Cell-free gene expression: an expanded repertoire of applications von Silverman, Adam D., Karim, Ashty S., Jewett, Michael C.

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A cell-free framework for rapid biosynthetic pathway prototyping and enzyme discovery von Karim, Ashty S., Jewett, Michael C.

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Use of expression-enhancing terminators in Saccharomyces cerevisiae to increase mRNA half-life and improve gene expression control for metabolic engineering applications von Curran, Kathleen A., Karim, Ashty S., Gupta, Akash, Alper, Hal S.

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Metabolic engineering of muconic acid production in Saccharomyces cerevisiae von Curran, Kathleen A., Leavitt, John M., Karim, Ashty S., Alper, Hal S.

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Carbon-negative production of acetone and isopropanol by gas fermentation at industrial pilot scale von Liew, Fungmin Eric, Nogle, Robert, Abdalla, Tanus, Rasor, Blake J., Canter, Christina, Jensen, Rasmus O., Wang, Lan, Strutz, Jonathan, Chirania, Payal, De Tissera, Sashini, Mueller, Alexander P., Ruan, Zhenhua, Gao, Allan, Tran, Loan, Engle, Nancy L., Bromley, Jason C., Daniell, James, Conrado, Robert, Tschaplinski, Timothy J., Giannone, Richard J., Hettich, Robert L., Karim, Ashty S., Simpson, Séan D., Brown, Steven D., Leang, Ching, Jewett, Michael C., Köpke, Michael

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Cell-free metabolic engineering: Biomanufacturing beyond the cell von Dudley, Quentin M., Karim, Ashty S., Jewett, Michael C.

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Total in vitro biosynthesis of the nonribosomal macrolactone peptide valinomycin von Zhuang, Lei, Huang, Shuhui, Liu, Wan-Qiu, Karim, Ashty S., Jewett, Michael C., Li, Jian

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In vitro prototyping of limonene biosynthesis using cell-free protein synthesis von Dudley, Quentin M., Karim, Ashty S., Nash, Connor J., Jewett, Michael C.

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Cell-free prototyping enables implementation of optimized reverse β-oxidation pathways in heterotrophic and autotrophic bacteria von Vögeli, Bastian, Schulz, Luca, Garg, Shivani, Tarasava, Katia, Clomburg, James M., Lee, Seung Hwan, Gonnot, Aislinn, Moully, Elamar Hakim, Kimmel, Blaise R., Tran, Loan, Zeleznik, Hunter, Brown, Steven D., Simpson, Sean D., Mrksich, Milan, Karim, Ashty S., Gonzalez, Ramon, Köpke, Michael, Jewett, Michael C.

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Toward sustainable, cell-free biomanufacturing von Rasor, Blake J, Vögeli, Bastian, Landwehr, Grant M, Bogart, Jonathan W, Karim, Ashty S, Jewett, Michael C

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Design of synthetic yeast promoters via tuning of nucleosome architecture von Curran, Kathleen A., Crook, Nathan C., Karim, Ashty S., Gupta, Akash, Wagman, Allison M., Alper, Hal S.

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In vitro prototyping and rapid optimization of biosynthetic enzymes for cell design von Karim, Ashty S., Dudley, Quentin M., Juminaga, Alex, Yuan, Yongbo, Crowe, Samantha A., Heggestad, Jacob T., Garg, Shivani, Abdalla, Tanus, Grubbe, William S., Rasor, Blake J., Coar, David N., Torculas, Maria, Krein, Michael, Liew, FungMin (Eric), Quattlebaum, Amy, Jensen, Rasmus O., Stuart, Jeffrey A., Simpson, Sean D., Köpke, Michael, Jewett, Michael C.

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Computationally-guided design and selection of high performing ribosomal active site mutants von Kofman, Camila, Watkins, Andrew M, Kim, Do Soon, Willi, Jessica A, Wooldredge, Alexandra C, Karim, Ashty S, Das, Rhiju, Jewett, Michael C

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Cell-free styrene biosynthesis at high titers von Grubbe, William S., Rasor, Blake J., Krüger, Antje, Jewett, Michael C., Karim, Ashty S.

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Cell-Free Gene Expression: Methods and Protocols von Karim, Ashty S, Jewett, Michael C

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Characterization of plasmid burden and copy number in Saccharomyces cerevisiae for optimization of metabolic engineering applications von Karim, Ashty S., Curran, Kathleen A., Alper, Hal S.

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A rapid cell-free expression and screening platform for antibody discovery von Hunt, Andrew C., Vögeli, Bastian, Hassan, Ahmed O., Guerrero, Laura, Kightlinger, Weston, Yoesep, Danielle J., Krüger, Antje, DeWinter, Madison, Diamond, Michael S., Karim, Ashty S., Jewett, Michael C.

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Spacer2PAM: A computational framework to guide experimental determination of functional CRISPR-Cas system PAM sequences von Rybnicky, Grant A, Fackler, Nicholas A, Karim, Ashty S, Köpke, Michael, Jewett, Michael C

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A Cell-Free Gene Expression Platform for Discovering and Characterizing Stop Codon Suppressing tRNAs von Seki, Kosuke, Galindo, Joey L., Karim, Ashty S., Jewett, Michael C.

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Controlling cell-free metabolism through physiochemical perturbations von Karim, Ashty S., Heggestad, Jacob T., Crowe, Samantha A., Jewett, Michael C.

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