease von van den Bremer, Ewald T. J, Keeble, Anthony H, Visser, Antonie J. W. G, van Hoek, Arie, Kleanthous, Colin, Heck, Albert J. R, Jiskoot, Wim

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Probing metal ion binding and conformational properties of the colicin E9 endonuclease by electrospray ionization time‐of‐flight mass spectrometry von van den Bremer, Ewald T.J., Jiskoot, Wim, James, Richard, Moore, Geoffrey R., Kleanthous, Colin, Heck, Albert J.R., Maier, Claudia S.

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Impact of IgG oligomerization state on the potency of complement-dependent cytotoxicity von Beurskens, Frank J, de Jong, Rob N, Wang, Guanbo, Voorhorst, Marleen, van den Bremer, Ewald T.J, Labrijn, Aran F, Schuurman, Janine, Heck, Albert J.R, Parren, Paul W.H.I

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Metal induced selectivity in phosphate ion binding in E9 DNase von van den Bremer, Ewald T J, Keeble, Anthony H, Kleanthous, Colin, Heck, Albert J R

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Biophysical characterization and stability of modified IgG1 antibodies with different hexamerization propensities von van Kampen, Muriel D, Kuipers-De Wilt, Leonie H A M, van Egmond, Mariëlle L, Reinders-Blankert, Petra, van den Bremer, Ewald T J, Wang, Guanbo, Heck, Albert J R, Parren, Paul W H I, Beurskens, Frank J, Schuurman, Janine, de Jong, Rob N

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