High-throughput nuclear delivery and rapid expression of DNA via mechanical and electrical cell-membrane disruption von Ding, Xiaoyun, Stewart, Martin P., Sharei, Armon, Weaver, James C., Langer, Robert S., Jensen, Klavs F.

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Live-cell protein labelling with nanometre precision by cell squeezing von Kollmannsperger, Alina, Sharei, Armon, Raulf, Anika, Heilemann, Mike, Langer, Robert, Jensen, Klavs F., Wieneke, Ralph, Tampé, Robert

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Microfluidics-Based Assessment of Cell Deformability von Adamo, Andrea, Sharei, Armon, Adamo, Luigi, Lee, ByungKun, Mao, Shirley, Jensen, Klavs F

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vector-free microfluidic platform for intracellular delivery von Sharei, Armon, Zoldan, Janet, Adamo, Andrea, Sim, Woo Young, Cho, Nahyun, Jackson, Emily, Mao, Shirley, Schneider, Sabine, Han, Min-Joon, Lytton-Jean, Abigail, Basto, Pamela A., Jhunjhunwala, Siddharth, Lee, Jungmin, Heller, Daniel A., Kang, Jeon Woong, Hartoularos, George C., Kim, Kwang-Soo, Anderson, Daniel G., Langer, Robert, Jensen, Klavs F.

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Microfluidic squeezing for intracellular antigen loading in polyclonal B-cells as cellular vaccines von Szeto, Gregory Lee, Van Egeren, Debra, Worku, Hermoon, Sharei, Armon, Alejandro, Brian, Park, Clara, Frew, Kirubel, Brefo, Mavis, Mao, Shirley, Heimann, Megan, Langer, Robert, Jensen, Klavs, Irvine, Darrell J

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Flow-Through Comb Electroporation Device for Delivery of Macromolecules von Adamo, Andrea, Arione, Alessandro, Sharei, Armon, Jensen, Klavs F

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Engineered RBCs Encapsulating Antigen Induce Multi-Modal Antigen-Specific Tolerance and Protect Against Type 1 Diabetes von Raposo, Colin J, Cserny, Judith D, Serena, Gloria, Chow, Jonathan N, Cho, Patricia, Liu, Hanyang, Kotler, David, Sharei, Armon, Bernstein, Howard, John, Shinu

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Ex vivo cytosolic delivery of functional macromolecules to immune cells von Sharei, Armon, Trifonova, Radiana, Jhunjhunwala, Siddharth, Hartoularos, George C, Eyerman, Alexandra T, Lytton-Jean, Abigail, Angin, Mathieu, Sharma, Siddhartha, Poceviciute, Roberta, Mao, Shirley, Heimann, Megan, Liu, Sophia, Talkar, Tanya, Khan, Omar F, Addo, Marylyn, von Andrian, Ulrich H, Anderson, Daniel G, Langer, Robert, Lieberman, Judy, Jensen, Klavs F

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Highly Efficient Patterning of Organic Single-Crystal Transistors from the Solution Phase von Mannsfeld, Stefan C. B., Sharei, Armon, Liu, Shuhong, Roberts, Mark E., McCulloch, Iain, Heeney, Martin, Bao, Zhenan

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Engineered red blood cells (activating antigen carriers) drive potent T cell responses and tumor regression in mice von Blagovic, Katarina, Smith, Carolyne K., Ramakrishnan, Amritha, Moore, Lindsay, Soto, David R., Thompson, Zachary, Stockmann, Adam P., Kruszelnicki, Sonia, Thakkar, Akshi, Murray, Jason, Torres, Sebastian, Wondimagegnhu, Bersabel, Yi, Roslyn, Dadgar, Maisam, Paracha, Abdul M., Page, Claire, Clear, Louise, Chaudhry, Omer A., Myint, Melissa, Bridgen, Devin T., Gilbert, Jonathan B., Seidl, Katherine J., Sharei, Armon, Loughhead, Scott, Bernstein, Howard, Yarar, Defne

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165 Generating enhanced tumor infiltrating lymphocytes through microfluidic cell squeezing von Bridgen, Devin, Bhattacharjee, Arindam, Thalhofer, Colin, Montler, Ryan, Weinberg, Andrew, Sharei, Armon, Gilbert, Jonathan

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Plasma membrane recovery kinetics of a microfluidic intracellular delivery platform von Sharei, Armon, Poceviciute, Roberta, Jackson, Emily L, Cho, Nahyun, Mao, Shirley, Hartoularos, George C, Jang, Derek Y, Jhunjhunwala, Siddharth, Eyerman, Alexandra, Schoettle, Taylor, Langer, Robert, Jensen, Klavs F

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165 Activating antigen carriers generated with microfluidics cell squeezing drive effective anti-tumor responses von Blagovic, Katarina, Ramakrishnan, Amritha, Sharei, Armon, Bernstein, Howard, Seidl, Katherine, Yarar, Defne

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156 RBC-derived, activating antigen carriers (SQZ AACs) prime potent T cell responses and drive tumor regression in vivo von Blagovic, Katarina, Smith, Carolyne, Moore, Lindsay, Ozay, Emrah Ilker, Sharei, Armon, Bernstein, Howard, Loughhead, Scott

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In vitro and ex vivo strategies for intracellular delivery von Stewart, Martin P., Sharei, Armon, Ding, Xiaoyun, Sahay, Gaurav, Langer, Robert, Jensen, Klavs F.

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211 SQZ™ eAPCs generated from PBMCs by delivery of multiple mRNAs encoding for antigens, costimulatory proteins, and engineered cytokines von Maloney, Michael, Ozay, Emrah Ilker, Merino, Amy, Silva, Andrea, Martin, Amber, Manja, Sanjana, Upadhyay, Madhav, Trumpfheller, Christine, Umana, Pablo, Sharei, Armon, Bernstein, Howard, Loughhead, Scott

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170 Microfluidics cell squeezing enables potent cellular vaccines in murine models through direct cytosolic loading and direct CD8 T cell priming von Ozay, Emrah, Booty, Matthew, Blagovic, Katarina, Soto, David, Pryor, Olivia, Stockmann, Adam, Subramanya, Disha, Volk, Kelly, Sharei, Armon, Bernstein, Howard, Yarar, Defne, Seidl, Katherine, Loughhead, Scott

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169 Microfluidics cell squeezing enables human PBMCs as drivers of antigen-specific CD8 T responses across broad range of antigens for diverse clinical applications von Maloney, Michael, Loughhead, Scott, Ramakrishnan, Amritha, Smith, Carolyne, Venkitaraman, Anita, Yee, Christian, Jacques, Miye, Yarar, Defne, Sharei, Armon, Bernstein, Howard, Hlavaty, Kelan, Myint, Melissa, Seidl, Katherine

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Cell engineering with microfluidic squeezing preserves functionality of primary immune cells in vivo von DiTommaso, Tia, Cole, Julie M., Cassereau, Luke, Buggé, Joshua A., Hanson, Jacquelyn L. Sikora, Bridgen, Devin T., Stokes, Brittany D., Loughhead, Scott M., Beutel, Bruce A., Gilbert, Jonathan B., Nussbaum, Kathrin, Sorrentino, Antonio, Toggweiler, Janine, Schmidt, Tobias, Gyuelveszi, Gabor, Bernstein, Howard, Sharei, Armon

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Abstract 1524: Peripheral blood mononuclear cells engineered via microfluidic Cell Squeeze® technology to generate APCs that drive mutant-KRas-specific CD8+ T cell responses von Yarar, Defne, Smith, Carolyne, Ramakrishnan, Amritha, Loughhead, Scott, Sharei, Armon, Seidl, Katherine, Bernstein, Howard

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