Standardization of cytokine flow cytometry assays von Maecker, Holden T, Rinfret, Aline, D'Souza, Patricia, Darden, Janice, Roig, Eva, Landry, Claire, Hayes, Peter, Birungi, Josephine, Anzala, Omu, Garcia, Miguel, Harari, Alexandre, Frank, Ian, Baydo, Ruth, Baker, Megan, Holbrook, Jennifer, Ottinger, Janet, Lamoreaux, Laurie, Epling, C Lorrie, Sinclair, Elizabeth, Suni, Maria A, Punt, Kara, Calarota, Sandra, El-Bahi, Sophia, Alter, Gailet, Maila, Hazel, Kuta, Ellen, Cox, Josephine, Gray, Clive, Altfeld, Marcus, Nougarede, Nolwenn, Boyer, Jean, Tussey, Lynda, Tobery, Timothy, Bredt, Barry, Roederer, Mario, Koup, Richard, Maino, Vernon C, Weinhold, Kent, Pantaleo, Giuseppe, Gilmour, Jill, Horton, Helen, Sekaly, Rafick P

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(A) Representative manual analysis of a CEF-stimulated sample from Experiment 4 von Maecker, Holden T, Rinfret, Aline, D'Souza, Patricia, Darden, Janice, Roig, Eva, Landry, Claire, Hayes, Peter, Birungi, Josephine, Omu Anzala, Garcia, Miguel, Harari, Alexandre, Frank, Ian, Baydo, Ruth, Baker, Megan, Holbrook, Jennifer, Ottinger, Janet, Lamoreaux, Laurie, C Lorrie Epling, Sinclair, Elizabeth, Suni, Maria A, Punt, Kara, Calarota, Sandra, El-Bahi, Sophia, Gailet Alter, Maila, Hazel, Kuta, Ellen, Cox, Josephine, Gray, Clive, Altfeld, Marcus, Nougarede, Nolwenn, Boyer, Jean, Tussey, Lynda, Tobery, Timothy, Bredt, Barry, Roederer, Mario, Koup, Richard, Maino, Vernon C, Weinhold, Kent, Pantaleo, Giuseppe, Gilmour, Jill, Horton, Helen, Rafick P Sekaly

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Cytokine-positive cells in the absence of stimulus are expressed as a percentage of CD4or CD8T cells von Maecker, Holden T, Rinfret, Aline, D'Souza, Patricia, Darden, Janice, Roig, Eva, Landry, Claire, Hayes, Peter, Birungi, Josephine, Omu Anzala, Garcia, Miguel, Harari, Alexandre, Frank, Ian, Baydo, Ruth, Baker, Megan, Holbrook, Jennifer, Ottinger, Janet, Lamoreaux, Laurie, C Lorrie Epling, Sinclair, Elizabeth, Suni, Maria A, Punt, Kara, Calarota, Sandra, El-Bahi, Sophia, Gailet Alter, Maila, Hazel, Kuta, Ellen, Cox, Josephine, Gray, Clive, Altfeld, Marcus, Nougarede, Nolwenn, Boyer, Jean, Tussey, Lynda, Tobery, Timothy, Bredt, Barry, Roederer, Mario, Koup, Richard, Maino, Vernon C, Weinhold, Kent, Pantaleo, Giuseppe, Gilmour, Jill, Horton, Helen, Rafick P Sekaly

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Cytokine-positive cells in response to CEF peptides or CMV pp65 peptide mix are expressed as a percentage of CD4or CD8T cells von Maecker, Holden T, Rinfret, Aline, D'Souza, Patricia, Darden, Janice, Roig, Eva, Landry, Claire, Hayes, Peter, Birungi, Josephine, Omu Anzala, Garcia, Miguel, Harari, Alexandre, Frank, Ian, Baydo, Ruth, Baker, Megan, Holbrook, Jennifer, Ottinger, Janet, Lamoreaux, Laurie, C Lorrie Epling, Sinclair, Elizabeth, Suni, Maria A, Punt, Kara, Calarota, Sandra, El-Bahi, Sophia, Gailet Alter, Maila, Hazel, Kuta, Ellen, Cox, Josephine, Gray, Clive, Altfeld, Marcus, Nougarede, Nolwenn, Boyer, Jean, Tussey, Lynda, Tobery, Timothy, Bredt, Barry, Roederer, Mario, Koup, Richard, Maino, Vernon C, Weinhold, Kent, Pantaleo, Giuseppe, Gilmour, Jill, Horton, Helen, Rafick P Sekaly

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Comparative results are shown with backgrounds subtracted; no significant differences in backgrounds were seen with liquid versus lyophilised reagents (data not shown) von Maecker, Holden T, Rinfret, Aline, D'Souza, Patricia, Darden, Janice, Roig, Eva, Landry, Claire, Hayes, Peter, Birungi, Josephine, Omu Anzala, Garcia, Miguel, Harari, Alexandre, Frank, Ian, Baydo, Ruth, Baker, Megan, Holbrook, Jennifer, Ottinger, Janet, Lamoreaux, Laurie, C Lorrie Epling, Sinclair, Elizabeth, Suni, Maria A, Punt, Kara, Calarota, Sandra, El-Bahi, Sophia, Gailet Alter, Maila, Hazel, Kuta, Ellen, Cox, Josephine, Gray, Clive, Altfeld, Marcus, Nougarede, Nolwenn, Boyer, Jean, Tussey, Lynda, Tobery, Timothy, Bredt, Barry, Roederer, Mario, Koup, Richard, Maino, Vernon C, Weinhold, Kent, Pantaleo, Giuseppe, Gilmour, Jill, Horton, Helen, Rafick P Sekaly

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A linear relationship between S von Maecker, Holden T, Rinfret, Aline, D'Souza, Patricia, Darden, Janice, Roig, Eva, Landry, Claire, Hayes, Peter, Birungi, Josephine, Omu Anzala, Garcia, Miguel, Harari, Alexandre, Frank, Ian, Baydo, Ruth, Baker, Megan, Holbrook, Jennifer, Ottinger, Janet, Lamoreaux, Laurie, C Lorrie Epling, Sinclair, Elizabeth, Suni, Maria A, Punt, Kara, Calarota, Sandra, El-Bahi, Sophia, Gailet Alter, Maila, Hazel, Kuta, Ellen, Cox, Josephine, Gray, Clive, Altfeld, Marcus, Nougarede, Nolwenn, Boyer, Jean, Tussey, Lynda, Tobery, Timothy, Bredt, Barry, Roederer, Mario, Koup, Richard, Maino, Vernon C, Weinhold, Kent, Pantaleo, Giuseppe, Gilmour, Jill, Horton, Helen, Rafick P Sekaly

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Error bars represent SEM of the 6 sites participating von Maecker, Holden T, Rinfret, Aline, D'Souza, Patricia, Darden, Janice, Roig, Eva, Landry, Claire, Hayes, Peter, Birungi, Josephine, Omu Anzala, Garcia, Miguel, Harari, Alexandre, Frank, Ian, Baydo, Ruth, Baker, Megan, Holbrook, Jennifer, Ottinger, Janet, Lamoreaux, Laurie, C Lorrie Epling, Sinclair, Elizabeth, Suni, Maria A, Punt, Kara, Calarota, Sandra, El-Bahi, Sophia, Gailet Alter, Maila, Hazel, Kuta, Ellen, Cox, Josephine, Gray, Clive, Altfeld, Marcus, Nougarede, Nolwenn, Boyer, Jean, Tussey, Lynda, Tobery, Timothy, Bredt, Barry, Roederer, Mario, Koup, Richard, Maino, Vernon C, Weinhold, Kent, Pantaleo, Giuseppe, Gilmour, Jill, Horton, Helen, Rafick P Sekaly

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