Implementation of a Multiplex and Quantitative Proteomics Platform for Assessing Protein Lysates Using DNA-Barcoded Antibodies von Lee, Jinho, Geiss, Gary K., Demirkan, Gokhan, Vellano, Christopher P., Filanoski, Brian, Lu, Yiling, Ju, Zhenlin, Yu, Shuangxing, Guo, Huifang, Bogatzki, Lisa Y., Carter, Warren, Meredith, Rhonda K., Krishnamurthy, Savitri, Ding, Zhiyong, Beechem, Joseph M., Mills, Gordon B.

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Quantitative Comparison of Long-wavelength Alexa Fluor Dyes to Cy Dyes: Fluorescence of the Dyes and Their Bioconjugates von Berlier, Judith E, Rothe, Anca, Buller, Gayle, Bradford, Jolene, Gray, Diane R, Filanoski, Brian J, Telford, William G, Yue, Stephen, Liu, Jixiang, Cheung, Ching-Ying, Chang, Wesley, Hirsch, James D, Beechem, Joseph M, Haugland, Rosaria P, Haugland, Richard P

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Easily reversible desthiobiotin binding to streptavidin, avidin, and other biotin-binding proteins: uses for protein labeling, detection, and isolation von Hirsch, James D, Eslamizar, Leila, Filanoski, Brian J, Malekzadeh, Nabi, Haugland, Rosaria P, Beechem, Joseph M, Haugland, Richard P

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120 Human immuno-oncology proteome atlas enables a holistic proteomic approach for spatial biology discovery in FFPE tissues von Rosenbloom, Alyssa, Bonnett, Shilah, Conner, Mark, Piazza, Erin, Filanoski, Brian, Meredith, Rhonda, Yi, Hye Son, Hamanishi, Lori, Ignacio, Eduardo, Nelson, Nadine, Prater, Michael, Kang, Christine, Isgur, Lesley, Hoang, Margaret, Geiss, Gary, Beechem, Joseph

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Nanowire-transistor based ultra-sensitive DNA methylation detection von Maki, Wusi C., Mishra, Nirankar N., Cameron, Eric G., Filanoski, Brian, Rastogi, Shiva K., Maki, Gary K.

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207 The spatially-resolved proteomic atlas of the tumor immune architecture of HER2-positive breast cancer and response to neoadjuvant anti-HER2 therapy-based chemotherapy von Chumsri, Saranya, Kachergus, Jennifer, Shi, Ji, Liu, Yi, Ma, Yaohua, Rosenbloom, Alyssa, Bonnett, Shilah, Conner, Mark, Piazza, Erin, Filanoski, Brian, Meredith, Rhonda, Kang, Christine, Isgur, Lesley, Hoang, Margaret, Geiss, Gary, Beechem, Joseph, Thompson, EA

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An Ancient Retrovirus-like Element Contains Hot Spots for SINE Insertion von Cantrell, Michael A, Filanoski, Brian J, Ingermann, Angela R, Olsson, Katherine, DiLuglio, Nicole, Lister, Zach, Wichman, Holly A

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Abstract 4749: Simultaneous multi-omic measurement of nucleic-acids and proteins at 800-plex using single-molecule optical barcodes: Application to cancer immunotherapy von Beechem, Joseph M., Geiss, Gary, Filanoski, Brian, Birditt, Brian

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A complete pipeline for high‐plex spatial proteomic profiling and analysis of neural cell phenotypes on the CosMx™ Spatial Molecular Imager and AtoMx™ Spatial Informatics Platform... von Rosenbloom, Alyssa, Bonnett, Shilah, Conner, Mark, Phan‐Everson, Tien, Lewis, Zachary, Ong, Giang, Liang, Yan, Brown, Emily, Pan, Liuliu, Wardhani, Aster, Korukonda, Mithra, Brown, Carl, Dunaway, Dwayne, Zhao, Edward, McGuire, Dan, Woo, Sangsoon, Filanoski, Brian, Meredith, Rhonda, Chantranuvatana, Kan, Birditt, Brian, Yi, Hye Son, Piazza, Erin, Reeves, Jason, Kang, Christine, Geiss, Gary, Beechem, Joseph M.

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Abstract 5350: A comprehensive and integrated approach to genomic and proteomic analysis of FFPE NSCLC tumor specimens von Hinerfield, Douglas, Mellen, Jennifer, Geiss, Gary, Webster, Philippa, Merritt, Chris, Barker, Kristi, Demirkan, Gokhan, Filanoski, Brian, Polakiewicz, Roberto, Crosby, Katherine

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An Assay for Simultaneous Profiling of Gene Expression, Phospho- and Total Protein Abundance, and Somatic DNA Mutations for Hematology-Oncology Research von Ross, Paul Martin, Demirkan, Gokhan, Birditt, Brian, Bogatzi, Lisa, Carter, Warren, Filanoski, Brian, Garber, Jessica, Houglum, Aaron, Ignacio, Eduardo, Krouse, Mike, Kuhar, Jamie R., Mashadi-Hossein, Afshin, Dennis, Lucas, Meredith, Gavin, Geiss, Gary, McKay-Fleisch, Jill, Pansalawatta, Mekala, Phan, Joseph, Piazza, Erin, Wheeler, Bryson, Zhou, Denise

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Abstract 5563: 3D Biology™ view of cancer: Simultaneous detection of somatic DNA mutations and expression profiling of genes and signaling proteins from melanoma tumor FFPE samples... von Lee, Jinho, Vellano, Christopher P., Meredith, Gavin, Mckay-Fleisch, Jill, Ross, P. Martin, Tetzlaff, Michael, Reuben, Alexandre, Hudgens, Courtney, Wargo, Jennifer, Garber, Jessica, White, Andrew, Pan, Joseph, Krouse, Mike, Pansalawatta, Mekala, Dennis, Lucas, Kharkia, Anisha, Piazza, Erin, Mashadi-Hossein, Afshin, Boykin, Rich, Elliott, Nathan, Filanoski, Brian, Demirkan, Gokhan, Warren, Sarah, Geiss, Gary, Kim, Dae, Beechem, Joseph, Mills, Gordon B.

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Abstract 4617: A complete pipeline for high-plex spatial proteomic profiling and analysis on the cosmxtm spatial molecular imager and atomtm spatial informatics platform von Phan-Everson, Tien, Lewis, Zachary, Ong, Giang, Liang, Yan, Brown, Emily, Pan, Liuliu, Wardhani, Aster, Korukonda, Mithra, Brown, Carl, Dunaway, Dwayne, Zhao, Edward, McGuire, Dan, Woo, Sangsoon, Rosenbloom, Alyssa, Filanoski, Brian, Meredith, Rhonda, Chantranuvatana, Kan, Birditt, Brian, Yi, Hye Son, Piazza, Erin, Reeves, Jason, Lyssand, John, Devgan, Vik, Rhodes, Michael, Geiss, Gary, Beechem, Joseph

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Abstract 5641: Single cell spatial molecular imaging of 76-plex proteins in clinical cancer samples in response to personalized treatment von Lewis, Zachary R., Birditt, Brian, Brown, Emily, Chantranuvatana, Kan, Filanoski, Brian, Corless, Chris, Phan-Everson, Tiên, Geiss, Gary, Hether, Tyler, Hobbs, Evie, Johnson, Brett, Kelley, Taylor, Lopez, Charles, Meredith, Rhonda, Olson, Anastasiya, Ong, Giang, Korukonda, Mithra, Piazza, Erin, Reeves, Jason, Rosenbloom, Alyssa, Siex, Kiara, Yi, Hye Son, Zhao, Edward, Beechem, Joseph M., Mills, Gordon

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147 Comprehensive in situ immune phenotyping with the CosMx™ high plex protein assay von Phan-Everson, Tien, Chen, Jianji, Lewis, Zachary, Geiss, Gary, Liang, Yan, Brown, Emily, Pan, Liuliu, Phelan, Stefan, Glaser, Charlie, Korukonda, Mithra, Brown, Carl, Dunaway, Dwayne, Phan, Joseph, Rosenbloom, Alyssa, Filanoski, Brian, Meredith, Rhonda, Kan Chantranuvatana, Birditt, Brian, Ong, Giang, Hye Son Yi, Piazza, Erin, Reeves, Jason, Basu, Subham, Kang, Christine, Devgan, Vik, Zhao, Edward, Rhodes, Michael, Beechem, Joseph

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Abstract 3878: Subcellular characterization of over 100 proteins in FFPE tumor biopsies with CosMx Spatial Molecular Imager von Lewis, Zachary R., Phan-Everson, Tien, Geiss, Gary, Korukonda, Mithra, Bhatt, Ruchir, Brown, Carl, Dunaway, Dwayne, Phan, Joseph, Rosenbloom, Alyssa, Filanoski, Brian, Meredith, Rhonda, Chantranuvatana, Kan, Liang, Yan, Brown, Emily, Birditt, Brian, Ong, Giang, Yi, Hye Son, Piazza, Erin, Devgan, Vikram, Ortogero, Nicole, Danaher, Patrick, Warren, Sarah, Rhodes, Michael, Beechem, Joseph

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Ultra-sensitive detection of bacterial toxin with silicon nanowire transistor von Mishra, Nirankar N, Maki, Wusi C, Cameron, Eric, Nelson, Ron, Winterrowd, Paul, Rastogi, Shiva K, Filanoski, Brian, Maki, Gary K

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Abstract A013: Biomarker development for cancer immuno-oncology/immunotherapy: Simultaneous digital counting of nucleic acids and proteins at 800-plex von Beechem, Joseph, Manrao, Liz, Ross, Marty, Demirkan, Gokhan, Filanoski, Brian, Birditt, Brian, Ngouenet, Celine, Bailey, Christina, Dennis, Lucas, Pekker, Irena, Meredith, Gavin, Kim, Dae, Giess, Gary

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METHODS OF PURIFYING ANTIBODY-NUCLEIC ACID CONJUGATES von GEISS, Gary, FILANOSKI, Brian

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Universal bio-molecular signal transduction-based nano-electronic bio-detection system von Maki, Wusi C., Mishra, Nirankar N., Rastogi, Shiva K., Cameron, Eric, Filanoski, Brian, Winterrowd, Paul, Maki, Gary K.

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