Ubiquitin diGLY Proteomics as an Approach to Identify and Quantify the Ubiquitin-Modified Proteome von Fulzele, Amit, Bennett, Eric J.

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Enzymatic Assembly of Ubiquitin Chains von Michel, Martin A., Komander, David, Elliott, Paul R.

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Measuring the Overall Rate of Protein Breakdown in Cells and the Contributions of the Ubiquitin-Proteasome and Autophagy-Lysosomal Pathways von Sha, Zhe, Zhao, Jinghui, Goldberg, Alfred L.

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Exploring the Regulation of Proteasome Function by Subunit Phosphorylation von VerPlank, Jordan J. S., Goldberg, Alfred L.

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Characterization of RING-Between-RING E3 Ubiquitin Transfer Mechanisms von Reiter, Katherine H., Klevit, Rachel E.

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Native Gel Approaches in Studying Proteasome Assembly and Chaperones von Roelofs, Jeroen, Suppahia, Anjana, Waite, Kenrick A., Park, Soyeon

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Recombinant Expression, Unnatural Amino Acid Incorporation, and Site-Specific Labeling of 26S Proteasomal Subcomplexes von Bard, Jared A. M., Martin, Andreas

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Ubiquitin-Activated Interaction Traps (UBAITs): Tools for Capturing Protein-Protein Interactions von O’Connor, Hazel F., Swaim, Caleb D., Canadeo, Larissa A., Huibregtse, Jon M.

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Exploring the Rampant Expansion of Ubiquitin Proteomics von Rose, Amalia, Mayor, Thibault

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Methods to Rapidly Prepare Mammalian 26S Proteasomes for Biochemical Analysis von Kuo, Chueh-Ling, Collins, Galen Andrew, Goldberg, Alfred L.

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Measurement of the Multiple Activities of 26S Proteasomes von Kim, Hyoung Tae, Collins, Galen Andrew, Goldberg, Alfred L.

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Generating Intracellular Modulators of E3 Ligases and Deubiquitinases from Phage-Displayed Ubiquitin Variant Libraries von Zhang, Wei, Sidhu, Sachdev S.

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Scalable In Vitro Proteasome Activity Assay von Singh Gautam, Amit Kumar, Martinez-Fonts, Kirby, Matouschek, Andreas

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Dissecting Dynamic and Heterogeneous Proteasome Complexes Using In Vivo Cross-Linking-Assisted Affinity Purification and Mass Spectrometry von Wang, Xiaorong, Huang, Lan

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Single-Turnover RING/U-Box E3-Mediated Lysine Discharge Assays von Buetow, Lori, Gabrielsen, Mads, Huang, Danny T.

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Strategies to Trap Enzyme-Substrate Complexes that Mimic Michaelis Intermediates During E3-Mediated Ubiquitin-Like Protein Ligation von Streich, Frederick C., Lima, Christopher D.

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A Method to Monitor Protein Turnover by Flow Cytometry and to Screen for Factors that Control Degradation by Fluorescence-Activated Cell Sorting von Comyn, Sophie A., Mayor, Thibault

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Using In Vitro Ubiquitylation Assays to Estimate the Affinities of Ubiquitin-Conjugating Enzymes for Their Ubiquitin Ligase Partners von Hill, Spencer, Hill, Connor, Kleiger, Gary

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E. coli-Based Selection and Expression Systems for Discovery, Characterization, and Purification of Ubiquitylated Proteins von Levin-Kravets, Olga, Keren-Kaplan, Tal, Attali, Ilan, Sharon, Itai, Tanner, Neta, Shapira, Dar, Rathi, Ritu, Persaud, Avinash, Shohat, Noa, Shusterman, Anna, Prag, Gali

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Interpreting the Language of Polyubiquitin with Linkage-Specific Antibodies and Mass Spectrometry von Matsumoto, Marissa L., Castellanos, Erick R., Zeng, Yi Jimmy, Kirkpatrick, Donald S.

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